TOP > 外国特許検索 > TECHNIQUE FOR AGGREGATING MACROMOLECULES TOGETHER WITH CELLS

TECHNIQUE FOR AGGREGATING MACROMOLECULES TOGETHER WITH CELLS UPDATE

外国特許コード F170008919
整理番号 (S2015-1519-N0)
掲載日 2017年1月19日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP064167
国際公開番号 WO 2016182022
国際出願日 平成28年5月12日(2016.5.12)
国際公開日 平成28年11月17日(2016.11.17)
優先権データ
  • 特願2015-099064 (2015.5.14) JP
発明の名称 (英語) TECHNIQUE FOR AGGREGATING MACROMOLECULES TOGETHER WITH CELLS UPDATE
発明の概要(英語) Provided is a technique for efficiently aggregating macromolecules such as ECM together with cells. A method for creating a cell or a cell aggregate loaded with macromolecules, the method comprising adding, to a medium containing a swellable material, a solution containing macromolecules and at least one cell to aggregate the macromolecules together with the cell. A method for controlling the function and/or the property of a cell or a cell aggregate, the method comprising culturing the cell or the cell aggregate loaded with the macromolecules created in the aforementioned method. A method for culturing a cell or a cell aggregate, the method comprising: adding, to a medium containing a swellable material, a solution containing macromolecules and at least one cell to aggregate the macromolecules together with the cell and to produce a macromolecule capsule encapsulating the cell or the cell aggregate; and culturing the cell or the cell aggregate in the capsule. For preparing a three-dimensional cell tissue, methods such as 96-well U-bottom plating and hanging drop technique are conventionally used. In the methods of the present invention, 2000 cells are mixed with 1 μl of the medium containing ECM, and the ECM and cells can be simultaneously aggregated. If the efficiency here is defined as 1, a calculation can be made that, when 96-well U-bottom plating or hanging drop technique is used, ECM can only be mixed in the three-dimensional cell tissue at an efficiency of about 1/12000 or about 1/2400, respectively. Thus, with the present invention, the cost for ECM can be reduced to about one several-thousandth to one hundred-thousandth, and it becomes possible to effectively use artificial ECM that cannot be synthesized in large quantity or rare ECM that can be extracted only at a very small amount because of reasons such as the subject being a small living organism.
従来技術、競合技術の概要(英語) BACKGROUND ART
Cells were used for cell assays or in regenerative medicine, cells were three-dimensional organization of cells in order to enhance the ability to differentiate is considered to be an effective means. Is a general three-dimensional organization, cell clumps in order to make the non-adhesive plate or the hanging drop method using (Lin et al., Biotechnology Journal, 2008,3,1172-1184: non-patent document 1). However, as is required for the regenerative medicine and in drug screening, with high added value than for production of three-dimensional tissue, which further devised method for constructing a three-dimensional tissue is demanded.
We present in the body of the organs, cells and extracellular matrix and laminated Mn. Previously and in the extracellular matrix (ECM) on the cell surface which is a kind of gelatin and fibronectin coating, laminating technique (Nishiguchi et al., Advanced Materials, 2011, 23, 3506-3510: non-patent document 2, Matsusaki et al., Angewandte Chemie 2007, 46, 4689-4692: non-patent document 3) is reported.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITY
  • 発明者(英語)
  • KOJIMA, Nobuhiko
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
※ ライセンスをご希望の方、特許の内容に興味を持たれた方は、問合せボタンを押してください。

PAGE TOP

close
close
close
close
close
close