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MYOSATELLITE CELL-CULTURING MATERIAL AND METHOD FOR CULTURING MYOSATELLITE CELL

Foreign code F170009094
File No. (S2016-0028-N0)
Posted date May 30, 2017
Country WIPO
International application number 2016JP082490
International publication number WO 2017078029
Date of international filing Nov 1, 2016
Date of international publication May 11, 2017
Priority data
  • P2015-216609 (Nov 4, 2015) JP
Title MYOSATELLITE CELL-CULTURING MATERIAL AND METHOD FOR CULTURING MYOSATELLITE CELL
Abstract The purpose of the present invention is to provide: a myosatellite cell-culturing material, by means of which cells are proliferated while remaining undifferentiated; and a culturing method using the same. The present invention pertains to: a myosatellite cell-culturing material including laminin; and a method for culturing myosatellite cells by using the same.
Scope of claims [claim1]
1. The muscle satellite cell culture material charge which includes 1st raminin.
[claim2]
2. It is the culture backing material for muscle satellite cell culture or the nutrient medium for muscle satellite cell culture, muscle satellite cell culture material charge of claim 1 statement.
[claim3]
3. 1st raminin is the culture backing material for muscle satellite cell culture which coating is done, claim muscle satellite cell culture material charge of 1 or 2 statements.
[claim4]
4. Aforementioned 1st raminin, either of the claim 1-3 which features that the E8 fragment of hitoraminin is included in one section muscle satellite cell culture material charge of statement.
[claim5]
5. Aforementioned 1st raminin, from the group which consists of hitoraminin .alpha.2.beta.1.gamma.1, hitoraminin .alpha.3.beta.3.gamma.2, hitoraminin .alpha.4.beta.1.gamma.1 and hitoraminin .alpha.5.beta.1.gamma.1 either of the claim 1-4 which features that at least kind of is selected hitoraminin is included in one section muscle satellite cell culture material charge of statement.
[claim6]
6. Aforementioned 1st raminin, either of the claim 1-5 which features that the E8 fragment of hitoraminin .alpha.2.beta.1.gamma.1 is included in one section muscle satellite cell culture material charge of statement.
[claim7]
7. The contact process which makes with muscle satellite cell and 1st raminin is included, culture method of the muscle satellite cell.
[claim8]
8. 1st raminin,
a) It is included in the nutrient medium;
b) It is included in the culture backing material;
Or
c) Coating it is done in the culture backing material
Thing is featured, culture method of the muscle satellite cell of claim 7 statement.
[claim9]
9. The culture backing material, the dish, the plate and cover slip, the micro carrier, features that it is zerachinhaidorogeru or the collagen sponge, culture method of the muscle satellite cell of claim 8 statement.
[claim10]
10. In the aforementioned contact process, either of the claim 1-6 it features that culture material charge of statement is used for one section, in claim 7 culture method of the muscle satellite cell of statement.
[claim11]
11. Furthermore, the contact process which makes with muscle satellite cell and 2nd raminin is included, either of the claim 7-10 culture method of the muscle satellite cell of one section statement.
[claim12]
12. Aforementioned 2nd raminin, features that at least hitoraminin of 1 where it is selected kinds is included from the group which consists of hitoraminin .alpha.3.beta.3.gamma.2, hitoraminin .alpha.4.beta.1.gamma.1 and hitoraminin .alpha.5.beta.1.gamma.1, in claim 11 culture method of statement.
[claim13]
13. Aforementioned 2nd raminin, features that the E8 fragment of hitoraminin is included, in claim 11 or 12 culture method of statement.
[claim14]
14. Aforementioned 2nd raminin, features that it is included in the culture nutrient medium of the muscle satellite cell, either of the claim 11-13 culture method of one section statement.
[claim15]
15. Before the contact process which makes with aforementioned muscle satellite cell and 2nd raminin seeds the muscle satellite cell on the culture backing material it features that it is done, either of the claim 11-14 culture method of one section statement.
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • TOKYO MEDICAL AND DENTAL UNIVERSITY
  • OSAKA UNIVERSITY
  • Inventor
  • AKAZAWA CHIHIRO
  • SUZUKI NOBUHARU
  • MABUCHI YOH
  • ISHII KANA
  • SEKIYA ICHIRO
  • SEKIGUCHI KIYOTOSHI
IPC(International Patent Classification)
Specified countries (WO201778029)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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