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TRANSPLANT FISH PRODUCTION METHOD, TRANSPLANT FISH, HYBRID FISH SPECIES PRODUCTION METHOD, AND HYBRID FISH SPECIES

Foreign code F160008910
File No. (S2015-0859-N0)
Posted date Nov 29, 2016
Country WIPO
International application number 2016JP059551
International publication number WO 2016153019
Date of international filing Mar 25, 2016
Date of international publication Sep 29, 2016
Priority data
  • P2015-065152 (Mar 26, 2015) JP
Title TRANSPLANT FISH PRODUCTION METHOD, TRANSPLANT FISH, HYBRID FISH SPECIES PRODUCTION METHOD, AND HYBRID FISH SPECIES
Abstract This transplant fish production method includes selecting a donor fish species, selecting first and second fish species which are a combination that could be sterile, producing a hybrid fish species of the selected first fish species and second fish species, and transplanting reproductive cells of the donor fish species into the produced hybrid fish species.
Outline of related art and contending technology BACKGROUND ART
In recent years, natural resources continues to gradually decreasing trend of the amount of fishing is a fishing, saltwater fishing is the total volume ratio in accordance with this increase in the percentage of the amount of production has been increased. On the other hand, in the culture of marine fish, natural product to a size of the still often the case that the developing seedling. For this reason, together with increase of the importance of resource management, fish farming completely introduced more strongly demanded.
Aquaculture is complete, a target fish from fertilized eggs of the seedling produced artificially, the obtained artificial seedling, distributed as a product, further in addition to this, the resulting artificial seedling and some individuals of the parent to fish cultivation, producing gametes, gametes obtained that it is utilized for culturing the, natural resources are completed without depending on the style refers to aquaculture. In order to realize the aquaculture completely first, for the purpose of the parent fish from the fish, both the quality and quantity to obtain the sufficient seedling must be gametes. Seedling to obtain the sufficient gametes, the maintenance of the parent fish of sufficient size and housing facility, suitable according to the present and growing is indispensable and the induction of egg laying, a lot of space and effort. For this reason, many farmer can easily be implemented to the extent that the use of seedling production to establish said fish species is small.
As a technique which can solve this problem, the surrogate host fish technique has been attracting attention as (surrogate broodstock technology). The present technique, so as to obtain the gametes of the donor and target fish species, for the production of gametes is attempted and the recipient fish species, undifferentiated germ cells of the donor and recipient, the recipient fish method is used as a surrogate host. Undifferentiated germ cells as donors, primordial germ cells, spermatogonia or oogonia illustrated in cells. The present technique, in other words, the gonads of recipient donor germ cells in the differentiation or proliferation by, to produce gametes of the donor, then, the next generation of donor to produce a population of individuals technique. In the case of fish, and male gametes is motility, sperm termed, female gametes is no motility, referred to as egg. Surrogate host fish in that technique, in the case of obtaining the sperm, the male recipient transplanted donor germ cells, on the other hand, in the case of obtaining the egg is, female germ cell into a recipient of the donor.
Surrogate host fish techniques include, for example, the 2003-235558 and the publication JP Fisheries for Global Welfare and Environment, 5th World Fisheries Congress 2008 (2008) p209-219, landlocked (masu salmon, Oncorhynchus masaou) spermatogonia of the donor, recipient of the same family of the same group as the male and female fin-implanted egg of the landlocked (rainbow trout, Oncorhynchus mykiss) by obtaining sperm, and the next generation of surrogate host of fin-fish or landlocked a method for the production of the individual is.
Science Vol.317 (2007) p1517 is, 3 times to produce a rainbow trout and rainbow trout of sperm and egg derived from acid to produce fish of the surrogate host may be sterile, the sterility of the germ cells of the surrogate host or landlocked fish in an efficient manner by implanting a sperm and the egg to obtain the landlocked, a method for the production of the next generation individual landlocked described. In addition, JP Patent Application 2006-101845, 3 to produce a haploid or landlocked and, to this, a method of transferring primordial germ cells derived from a fin is disclosed. According to these methods, and/or sperm derived from the recipient egg is hardly formed as it is not, a number of recipients of gametes of the donor gametes from the pick up a complexity that can be reduced, the gametes of the donor efficiently can be obtained and described.
