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METHOD FOR IDENTIFYING BIOACTIVE PROTEIN, AND BIOACTIVE PROTEIN OBTAINED BY SAID METHOD

外国特許コード F170009076
整理番号 (S2015-1988-N64)
掲載日 2017年5月29日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP077198
国際公開番号 WO 2017047672
国際出願日 平成28年9月14日(2016.9.14)
国際公開日 平成29年3月23日(2017.3.23)
優先権データ
  • 特願2015-180831 (2015.9.14) JP
発明の名称 (英語) METHOD FOR IDENTIFYING BIOACTIVE PROTEIN, AND BIOACTIVE PROTEIN OBTAINED BY SAID METHOD
発明の概要(英語) The purpose of the present invention is to provide a method for identifying a bioactive protein translated from an open reading frame (ORF) other than a mORF present in mRNA. This problem is solved by a method for identifying a bioactive protein that is characterized by identifying a bioactive protein-encoding ORF other than the main ORF in the mRNA of a eukaryote, said method including: (1) a step in which an expression vector containing a candidate ORF is introduced into a cell, and the vector-introduced cell is cultured; (2) a step in which a protein that binds to a candidate protein, which is translated from the candidate ORF, is detected by immunoprecipitating the candidate protein from the cultured cell; and (3) a step in which the candidate protein, for which another protein that binds to the candidate protein was detected, is determined to be a bioactive protein.
従来技術、競合技術の概要(英語) BACKGROUND ART
Eukaryotic protein gene transcribed from messenger RNA (hereinafter, referred to as mRNA) current, the open reading frame encoding a functional protein (Open Reading Frame: hereinafter, referred to as ORF) only have one to 1, that are considered . This is, having the structure operon, i.e. polycistronic bacterial gene is compared with the described, the principle of the current one large genetics 1. However, is the nucleotide sequence of a eukaryotic mRNA, the start codon and stop codon ORF region resides in the same frame, 1 there are a plurality of on one mRNA. In many mRNA on, one of the plurality of ORF ORF of the 1, significantly longer than the other ORF. In general, this is the longest ORF, physiologically active, biologically active protein having a cell function is believed to be the encoding.
Non-patent document 1 and 2 is, the main ORF (hereinafter, sometimes referred to as mORF) upstream of the initiation codon (upstream ORF: hereinafter, referred to as uORF) ORF is, involved in the regulation of translation mORF are reported. These translation protein, has a function of the translational control of an mORF is, itself does not have physiological activity. That is, from an animal, other than mORF ORF translated from protein with biological activity has not been found.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • TOKYO INSTITUTE OF TECHNOLOGY
  • LIFEIS INC.
  • 発明者(英語)
  • AIZAWA Yasunori
  • KITANO Shohei
  • KIDA Yuichiro
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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