TOP > 外国特許検索 > METHOD FOR PRODUCING TRANSFORMED CELL

METHOD FOR PRODUCING TRANSFORMED CELL

外国特許コード F170008938
整理番号 (S2015-0632-N0)
掲載日 2017年1月20日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP061420
国際公開番号 WO 2016163466
国際出願日 平成28年4月7日(2016.4.7)
国際公開日 平成28年10月13日(2016.10.13)
優先権データ
  • 特願2015-078489 (2015.4.7) JP
発明の名称 (英語) METHOD FOR PRODUCING TRANSFORMED CELL
発明の概要(英語) The purpose of the present invention is to provide an easy and high-throughput method whereby a DNA can be transferred between different hosts. To achieve this purpose, provided is a method for producing a transformed cell of a yeast or Escherichia coli , said method being characterized in that the yeast or Escherichia coli is transformed using a vector-containing liquid which contains (A) a lytic solution containing a Bacillus subtilis lysate and a vector released therefrom and (B) additive(s) selected from the group consisting of a nuclease inhibitor, a protein-denaturing agent, a nuclease and a combination of two or more of the same.
従来技術、競合技術の概要(英語) BACKGROUND ART
Recombinant DNA in an experiment, between different host DNA as a technique of moving, transformed, via phage transduction, and the bonding transfer on the method. For example, in the case of using a transformation method, different host replication using a shuttle plasmid vector capable, in a host that was copied in the first DNA 1 once collected, then purified by use of a general-purpose. However, in this method, to recover DNA in a host such as 1 of the first, essential step for purification, a large number of operation and the period of time. Further, the size of DNA is large, it is very difficult to recover and purify DNA is known to be expressed and, to be recovered in the target DNA may not be completely.
The first DNA 2 moving to the host, the host of the first DNA 1 does not need to be recovered and purified from as a method, the first host 1 is a way to protoplasts. However, in this method, the operation of protoplasts for control is necessary in slightly, but anyone is conducted in a simple method, they are less general (non-patent document 1). Further, simply mixing 2 only one host DNA bond capable of moving the transfer method using a (non-patent document 2) is also known, due to the very limited host range is difficult to handle, a large number of genes involved in the bonding to precisely control is required, generally-used method does not.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • TOKYO INSTITUTE OF TECHNOLOGY
  • KEIO UNIVERSITY
  • 発明者(英語)
  • KANEKO SHINYA
  • ITAYA MITSUHIRO
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
詳しくお知りになりたい方は下記「問合せ先」まで直接お問合わせください

PAGE TOP

close
close
close
close
close
close