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METHOD FOR SITE-SPECIFIC INSERTION OF FOREIGN DNA INTO ANIMAL CELL GENOME AND CELL OBTAINED USING SAME

外国特許コード F170009072
整理番号 (S2015-1830-N0)
掲載日 2017年5月29日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP059174
国際公開番号 WO 2017029833
国際出願日 平成28年3月23日(2016.3.23)
国際公開日 平成29年2月23日(2017.2.23)
優先権データ
  • 特願2015-162612 (2015.8.20) JP
  • 201562265425 (2015.12.10) US
発明の名称 (英語) METHOD FOR SITE-SPECIFIC INSERTION OF FOREIGN DNA INTO ANIMAL CELL GENOME AND CELL OBTAINED USING SAME
発明の概要(英語) Provided is a method for site-specific insertion of a foreign DNA into an animal cell genome, said method comprising: (1) a step for constructing a donor plasmid in which 100-300 bp DNAs having a sequence homologous to an insertion site on a genome are ligated to the upstream and downstream of a 0.1-10 kbp foreign DNA; and (2) a step for inserting the 0.1-10 kbp foreign DNA into a target site in the genome by genome editing through homologous recombination repair with the use of the donor plasmid obtained above.
従来技術、競合技術の概要(英語) BACKGROUND ART
In recent years in the genome of a cell of the animal introduced site-specific modification technique, so-called genome editing technology is remarkably progressed. That is, in recent years, Zn-finger nuclease (ZFN), TALEN, genome editing technology such as CRISPR-Cas9 which becomes available, after cutting DNA homologous recombination repair (homology direct repair: HDR) using, a specific portion of the genome of a cell culture insert foreign DNA becomes available. By using this technique, an endogenous gene can be added to a tag such as GFP (non-patent document 1-7) becomes. On the other hand, in the single-stranded oligo DNA of 50-80b can have the homology region, the method of modifying the site-specific sequences have also been developed (non-patent document 7, 8). S2 Fly in a cell, utilizing a primer added homology PCR DNA fragment was amplified, foreign DNA insertion method is used as it is and has been developed (non-patent document 9). On the other hand, after cutting by genome editing HDR genome without using the path, another path can be used in the repair pathway and tagged micro-homology end-joining (MMEJ) have also been developed a technique (non-patent document 10).
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • INTER-UNIVERSITY RESEARCH INSTITUTE CORPORATION RESEARCH ORGANIZATION OF INFORMATION AND SYSTEMS
  • 発明者(英語)
  • KANEMAKI Masato
  • NATSUME Toyoaki
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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