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CULTURE CONTAINER

Foreign code F170009086
File No. (S2016-0034-N0)
Posted date May 30, 2017
Country WIPO
International application number 2016JP082413
International publication number WO 2017078007
Date of international filing Nov 1, 2016
Date of international publication May 11, 2017
Priority data
  • P2015-218569 (Nov 6, 2015) JP
Title CULTURE CONTAINER
Abstract A culture container 1 comprising a culture container body 11, a partition board 15 that is disposed in the vicinity of the bottom surface 11c of the culture container body 11 facing the bottom surface 11c and forms a culture area A1 as a culture area being positioned between the partition board 15 and the bottom surface 11c, and a sample inlet/outlet 19 that is formed in the culture container body 11, wherein the sample inlet/outlet 19 communicates with the culture area A1 via an opening formed on one end side of the partition board 15. By using the culture container 1 and a culture method using the same, a large amount of fat cells can be cultured at once by a simple operation. Thus, a large amount of fat cells can be efficiently dedifferentiated and non-dedifferentiated cells can be efficiently removed.
Scope of claims [claim1]
1. Culture container itself and,
Opposing to the base in the base vicinity of the aforementioned culture container itself, the division plate which is arranged and forms the culture territory with the aforementioned bases and,
The sample coming out entrance which is provided in portion of the aforementioned culture container itself and, having,
Through the open part which was formed to one end side of the aforementioned division plate the aforementioned culture territory and that the aforementioned sample coming out entrance communicates are featured, the culture container.
[claim2]
2. The aforementioned division plate features that it has the edge board which is extended to the aforementioned base side alongside the edge of the aforementioned open part, in claim 1 the culture container of statement.
[claim3]
3. The aforementioned sample coming out entrance the side of the aforementioned open part side of the aforementioned culture features that it is provided on the ceiling surface container or, in claim 1 or 2 the culture container of statement.
[claim4]
4. Clearance of the aforementioned division plate and the aforementioned base features that it is 3.5mm-5mm, either of the claim 1-3 in 1 sections the culture container of statement.
[claim5]
5. The aforementioned division plate features that gluing layer is provided for the aforementioned base side principal plane, either of the claim 1-4 in 1 sections the culture container of statement.
[claim6]
6. The aforementioned gluing layer, raminin and huiburonekuchin, I type collagen, features that it is chosen from the group which consists of the gelatin, in claim 5 the culture container of statement.
[claim7]
7. The aforementioned division plate other than the aforementioned open part in portion of edge side, features that it possesses the air hole, either of the claim 1-6 in 1 sections the culture container of statement.
[claim8]
8. The aforementioned division plate features that it has the edge board which is extended to the aforementioned base side alongside the edge of the aforementioned air hole, in claim 7 the culture container of statement.
[claim9]
9. The aforementioned open part and the aforementioned air hole, in order to oppose on the abbreviation diagonal of the aforementioned division plate mutually, feature that it is provided, either of the claim 3-8 in 1 sections the culture container of statement.
[claim10]
10. The aforementioned culture container the case where dedifferentiation of the fat cell is done features that it is the container for ceiling culture, either of the claim 1-9 in 1 sections the culture container of statement.
[claim11]
11. Opposing to the base in the base vicinity of the culture container, the division plate which is arranged and forms the culture territory with the aforementioned bases, through the open part of the aforementioned culture territory, in the aforementioned culture territory of the culture container which has the sample coming out entrance which is communicated with the aforementioned culture territory, the maturity fat cell and the process which fills up the culture solution and,
The process which makes the aforementioned maturity fat cell which floated the aforementioned division plate glue and,
The process which barely inclines the aforementioned culture container, portion of the culture solution inside the aforementioned culture territory deviation from the maturity fat cell which does not differentiate, through the aforementioned open part, it removes from the aforementioned sample coming out entrance outside the culture container and,
It features that it has, dedifferentiation method of the fat cell.
[claim12]
12. In the aforementioned gluing process, through gluing layer, it features that it makes the aforementioned maturity fat cell the aforementioned division plate glue, in claim 11 dedifferentiation method of the fat cell of statement.
[claim13]
13. The aforementioned inclined angle features that they are 90 degrees or less, in claim 11 or 12 dedifferentiation method of the fat cell of statement.
[claim14]
14. The aforementioned culture container, either of the claim 1-9 features that it is the culture container of statement in 1 sections, in claim 11 dedifferentiation method of the fat cell of statement.
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • NIHON UNIVERSITY
  • Inventor
  • MATSUMOTO TARO
  • KAZAMA TOMOHIKO
  • HAGIKURA KAZUHIRO
IPC(International Patent Classification)
Specified countries (WO201778007)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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