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CELL PREPARATION METHOD

Foreign code F170009070
File No. (S2015-2063-N0)
Posted date May 29, 2017
Country WIPO
International application number 2016JP081187
International publication number WO 2017069222
Date of international filing Oct 20, 2016
Date of international publication Apr 27, 2017
Priority data
  • P2015-207529 (Oct 21, 2015) JP
Title CELL PREPARATION METHOD
Abstract Provided is a method for preparing a somatic cell characterized by comprising culturing, in the presence of a TGF-β pathway inhibitor, a differentiated somatic cell of a mammal in a medium for inducing the differentiation of a somatic cell other than the differentiated somatic cell described above and thus converting the differentiated somatic cell into the other somatic cell.
Scope of claims [claim1]
1. The somatic cell where the mammal differentiates, the description above in the nutrient medium in order it differentiates to induce the other somatic cell other than the somatic cell which differentiates, culturing under existing of the TGF-.beta. passway inhibiter, the description above the somatic cell which differentiates the method of featuring that [konvuato] it can point in the other somatic cell, manufacturing the somatic cell.
[claim2]
2. The TGF-.beta. passway inhibiter, is D4476, SB431542, LY2157299, SD208 or ALK5 inhibiter II, in claim 1 method of statement.
[claim3]
3. The TGF-.beta. passway inhibiter, is ALK5 inhibiter II, in claim 1 or 2 method of statement.
[claim4]
4. The tissue bud cell it features that [konvuato] it can point in the cell of mesenchyme type, either of the claim 1-3 in 1 sections method of statement.
[claim5]
5. Tissue bud cell or [kerachinosaito] it features that [konvuato] it can point in the bone bud cell, either of the claim 1-3 in 1 sections method of statement.
[claim6]
6. The tissue bud cell or the peripheral blood single nuclear cell it features that [konvuato] it can point in the white fat cell, either of the claim 1-3 in 1 sections method of statement.
[claim7]
7. Tissue bud cell or [kerachinosaito] it features that [konvuato] it can point in the brown fat cell, either of the claim 1-3 in 1 sections method of statement.
[claim8]
8. The nutrient medium in order it differentiates to induce the somatic cell, Peroxisome Proliferator-Activated Receptor-.gamma. (PPAR-.gamma.) includes [agonisuto], in claim 6 or 7 method of statement.
[claim9]
9. The tissue bud cell it features that [konvuato] it can point in the cartilage cell, either of the claim 1-3 in 1 sections method of statement.
[claim10]
10. The tissue bud cell it features that [konvuato] it can point in the muscle bud cell, either of the claim 1-3 method of 1 section statements.
[claim11]
11. The tissue bud cell it features that [konvuato] it can point in the [shiyuwan] cell, either of the claim 1-3 in 1 sections method of statement.
[claim12]
12. [kerachinosaito] it features that [konvuato] it can point in the urine road surface skin cell, either of the claim 1-3 in 1 sections method of statement.
[claim13]
13. The tissue bud cell it features that [konvuato] it can point in the mesenchyme type trunk cell, either of the claim 1-3 in 1 sections method of statement.
[claim14]
14. The somatic cell which includes the TGF-.beta. passway inhibiter, differentiates the induction medicine because [konvuato] it can point in the other somatic cell.
[claim15]
15. The somatic cell which differentiates being the kit because [konvuato] it can point in the other somatic cell, the kit which includes the nutrient medium in order it differentiates to induce the TGF-.beta. passway inhibiter and the aforementioned other somatic cell.
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • KYOTO PREFECTURAL PUBLIC UNIVERSITY
  • Inventor
  • YAMAMOTO KENTA
  • KISHIDA TSUNAO
  • SOWA YOSHIHIRO
  • YAMAMOTO TOSHIRO
  • MAZDA OSAM
IPC(International Patent Classification)
Specified countries (WO201769222)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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