Vector utilizing borna disease virus and use thereof
| 外国特許コード | F170009066 |
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| 掲載日 | 2017年5月26日 |
| 出願国 | 欧州特許庁（ＥＰＯ） |
| 出願番号 | 10758423 |
| 公報番号 | 2415866 |
| 公報番号 | 2415866 |
| 出願日 | 平成22年3月17日(2010.3.17) |
| 公報発行日 | 平成24年2月8日(2012.2.8) |
| 公報発行日 | 平成28年8月10日(2016.8.10) |
| 国際出願番号 | JP2010054600 |
| 国際公開番号 | WO2010113647 |
| 国際出願日 | 平成22年3月17日(2010.3.17) |
| 国際公開日 | 平成22年10月7日(2010.10.7) |
| 優先権データ |
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| 発明の名称 （英語） | Vector utilizing borna disease virus and use thereof |
| 発明の概要（英語） |
(EP2415866) Disclosed is a viral vector comprising (a) a cDNA of a recombinant viral RNA having at least the N gene, the X gene, the P gene, and the L gene of a Borna disease virus genome in the same order as that in the Borna disease virus genome, and having a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene, (b) a DNA encoding a ribozyme, and (c) a promoter sequence, each being disposed in a position in which (b) is placed upstream and downstream of (a), and (a) and (b) are placed downstream of (c). |
| 特許請求の範囲（英語） |
[claim1] 1. A viral vector comprising (a) a cDNA of a recombinant viral RNA having at least the N gene, the X gene, the P gene, and the L gene of a Borna disease virus genome in the same order as that in the Borna disease virus genome, having a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene, containing restriction enzyme sites both at the 3' end and at the 5' end of the foreign gene, containing the sequences shown in SEQ ID NO: 11 both in the region between the restriction enzyme site at the 3' end of the foreign gene and the open reading frame of the P gene; and in the region between the restriction enzyme site at the 5' end of the foreign gene and the open reading frame of the L gene, or the region between the restriction enzyme site at the 5' end of the foreign gene and the open reading frame of the M gene when the M gene of the Borna disease viral genome is contained in the same order as that in the Borna disease viral genome, and containing at least a base sequence cc inserted in the region between the restriction enzyme site at the 3' end of the foreign gene and the sequence shown in SEQ ID NO: 11, and at least a base sequence cca in the region between the restriction enzyme site at the 5' end of the foreign gene and the sequence shown in SEQ ID NO: 11 (b) a DNA encoding a ribozyme, and (c) a promoter sequence, each being disposed in a position in which (b) is placed upstream and downstream of (a), and (a) and (b) are placed downstream of (c). [claim2] 2. The viral vector according to claim 1, wherein (a) the cDNA of a recombinant viral RNA has a sequence in which the G gene of the Borna disease virus genome is destructed and has a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene. [claim3] 3. The viral vector according to claim 1 or 2, wherein (a) the cDNA of a recombinant viral RNA has a sequence in which the G gene and the M gene of the Borna disease virus genome are destructed and has a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene. [claim4] 4. The viral vector according to any of claims 1 to 3, wherein the restriction enzyme site at the 3' end of the foreign gene is a Bst BI site, and the restriction enzyme site at the 5' end of the foreign gene is a Pac I site. [claim5] 5. The viral vector according to any of claims 1 to 4, wherein (c) the promoter sequence is an RNA polymerase II promoter sequence. [claim6] 6. The viral vector according to claim 2 or 3, wherein an intron of the L gene of the Borna disease virus genome is deleted from (a) the cDNA of a recombinant viral RNA. [claim7] 7. A recombinant virus comprising an RNA encoded by the viral vector according to any of claims 1 to 6. [claim8] 8. A process for preparing a recombinant virus, the process comprising the steps of introducing, into an in vitro cell, a plasmid or a plasmid group expressing the N gene, the P gene, and the L gene of a Borna disease virus as a helper plasmid, together with the viral vector according to any of claims 1 to 6, and culturing the cell having the introduced viral vector and helper plasmid to produce a recombinant virus. [claim9] 9. The process for preparing a recombinant virus according to claim 8, wherein a plasmid expressing the coat gene of a virus is further introduced as a helper plasmid into the in vitro cell. [claim10] 10. The process for preparing a recombinant virus according to claim 8 or 9, wherein a plasmid expressing the M gene of a Borna disease virus is further introduced as a helper plasmid into the in vitro cell. [claim11] 11. A process for introducing a foreign gene, the process comprising the step of infecting an in vitro cell with the recombinant virus according to claim 7. [claim12] 12. A foreign gene transfer agent comprising the recombinant virus according to claim 7. [claim13] 13. A foreign gene transfer agent for a cranial nerve system cell, the agent comprising the recombinant virus according to claim 7. [claim14] 14. A kit for introducing a foreign gene, the kit comprising the viral vector according to any of claims 1 to 6. [claim15] 15. The viral vector according to any of claims 1 to 6, wherein the foreign gene is a DNA encoding a functional RNA. |
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| 国際特許分類(IPC) |
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| 指定国 |
(EP2415866) Contracting States: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR |
日本語項目の表示
| 発明の名称 | ボルナ病ウイルスを利用するベクター及びその利用 |
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『 Vector utilizing borna disease virus and use thereof 』に関するお問合せ
- 京都大学「医学領域」産学連携推進機構 技術移転チーム
- URL: http://www.kumbl.med.kyoto-u.ac.jp/index.php
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