Is Fisheries Science Vol.77 (2011) p69-77, spermatogonia of the debris when implanted in a drumfish (nive broaker, Nibea mitsujurrii), drumfish be captured of the gonads, did not result in the production of gametes is described. Biology of Reproduction Vol.82 (2010) p896-904 is, spermatogonia of a drumfish when transplanted into a chub mackerel, drumfish is taken into the germ cells to the gonad chub mackerel, proliferating is also confirmed, and it was not for the production of gametes is described.
Scope of claims (In Japanese)[請求項1]
ドナー魚種を選択すること、
不稔となり得る組み合わせである第一及び第二の魚種を選択すること、
選択された第一の魚種と第二の魚種とのハイブリッド魚種を作出すること、及び、
作出されたハイブリッド魚種に、ドナー魚種の生殖細胞を移植すること、
を含む、移植魚の作出方法。
[請求項2]
第一の魚種と第二の魚種は、ドナー魚種に対して共に同科異種である請求項1記載の移植魚の作出方法。
[請求項3]
ハイブリッド魚種が、生殖細胞欠損の表現型を示す請求項1又は請求項2記載の移植魚の作出方法。
[請求項4]
第一の魚種が、ドナー魚種と同科同族異属である請求項1~請求項3のいずれか1項記載の移植魚の作出方法。
[請求項5]
ドナー魚種がサバ科である請求項1~請求項4のいずれか1項記載の移植魚の作出方法。
[請求項6]
第一の魚種及び第二の魚種が、共にマグロ属である、請求項1~請求項5のいずれか1項記載の移植魚の作出方法。
[請求項7]
第一の魚種及び第二の魚種が、共にスマ属である、請求項1~請求項5のいずれか1項記載の移植魚の作出方法。
[請求項8]
ドナー魚種が、海産魚、養殖魚、又は海産魚且つ養殖魚である、請求項1~請求項7のいずれか1項記載の移植魚の作出方法。
[請求項9]
請求項1~請求項8のいずれか1項記載の方法を用いて作出され得る、移植魚。
[請求項10]
請求項1~請求項8のいずれか1項記載の方法を用いて移植魚を得ること、及び、
得られた移植魚から、ドナー魚種由来の配偶子を得ること、
を含む、魚類の配偶子の生産方法。
[請求項11]
ドナー魚種を選択すること、
不稔となり得る組み合わせである第一及び第二の魚種を選択すること、及び、
選択された第一の魚種と、第二の魚種とのハイブリッド魚種を作出すること、
を含み、ハイブリッド魚種が代理親魚技法用に用いられ得る、ハイブリッド魚種の作出方法。
[請求項12]
第一の魚種と第二の魚種は、ドナー魚種に対して共に同科異種である請求項11記載のハイブリッド魚種の作出方法。
[請求項13]
第一の魚種が、ドナー魚種と同科同族異属である請求項11又は請求項12記載のハイブリッド魚種の作出方法。
[請求項14]
ドナー魚種が、サバ科である、請求項11~請求項13のいずれか1項記載のハイブリッド魚種の作出方法。
[請求項15]
第一の魚種と第二の魚種が、共にマグロ属である、請求項11~請求項14のいずれか1項記載のハイブリッド魚種の作出方法。
[請求項16]
第一の魚種と第二の魚種が、共にスマ属である、請求項11~請求項14のいずれか1項記載のハイブリッド魚種の作出方法。
[請求項17]
作出されるハイブリッド魚種が、生殖細胞欠損の表現型を示す請求項11~請求項16のいずれか1項記載のハイブリッド魚種の作出方法。
[請求項18]
請求項11~請求項16のいずれか1項記載の方法により得られ得るハイブリッド魚種。
[請求項19]
生殖細胞欠損の表現型を示す請求項18記載のハイブリッド魚種。
[請求項20]
請求項10記載の方法で生産され得る、配偶子。
[請求項21]
請求項9記載の移植魚から得られ得る、種苗。
[請求項22]
請求項1~請求項8のいずれか1項記載の方法により移植魚を得ること、
得られた移植魚から種苗を得ること、
を含む種苗の生産方法。
[請求項23]
請求項1~請求項8のいずれか1項記載の方法により移植魚を得ること、
得られた移植魚から種苗を得ること、
得られた種苗を成魚まで育成すること、
を含む成魚の育成方法。
[請求項24]
請求項23記載の育成方法により得られ得る、成魚。
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • NATIONAL UNIVERSITY CORPORATION TOKYO UNIVERSITY OF MARINE SCIENCE AND TECHNOLOGY
  • Inventor
  • YOSHIZAKI Goro
  • TAKEUCHI Yutaka
  • YAZAWA Ryosuke
  • KAWAMURA Wataru
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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