Alga, method for producing thereof and method for producing biomass by using the alga
| 外国特許コード | F170008986 |
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| 整理番号 | AF21-03TW |
| 掲載日 | 2017年3月14日 |
| 出願国 | 台湾 |
| 出願番号 | 104117313 |
| 公報番号 | 201600602 |
| 公報番号 | I671400 |
| 出願日 | 平成27年5月29日(2015.5.29) |
| 公報発行日 | 平成28年1月1日(2016.1.1) |
| 公報発行日 | 令和元年9月11日(2019.9.11) |
| 優先権データ |
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| 発明の名称 （英語） | Alga, method for producing thereof and method for producing biomass by using the alga |
| 発明の概要（英語） | The productivity of biomass within the cells of algae can be improved by suppressing the expression of ATG8 in algae by (i) overexpression of MEX1 and/or (ii) silencing using miRNA, thereby providing algae having improved productivity of photosynthetic products, a method for producing said algae, a method for producing a biomass using said algae, and starch obtained by said algae. |
| 特許請求の範囲（英語） |
[claim1] 1. A process for producing biomass that uses a algae that exhibits inhibition through MEX 1 overexpression and ATG 8 over a benchmark strain. [claim2] 2. The invention relates to a method for producing the raw material of a request item 1, which comprises a step of irradiating light irradiating the algae. [claim3] 3. A method for producing the biomass of the request item 1 or 2, which uses a alga with an increased concentration of gluten in the chloroplast compared to the reference strain. [claim4] 4. The invention relates to a method for producing a biomass of claim 3, wherein the aforementioned light irradiation step is carried out in substantially non-nitrogen starvation conditions. [claim5] 5. A process for producing a biomass of claim 4, which does not contain a cell crushing step that disrupts cells of the algae. [claim6] 6. The invention relates to a production method of the raw material of the request item 1, which is a sugar. [claim7] 7. A single-cell alga is a single-cell alga that is inhibited by MEX 1 overexpression and ATG 8 as compared to reference strains. [claim8] 8. A single cell alga, such as a request item 7, is introduced into an exogenous polynucleotide encoding MEX 1. [claim9] 9. Single-cell alga of request item 8, wherein the aforementioned extrinsic polynucleotide is selected from one or more of the groups comprising a) to c): a) a polynucleotide encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 1 or 3; b) a polynucleotide encoding the polypeptide: 1 or a plurality of amines comprising the amino acid sequence shown in SEQ ID NO: 1 or 3. A polypeptide of the amino acid sequence which is deleted, substituted or added, and retains the function of MEX 1; c) a polynucleotide which hybridizes under stringent conditions with the polynucleotide below and encodes a polypeptide that retains the function of MEX 1: polynucleotide comprising a base sequence complementary to the polynucleotide of any of the polynucleotides of the aforementioned a) or b) polynucleotide. [claim10] 10. As in the case of single cell algae of any one of items 7 to 9, the concentration of gluten peptide in chloroplast increased as compared to the reference strain. [claim11] 11. A method for the production of a modified alga comprising the step of inhibiting the expression of ATG 8 which inhibits expression of ATG 8 by overexpression of MEX 1. [claim12] 12. A process for the production of a modified alga, as claimed in claim item 11, comprising increasing the concentration of gluten peptide concentration in chloroplast to increase the concentration of gluten peptide concentration. |
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| 国際特許分類(IPC) |
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| 参考情報 （研究プロジェクト等） | CREST Creation of Innovative Technologies to Control Carbon Dioxide Emissions AREA |
日本語項目の表示
| 発明の名称 | 藻類及びその製造方法、並びに該藻類を用いたバイオマスの製造方法 |
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