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NOVEL METHOD FOR DETECTING PROTEIN OR PATHOGEN UPDATE 新技術説明会

外国特許コード F170008936
整理番号 (S2015-0897-N0)
掲載日 2017年1月19日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP062081
国際公開番号 WO 2016175049
国際出願日 平成28年4月15日(2016.4.15)
国際公開日 平成28年11月3日(2016.11.3)
優先権データ
  • 特願2015-093132 (2015.4.30) JP
発明の名称 (英語) NOVEL METHOD FOR DETECTING PROTEIN OR PATHOGEN UPDATE 新技術説明会
発明の概要(英語) Provided is a novel method for detecting a protein or a pathogen. The present invention pertains to a conductive diamond electrode wherein an element recognizing a protein or a pathogen is immobilized on the surface, and a method for detecting a protein or a pathogen using the conductive diamond electrode. More specifically, the present invention pertains to a diamond electrode wherein an element recognizing a virus or a protein thereof is immobilized on the surface, and a method for detecting a virus using the diamond electrode.
従来技術、競合技術の概要(英語) BACKGROUND ART
Conventionally, the target pathogen, pathogenic bacteria, viruses and their respective proteins can be detected with high sensitivity have been desired. E.g. influenza virus (IFV) pandemics is the possibility, the detection of influenza virus quickly and accurately is demanded. IFV currently used as a method of detecting, using antibodies as the recognition device IFV immuno, using an array of sugar chains sugar chains detected, RT-PCR method using a gene, a hemagglutination assay using red blood cells and the like. However they are time and cost consuming, require expert knowledge or techniques.
Grabowska et al. using IFV hybridization detection methods are reported (Non-Patent Document 1). This method is via a thiol bond gold Au surface of the electrode, 2 is fixed to the two different oligonucleotide probes and the sensor is used, the hemagglutinin (HA) and neuraminidase (NA) both of the oligonucleotide target can be detected at the same time. Further Kamikawa et al., is covered with the electrical activity such as polyaniline, modified HA-H5N1 monoclonal antibody on the surface of the magnetic nanoparticles is used to report the detection IFV (Non-Patent Document 2). In this method, the virus H5N1 in interaction with the nanoparticles (MPC) in a strong magnetic force is recovered from the serum, the degree of electrochemical measurement is quantified by the virus.
Sakurai et al., fluorescence chromatography is used for rapid and sensitive of a seasonal flu typing was reported (Non-Patent Document 3). IFV clinical have been used in a method of detecting the detection sensitivity in the immunochromatography and 1000pfu, it is difficult to early detection of infection. Sakurai et al. in the publication, and a sensitizing agent of the virus antibody supplemented with labeled detection antibody with both the capture of the sandwich-type virus, densitometry analysis apparatus and fluorescence immunochromatography measuring device is further used is detected by using the conventional 100 times the sensitivity of the reported attained. However, a complementary oligonucleotide hybridization or antibody immobilized on the substrate, requires a huge manufacturing cost, the problem of storage stability.
Hassen et al., Electrochemical impedance spectroscopy was used to quantify the influenza A virus has been reported (Non-Patent Document 4). This document the antibody on the gold electrode surface to remove the sugar chain - - avidin fixing the thiol structure using devices, the influenza A virus is detected.
On the other hand the boron doped diamond electrode, glassy carbon or platinum electrodes and the like other conventional electrode materials of excellent and, in recent years, and have garnered attention. Can be a high thermal conductivity is much higher and hardness in addition to the well known properties of diamond, the boron doped diamond electrodes, large potential window, low background current, and the suction resistance is high, such as chemically inert to the attractive properties. In addition, the boron doped diamond electrode may be physically, chemically stable and excellent in durability.
Diamond electrode is provided with the sensor, an accurate determination of the catechol or catechol derivative is possible to perform the diamond electrode and the diamond sensor electrodes have been reported (Patent Document 1). This document also discloses that the modified diamond electrode 4 - pentenoic acid and oxalic acid is used which is the electrochemical detection.
Virus as well as various pathogenic bacteria, also for the pathogen, the antibody and the expensive device is not used, a high-sensitivity detection method has been desired.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • KEIO UNIVERSITY
  • 発明者(英語)
  • SATO Toshinori
  • MATSUBARA Teruhiko
  • EINAGA Yasuaki
  • YAMAMOTO Takashi
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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