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Vector utilizing borna disease virus and use thereof

Foreign code F170009065
Posted date May 26, 2017
Country United States of America
Application number 201013258698
Gazette No. 20120151614
Gazette No. 9365865
Date of filing Mar 17, 2010
Gazette Date Jun 14, 2012
Gazette Date Jun 14, 2016
International application number JP2010054600
International publication number WO2010113647
Date of international filing Mar 17, 2010
Date of international publication Oct 7, 2010
Priority data
  • P2009-087608 (Mar 31, 2009) JP
  • 2010WO-JP54600 (Mar 17, 2010) WO
Title Vector utilizing borna disease virus and use thereof
Abstract (US9365865)
Disclosed is a viral vector comprising (a) a cDNA of a recombinant viral RNA having at least the N gene, the X gene, the P gene, and the L gene of a Borna disease virus genome in the same order as that in the Borna disease virus genome, and having a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene, (b) a DNA encoding a ribozyme, and (c) a promoter sequence, each being disposed in a position in which (b) is placed upstream and downstream of (a), and (a) and (b) are placed downstream of (c).
Scope of claims [claim1]
1. A viral vector comprising: (a) a cDNA of a recombinant viral RNA having at least the N gene, the X gene, the P gene, and the L gene of a Borna disease virus genome in the same order as that in the Borna disease virus genome,
having a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene,
containing restriction enzyme sites both at the 3' end and at the 5' end of the foreign gene,
containing the sequence shown in SEQ ID NO: 11 (1) both in the region between the restriction enzyme site at the 3' end of the foreign gene and the open reading frame of the P gene and in the region between the restriction enzyme site at the 5' end of the foreign gene and the open reading frame of the L gene, or (2) both in the region between the restriction enzyme site at the 3' end of the foreign gene and the open reading frame of the P gene and the region between the restriction enzyme site at the 5' end of the foreign gene and the open reading frame of the M gene when the M gene of the Borna disease viral genome is contained in the same order as that in the Borna disease viral genome, and
containing at least a base sequence cc inserted in the region between the restriction enzyme site at the 3' end of the foreign gene and the sequence shown in SEQ ID NO: 11, and at least a base sequence cca in the region between the restriction enzyme site at the 5' end of the foreign gene and the sequence shown in SEQ ID NO: 11,
(b) a DNA encoding a ribozyme, and
(c) a promoter sequence,
each being disposed in a position in which (b) is placed upstream and downstream of (a), and (a) and (b) are placed downstream of (c),
wherein (a) the cDNA of a recombinant viral RNA has a sequence in which the G gene and the M gene of the Borna disease virus genome are destructed and has a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene, and
wherein an intron of the L gene of the Borna disease virus genome is deleted from (a) the cDNA of a recombinant viral RNA.
[claim2]
2. The viral vector according to claim 1, wherein the restriction enzyme site at the 3' end of the foreign gene is a Bst BI site, and the restriction enzyme site at the 5' end of the foreign gene is a Pac I site.
[claim3]
3. The viral vector according to claim 1, wherein (c) the promoter sequence is an RNA polymerase II promoter sequence.
[claim4]
4. A kit comprising the viral vector according to claim 1.
[claim5]
5. The viral vector according to claim 1, wherein the foreign gene expresses a functional RNA.
  • Inventor, and Inventor/Applicant
  • TOMONAGA KEIZO
  • DAITO TAKUJI
  • HONDA TOMOYUKI
  • KYOTO UNIVERSITY
IPC(International Patent Classification)
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