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Vector utilizing borna disease virus and use thereof

Foreign code F170009066
Posted date May 26, 2017
Country EPO
Application number 10758423
Gazette No. 2415866
Gazette No. 2415866
Date of filing Mar 17, 2010
Gazette Date Feb 8, 2012
Gazette Date Aug 10, 2016
International application number JP2010054600
International publication number WO2010113647
Date of international filing Mar 17, 2010
Date of international publication Oct 7, 2010
Priority data
  • P2009-087608 (Mar 31, 2009) JP
  • 2010WO-JP54600 (Mar 17, 2010) WO
Title Vector utilizing borna disease virus and use thereof
Abstract (EP2415866)
Disclosed is a viral vector comprising (a) a cDNA of a recombinant viral RNA having at least the N gene, the X gene, the P gene, and the L gene of a Borna disease virus genome in the same order as that in the Borna disease virus genome, and having a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene, (b) a DNA encoding a ribozyme, and (c) a promoter sequence, each being disposed in a position in which (b) is placed upstream and downstream of (a), and (a) and (b) are placed downstream of (c).
Scope of claims [claim1]
1. A viral vector comprising (a) a cDNA of a recombinant viral RNA
having at least the N gene, the X gene, the P gene, and the L gene of a Borna disease virus genome in the same order as that in the Borna disease virus genome,
having a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene,
containing restriction enzyme sites both at the 3' end and at the 5' end of the foreign gene,
containing the sequences shown in SEQ ID NO: 11 both in the region between the restriction enzyme site at the 3' end of the foreign gene and the open reading frame of the P gene; and in the region between the restriction enzyme site at the 5' end of the foreign gene and the open reading frame of the L gene, or the region between the restriction enzyme site at the 5' end of the foreign gene and the open reading frame of the M gene when the M gene of the Borna disease viral genome is contained in the same order as that in the Borna disease viral genome, and
containing at least a base sequence cc inserted in the region between the restriction enzyme site at the 3' end of the foreign gene and the sequence shown in SEQ ID NO: 11, and at least a base sequence cca in the region between the restriction enzyme site at the 5' end of the foreign gene and the sequence shown in SEQ ID NO: 11 (b) a DNA encoding a ribozyme, and (c) a promoter sequence, each being disposed in a position in which (b) is placed upstream and downstream of (a), and (a) and (b) are placed downstream of (c).
[claim2]
2. The viral vector according to claim 1, wherein (a) the cDNA of a recombinant viral RNA has a sequence in which the G gene of the Borna disease virus genome is destructed and has a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene.
[claim3]
3. The viral vector according to claim 1 or 2, wherein (a) the cDNA of a recombinant viral RNA has a sequence in which the G gene and the M gene of the Borna disease virus genome are destructed and has a sequence in which a foreign gene is inserted into the untranslated region connected to the downstream of the open reading frame of the P gene.
[claim4]
4. The viral vector according to any of claims 1 to 3, wherein the restriction enzyme site at the 3' end of the foreign gene is a Bst BI site, and the restriction enzyme site at the 5' end of the foreign gene is a Pac I site.
[claim5]
5. The viral vector according to any of claims 1 to 4, wherein (c) the promoter sequence is an RNA polymerase II promoter sequence.
[claim6]
6. The viral vector according to claim 2 or 3, wherein an intron of the L gene of the Borna disease virus genome is deleted from (a) the cDNA of a recombinant viral RNA.
[claim7]
7. A recombinant virus comprising an RNA encoded by the viral vector according to any of claims 1 to 6.
[claim8]
8. A process for preparing a recombinant virus, the process comprising the steps of
introducing, into an in vitro cell, a plasmid or a plasmid group expressing the N gene, the P gene, and the L gene of a Borna disease virus as a helper plasmid, together with the viral vector according to any of claims 1 to 6, and
culturing the cell having the introduced viral vector and helper plasmid to produce a recombinant virus.
[claim9]
9. The process for preparing a recombinant virus according to claim 8, wherein a plasmid expressing the coat gene of a virus is further introduced as a helper plasmid into the in vitro cell.
[claim10]
10. The process for preparing a recombinant virus according to claim 8 or 9, wherein a plasmid expressing the M gene of a Borna disease virus is further introduced as a helper plasmid into the in vitro cell.
[claim11]
11. A process for introducing a foreign gene, the process comprising the step of infecting an in vitro cell with the recombinant virus according to claim 7.
[claim12]
12. A foreign gene transfer agent comprising the recombinant virus according to claim 7.
[claim13]
13. A foreign gene transfer agent for a cranial nerve system cell, the agent comprising the recombinant virus according to claim 7.
[claim14]
14. A kit for introducing a foreign gene, the kit comprising the viral vector according to any of claims 1 to 6.
[claim15]
15. The viral vector according to any of claims 1 to 6, wherein the foreign gene is a DNA encoding a functional RNA.
  • Applicant
  • KYOTO UNIVERSITY
  • Inventor
  • TOMONAGA KEIZO
  • DAITO TAKUJI
  • HONDA TOMOYUKI
IPC(International Patent Classification)
Specified countries (EP2415866)
Contracting States: AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR
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