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BIOFUEL PRODUCTION TECHNOLOGY USING MIXED-LIQUID, MIXED-SPECIES CULTURING meetings

Foreign code F160008882
File No. (S2015-0747-N0)
Posted date Oct 25, 2016
Country WIPO
International application number 2016JP057691
International publication number WO 2016140374
Date of international filing Mar 4, 2016
Date of international publication Sep 9, 2016
Priority data
  • P2015-043817 (Mar 5, 2015) JP
Title BIOFUEL PRODUCTION TECHNOLOGY USING MIXED-LIQUID, MIXED-SPECIES CULTURING meetings
Abstract The purpose of the present invention is to provide a novel technology for producing biofuel, and, specifically, the present invention relates to a biofuel manufacturing method that includes coculturing of mixed microorganisms including photosynthetic microorganisms and non-photosynthetic microorganisms by using a nitrogen-source deprived medium.
Outline of related art and contending technology BACKGROUND ART
Fossil fuel energy (natural gas, petroleum, coal or the like) and in view of the current depends on Japan, future, and of the fuel by itself can be produced by a technique is important. Therefore, the water and carbon dioxide is fixed by photosynthesis, can be an organic substance such as glucose to produce biofuel using photosynthetic organisms to produce very inside Japan attempt has attracted attention. Including plasfocjanin (prokaryotic photosynthetic microorganisms) photosynthetic microorganisms, which belong to the microalgae in the broader sense, photosynthesis ability as compared to that of the plant is one hundred times to several tens are said to be. In addition, in the case of using the microalgae biofuel production, such as rhizomes or other cultivated plant as compared to after the unnecessary waste does not arise from the fact that, as used in the next generation biofuel production has been regarded as promising organism. However, the biofuel is, as a raw material of the biodiesel or fatty acid triglyceride (triacylglycerol, TAG), roughly divided into such as a hydrocarbon. 2 The former one oxygen molecule (O) whose chemical structure has a, hydrocarbon and hydrogen (H) carbon (C) only and, as a fuel to the engine and the environmentally friendly been favored in petroleum industry. And hydrocarbon fuel having a carbon number of the double bond connecting them by the number and position, defined its properties as a fuel. A chain contain double bonds among the saturated hydrocarbons are, alkane (Cn H2n+2) referred to, to the short-chain alkane is 1-20 carbon atoms, various introduction we serves as a fuel. In particular carbon number alkane is 12-17 or 11-17, which are liquids at standard (at normal temperature and pressure) and, as a fuel light oil positioned corresponding to the jet fuel. Up to now of microalgae, some species of cyanobacteria in natural algae, having a carbon number of 15 (pentadecane, C15 H32) or 17 (heptadecanoic, c17 H36) alkane producing strain (non-patent document 1) are known. Group is the present inventors, it was modified by genetic manipulation of natural algae cyanobacteria, per cell dry weight up to about 50-60% mutants are successfully acquired (Patent Document 1). Not limited to this natural algae and in genetic recombination of the microalgae for biofuel production using algae has been attempted. Those in the prior art, isolated was purified algal cell in a culture medium were sterilized in a sterile or aseptically by culturing while approaching, the bio fuel into and out of the algae have been produced. In addition, as can be seen in bioethanol production of articles, produced from the plant after collection of the glucose, to obtain the target 2 by yeast fermentation production stage compared with the technology, is advantageous in using photosynthetic microorganisms, from glucose as described above for the purpose of the organic material (bio-fuel and the like) stage 1 (one within the bioreactor were cultured at the same time) can be produced according to this invention. On the other hand, useful substance production by co-culture as an example of the conventional method, , bacteria and yeast famentasu co-culture system for methyl halides (Patent Document 2), the culture medium wherein the green astika karius bo trio lactococcus, eccentric bird kas strain was added and the co-culture system to promote the proliferation of green algae (Patent Document 3), hydrolyzed from a biomass feedstock in a system for ethanol fuel Clostridial, further comprising phyto fell men chest of drawers or other fine cells by co-culture method (patent document 4) and the like.
Scope of claims (In Japanese)[請求項1]
光合成微生物と非光合成微生物とを含む混合微生物を、窒素源欠乏培地において共培養することを含む、バイオ燃料の製造方法。
[請求項2]
光合成微生物がハロミクロネマ(Halomicronema)属に属する微生物である、請求項1記載の方法。
[請求項3]
ハロミクロネマ属に属する微生物が、受託番号NITE BP-01982で特定されるハロミクロネマ・エスピー(Halomicronema sp.)SZ2菌株又は糖生産能を有するその変異株である、請求項2記載の方法。
[請求項4]
非光合成微生物がシノリゾビウム(Sinorhizobium)属に属する微生物である、請求項1~3のいずれか1項記載の方法。
[請求項5]
シノリゾビウム属に属する微生物が、受託番号NITE BP-01981で特定されるシノリゾビウム・エスピー(Sinorhizobium sp.)ST1菌株又は短鎖アルカン生産能を有するその変異株である、請求項4記載の方法。
[請求項6]
培地が無機物培地と有機物培地とをさらに含有する、請求項1~5のいずれか1項記載の方法。
[請求項7]
培地が炭素源をさらに含有する、請求項1~6のいずれか1項記載の方法。
[請求項8]
炭素源が酢酸ナトリウムである、請求項7記載の方法。
[請求項9]
培地が糖源をさらに含有する、請求項1~8のいずれか1項記載の方法。
[請求項10]
糖源がグルコースである、請求項9記載の方法。
[請求項11]
共培養において所定の暗黒期間を設ける、請求項1~10のいずれか1項記載の方法。
[請求項12]
共培養後、共培養物を乾燥ストレス下で更に培養することを含む、請求項1~11のいずれか1項記載の方法。
[請求項13]
バイオ燃料が短鎖アルカンである、請求項1~12のいずれか1項記載の方法。
[請求項14]
短鎖アルカンがヘプタデカンである、請求項13記載の方法。
[請求項15]
受託番号NITE BP-01982で特定されるハロミクロネマ・エスピーSZ2菌株又は糖生産能を有するその変異株。
[請求項16]
受託番号NITE BP-01981で特定されるシノリゾビウム・エスピーST1菌株又は短鎖アルカン生産能を有するその変異株。
[請求項17]
請求項15記載のハロミクロネマ・エスピーSZ2菌株又は糖生産能を有するその変異株と請求項16記載のシノリゾビウム・エスピーST1菌株又は短鎖アルカン生産能を有するその変異株とを含む混合微生物。
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • IBARAKI UNIVERSITY
  • Inventor
  • ASAYAMA Munehiko
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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