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DNA FOR INTRACELLULAR PENETRATION, AND METHOD FOR INTRODUCING TARGET MOLECULE INTO CELL USING SAID DNA 新技術説明会

外国特許コード F170009018
整理番号 (S2015-2057-N0)
掲載日 2017年3月29日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP077449
国際公開番号 WO 2017047750
国際出願日 平成28年9月16日(2016.9.16)
国際公開日 平成29年3月23日(2017.3.23)
優先権データ
  • 特願2015-185295 (2015.9.18) JP
発明の名称 (英語) DNA FOR INTRACELLULAR PENETRATION, AND METHOD FOR INTRODUCING TARGET MOLECULE INTO CELL USING SAID DNA 新技術説明会
発明の概要(英語) The present invention provides: DNA for intracellular penetration; a composition that includes said DNA and a target molecule to be introduced into a cell; a method for introducing the target molecule into the cell using said composition; and an agent and kit for introducing the target molecule into the cell, said agent and kit including said DNA. Provided is DNA for intracellular penetration, said DNA including an intracellular penetration region comprising the following base sequence: 5'-N1-N2-GG-N5-N6-N7-GGTGGT-N14-GGGG-N19-N20-G-N22-N23-TCG-N27-N28-N29-G-N31-AT-N34-N35-GTG-N39-N40-TCG-3' [Herein, N1 is deleted or is T, N2 is G or is deleted, N5 is C or T, N6 is G or C, N7 is G or T, N14 is G or C, N19 is A or G, N20 is G or A, N22 is deleted or is T, N23 is T or A, N27 is G, T, or A, N28 is T, A, or C, N29 is deleted or is T, N31 is G or A, N34 is G or is deleted, N35 is C or A, N39 is G or T, and N40 is G or A] (SEQ ID NO: 1).
従来技術、競合技術の概要(英語) BACKGROUND ART
A nucleic acid or a peptide or protein molecules such as a technique introduced into the cell, or the molecules in the pathogenesis of elucidation of the function of the treatment of a disease or a variety of purposes including in elucidating important. However, in general, a molecule such as a protein or nucleic acid is rendered more hydrophilic, typically, is very small for the cell membrane. Therefore, these molecules can be introduced into a cell have been developed many techniques.
For example, as a technique for introducing nucleic acids into cells, electrically open hole in the cell membrane is incorporated into the nucleic acids into cells include electroporation, direct injection of nucleic acids by means of a glass capillary microinjection method, lipofection method liposomes taken up nucleic acid, such as a retrovirus virus using the viral vectors and the like. However, electroporation and microinjection method, physically leaving a hole in the cell in order to introduce a nucleic acid, significantly damage the cells, in addition, requires a dedicated apparatus. The electroporation method, in accordance with cells and is necessary to consider the conditions for introduction, time-consuming. Is a lipofection method, a problem such as toxicity to the cells of the reagent and, further, and costly. Viral vectors is, it takes much time for the preparation of the vectors, in addition, the introduction of a nucleic acid or a protein other than for the purpose of such a problem that there is a possibility. That is, currently available techniques include, introduction efficiency, cell toxicity, cost, effort, such as in terms of safety are many problems remain.
The nucleic acid drug has been developed rapidly in recent years also, generally difficult to be introduced to in vivo, in a need to develop efficient drug delivery system.
A cell-membrane permeable peptide (CPP) into cells are commonly referred to as a peptide having a property using transition, protein and nucleic acid molecules such as of a method of introducing into cells which are also known. As the representative cell-membrane permeable peptide, and virus-derived Tat HIV-1, and from Drosophila and penetratin (Patent Document 1 and 2).
Similarly, transitions into to the cells having the properties reported RNA (non-patent document 1 and patent document 3). This RNA is, random sequence RNA from the library, the shift to the selected RNA in the cell cycle and amplified by repeating a plurality of times, select only RNA in a cell migration method (referred to as Cell SELEX method) identified by. However, for RNA is generally susceptible to degradation, the intracellular migration using RNA introduced into the cell and other molecules technique into practical use, many challenges including the stability and the like.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • OSAKA CITY UNIVERSITY
  • 発明者(英語)
  • TACHIBANA Akira
  • TANABE Toshizumi
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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