MULTIFOCAL SPECTROSCOPIC MEASUREMENT DEVICE, AND OPTICAL SYSTEM FOR MULTIFOCAL SPECTROSCOPIC MEASUREMENT DEVICE
|発明の名称 （英語）||MULTIFOCAL SPECTROSCOPIC MEASUREMENT DEVICE, AND OPTICAL SYSTEM FOR MULTIFOCAL SPECTROSCOPIC MEASUREMENT DEVICE|
|発明の概要（英語）||Provided is a multifocal spectroscopic measurement device with which it is possible to simultaneously measure a plurality of samples at high sensitivity, with no limitation as to the magnification factor. This multifocal spectroscopic measurement device 10 disperses light by introducing into a spectrograph signal light that is emitted from a plurality of prescribed observation areas in a sample S arranged in a sample arrangement part (sample holder 13), the device comprising a plurality of objective lenses (objective focusing parts) 111, a single one of which is provided at each of locations corresponding to optical systems of the plurality of observation areas, and spectrograph input units 151, a single one of which is provided in corresponding fashion to each of the plurality of objective lenses 111, the spectograph input units 151 inputting signal light that has passed through the corresponding objective lenses 111 to a spectrograph 17. Because each of the objective lenses 111 individually observes only a single observation area, the magnification factor can be increased and the numerical aperture NA enlarged. In so doing, the quantity of light collected by the objective lenses 111 in proportion to the quantity of light of all signal light emitted by the sample S in the observation areas is increased, and the measurement accuracy is higher.|
In recent years, as a center in the pharmaceutical field, high throughput screening (High Throughput Screening: HTS) called, multi-sample analysis of a sample a short time a method for simultaneous measurement of attention. Multi-sample simultaneous measurement method, dispersing the light signal emitted from each sample for each sample by a spectrum, the composition of each sample spectrum in order to analyze the molecular structure and the like, the spectral measurement that is widely used. Signal lights, the light is irradiated on the sample by irradiating the Raman light or fluorescence emitted from each sample, the sample or the sample without irradiation light emitted from the chemiluminescence and the like.
Non-Patent Document 1 and Patent Document 1 is, the coincidence method is a multi-sample have not been described, in the application to multi-sample simultaneous measurement method is a possibility that the spectral measuring device is a multi-focus has been described. The apparatus' Raman spectroscopic microscope ' kind of what is termed a, the later divided into a plurality of irradiation light by the microlens array, each of the irradiation light 1 can be irradiated to the different positions of the three samples obtained by the position of each of the dispersed Raman light, the intensity of light of the Raman shift by mapping, corresponding to the Raman image is acquired as the distribution of the sample components. Here, a plurality of illumination light 1 on different positions of the three samples instead of irradiation, the irradiated laser beams 1 different from each other by irradiating the sample one by one, is applied to the simultaneous measurement of a plurality of samples can be considered.
Using Fig. 15, Patent Document 1 and Non-Patent Document 1 a multi-focus spectral measurement apparatus will be described in detail. In the spectral measurement device 90 of the multi-focus, generated by the laser light source 91 the laser light, the micro lens 921 and horizontally arranged in a matrix 8 one by one by the micro lens array 92 is divided into a plurality of light beams. Each of these light beams, reflected by the edge filter 93, each of the light beams 1 corresponding to the four holes are provided in the pinhole array 94, as well as the relay lens 95 common to each of the light beams passing through the objective lens 96 and, applied to the sample S. From the sample S, the illumination light is divided to correspond to the plurality of light beams, the emitted signal light of the light beams. The signal light, and reflected light by the irradiation light having the same wavelength, the illumination light is superimposed on the Raman light of a different wavelength. The signal light is, the objective lens 96, a relay lens 95, passes through a hole 94 and a pinhole array, reaches the edge filter 93. In the edge filter 93, by the difference in wavelength, the reflected light is reflected without passing through, to pass only Raman light. Passes through the edge filter 93 the Raman light beam, a single lens made from the set of lenses 2 1 after passing through the optical system 97, the number of micro lenses 921 in phase with the optical fiber bundle 92 of the micro lens array is incident on the fiber bundle 98. A plurality of optical fibers in the fiber bundle 98 has an input terminal and arranged in a matrix, each of the input terminals 1 to 1 the Raman light is incident on the light beam. 1 The output end of the fiber bundle 98 are aligned, the light emitted from each output end 99 is dispersed by a spectroscope.
To Patent Document 2, a single objective lens 1 from the irradiation light is applied to the sample S, the signal light emitted from the sample S (Raman light) in the fiber bundle is received by a plurality of optical fibers, each optical fiber from the output end of the light emitted by the spectrometer and the multi-point simultaneous spectroscopic measurement apparatus is described. In this apparatus, the illuminating light is not divided, the fiber bundle and the signal light to reach the point that it is not divided, Patent Document 1 and non-patent document 1 differs from that of the apparatus. In addition, also in Patent Document 2, the coincidence method is a multi-sample has not been described.
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|参考情報 （研究プロジェクト等）||ERATO SODEOKA Live Cell Chemistry AREA|
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