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METHOD FOR PRODUCING COENZYME AND TRANSFORMANT SET FOR COENZYME PRODUCTION 新技術説明会

外国特許コード F170008942
整理番号 (AF30P003)
掲載日 2017年1月20日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP054872
国際公開番号 WO 2016136620
国際出願日 平成28年2月19日(2016.2.19)
国際公開日 平成28年9月1日(2016.9.1)
優先権データ
  • 特願2015-033843 (2015.2.24) JP
発明の名称 (英語) METHOD FOR PRODUCING COENZYME AND TRANSFORMANT SET FOR COENZYME PRODUCTION 新技術説明会
発明の概要(英語) The invention provides a method for synthesizing NAD+ or NADH in an enzyme reaction system that requires NAD+ or NADH to supplement the NAD+ lost due to thermal decomposition in the enzyme reaction system, and a transformant set for coenzyme production to be used in this method. Specifically, the invention is a method for producing a coenzyme characterized by synthesizing NAD+ or NADH in an enzyme reaction system that requires NAD+ or NADH by adding one or more heat-resistant enzymes necessary for the reaction to synthesize NAD+ from nicotinamide in the enzyme reaction, and a transformant set for coenzyme production characterized by comprising one or more transformants having a heat-resistant microorganism as the host, obtained by introducing a gene that encodes one or more heat-resistant enzymes necessary for the reaction to synthesize NAD+ or NADH from nicotinamide.
従来技術、競合技術の概要(英語) BACKGROUND ART
Genetically modified with the development of technology in recent years, the synthesis reaction is used as a reaction system of the microorganism, the microorganism is a useful organic compound using a method of producing metabolic pathway, in terms of industrial mass production is being used. Further, more efficiently in order to synthesize an organic compound, the metabolism of live microorganisms rather than modifying the path, is located in the module and a plurality of metabolic enzymes, by randomly combining these specialized substance production it is possible to construct artificial synthetic route (artificial metabolic system) has also been attempted. And a method using an artificial metabolic system by, for example, glucose or glycerol and lactic acid, malic acid, 1 - selectively and butanol can be produced in high yields has been reported (for example, see non-patent document 1.).
Used in the form of a module is a metabolic enzyme (enzyme module), the microorganism has been introduced a gene encoding a metabolic enzymes produced by the transformant, can be provided at low cost and easily. Among them, a module enzyme, physical and chemical stability is excellent and the, appropriate for industrial uses, it is preferred to use a heat-resistant enzyme. A method of producing a heat-resistant enzyme by the microorganism include, for example, the target acid-fast bacilli of the non-heat-resistant heat-resistant enzyme gene has been introduced for culturing the transformant and the transformant produced after the heat-resistant enzyme, the cells were cultured by performing heat treatment, the death of the transformants in their body having a heat-resistant immobilized enzyme has been disclosed a method for obtaining a (for example, see Patent Document 1.). In this method, the protein from the host the acid-fast bacilli and all the deactivated, the heat resistance of the object in a state in which retains the activity of the enzyme only to the death of the transformants in their body since the immobilized, the reaction system as it is sterile death even when, due to the enzyme derived from a host have the advantage that the sub-reaction does not occur.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • HONDA KOHSUKE
  • ATOMI HARUYUKI
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
参考情報 (研究プロジェクト等) CREST Creation of Basic Technology for Improved Bioenergy Production through Functional Analysis and Regulation of Algae and Other Aquatic Microorganisms AREA
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