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ANALYTICAL DEVICE UPDATE コモンズ

外国特許コード F170008930
掲載日 2017年1月19日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP058447
国際公開番号 WO 2016152702
国際出願日 平成28年3月17日(2016.3.17)
国際公開日 平成28年9月29日(2016.9.29)
優先権データ
  • 特願2015-060845 (2015.3.24) JP
発明の名称 (英語) ANALYTICAL DEVICE UPDATE コモンズ
発明の概要(英語) A device is provided with which a multiplex detection for a specimen can be conducted by means of one optical system. By disposing, in one or more channels, microstructures each comprising a photocured hydrophilic resin and a specific binding reagent supported therein, the device capable of multiplex detection can be manufactured while avoiding reagent mixing during the device manufacture.
従来技術、競合技術の概要(英語) BACKGROUND ART
The affinity of the antibody to the antigen used as an immunoassay utilizing the specific binding analysis method, clinical examinations and in the prior art, have been widely used for drug discovery. An amount of the analyte reagent used can be avoided and even a small amount, the time required for the assay can be shortened, so-called 96 - well plate in place of the assay using such a plate, a smaller capacity of the reaction system using the micro device has been developed in the assay.
Further, than the use of the plate as a carrier, by using the flow path, which reduces the time required for the assay. The present inventors, the hydrophilic photo-curable resin in the beads with the immobilized antibody are homogeneously dispersed as a pillar-shaped structure is held in the immunological analysis placed in the flow path of the microfluidic device has been disclosed (Patent Document 1, Non-Patent Document 1).
Fig. 8A is, on the order of 1 μm diameter polystyrene micro-beads immobilized on the primary antibody, a photocurable resin in the flow path of a small amount of the pillar-shaped structure are provided in the microfluidic device. The micro fluidic devices are, the primary antibody was immobilized on the micro beads suspended in a hydrophilic photo-curable resin solution, in suspension in the flow path is patterned by the exposure was being filled with a hardened device analysis of a single item.
This device is, for use in the following manner. First, the serum in the flow path, an analyte such as urine is satisfied, the incubation, the sample contained in the antigen bound to the primary antibody on the beads. After washing with the cleaning liquid sample, a fluorescence-labeled antibody was injected into the incubation, the primary antibody is bound to the binding of the antigen is detected. Then, a fluorescence-labeled antibody is not bound by the cleaning solution after washing, by the fluorescence detector to detect the fluorescence of the labeled antibody.
In addition, is shown in Fig. 8B, once the three different antigens can be detected in the assay are shown in the three items of an analysis device. This device is, 3 types of antibodies recognizing a different antigen immobilized on microscopic beads were mixed and suspended in a light-curing resin, and in the same manner as the exposure processing in the flow path is fixed by curing.
In this method, different antibodies on a solid phase of the three micro-beads, in one structure of the pillar-shaped fixed. Which of the three different binding a fluorescent dye and detected using a secondary antibody. At this time, the same in the structure of the three antigens, from the fluorescent label can bind, by a fluorescent dye can be used to detect different wavelengths, excited needs to be used. In addition, each of the signal from fluorescent label is mixed with the excitation spectrum is not observed, a good separation of the fluorescence spectrum is required to use a fluorescent label. For example, detection of antibody used as a fluorescent label, FITC, Alexa FLUOR 555 (trademark), a fluorescent dye such as DyLight 650 (trademark) needs to be used.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITY
  • NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY
  • 発明者(英語)
  • KASAMA Toshihiro
  • BABA Yoshinobu
  • TOKESHI Manabu
  • NISHIWAKI Nanako
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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