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METHOD FOR MEASURING TYROSINE PHOSPHATASE AND TYROSINE KINASE ACTIVITY

外国特許コード F170009095
整理番号 (S2015-1473-N0)
掲載日 2017年5月30日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP078224
国際公開番号 WO 2017061288
国際出願日 平成28年9月26日(2016.9.26)
国際公開日 平成29年4月13日(2017.4.13)
優先権データ
  • 特願2015-198320 (2015.10.6) JP
発明の名称 (英語) METHOD FOR MEASURING TYROSINE PHOSPHATASE AND TYROSINE KINASE ACTIVITY
発明の概要(英語) Provided is a method for measuring tyrosine phosphatase and tyrosine kinase activity, as a high-sensitivity measuring method, which is suitable for high throughput and which uses a compound represented by general formula (I) (in the formula, A represents a conjugated ring; L represents a linker or the like having a labeling substance at an end; R1 represents a hydrogen atom or the like; and R2 and R3 each represent a hydrogen atom, an alkyl group or the like).
従来技術、競合技術の概要(英語) BACKGROUND ART
Tyrosine kinase to phosphorylate a tyrosine residue in proteins is an enzyme, on the other hand tyrosine phosphatase phosphorylated tyrosine residues and a substrate, the enzyme which catalyzes the dephosphorylation of the insulin. Tyrosine kinase and tyrosine phosphatases play an important role in vivo and, abnormality in the expression of these enzymes are often the cause of the disease. For example, tyrosine kinase is EGFR (epidermal growth factor receptor), such as over-expressed in lung cancer or colon cancer and it has been known that, in addition, tyrosine phosphatase PTP1B be related to diabetes have been reported. For this reason, the activity of tyrosine kinase and tyrosine phosphatase is accurately measured, in the treatment of diseases involving these enzymes are important for establishing means.
The method of measuring the tyrosine kinase activity, are known and many, for example,32 Pmethod using a radioactive isotope (non-patent document 1), a method of using anti-phospho-antibody (non-patent document 2), using capillary electrophoresis method (non-patent document 3) and the like are known. On the other hand, for the measurement of the activity of tyrosine phosphatases, not much is known. As the method commonly used, the inorganic phosphate was quantified by the malachite green, then a method for estimating the activity (non-patent document 4) or p- nitrophenyl phosphate (pNPP) and tyrosine phosphatase tyrosine mimic such as phosphorylation reaction, a method of quantifying the dephosphorylated generating (non-patent document 5) is.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • TOKYO INSTITUTE OF TECHNOLOGY
  • 発明者(英語)
  • SATO, Shinichi
  • NAKAMURA, Hiroyuki
  • NAKANO, Hirofumi
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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