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CELL CULTURE VESSEL AND SAMPLE CELL FOR OBSERVATION USE meetings

Foreign code F170009102
File No. (S2015-1988-N47)
Posted date Jun 14, 2017
Country WIPO
International application number 2016JP082979
International publication number WO 2017094451
Date of international filing Nov 7, 2016
Date of international publication Jun 8, 2017
Priority data
  • P2015-237526 (Dec 4, 2015) JP
Title CELL CULTURE VESSEL AND SAMPLE CELL FOR OBSERVATION USE meetings
Abstract The present invention provides a cell culture vessel or a sample cell for observation use, which makes it possible to observe three-dimensionally cultured cells from various angles. The cell culture vessel or the sample cell for observation use according to the present invention is characterized by being equipped with a culturing gel in which cells or cell tissues are embedded and a first vessel which encloses the culturing gel, wherein the space in the first vessel is filled with the culturing gel and the first vessel has a light-permeable window made from a hydrogel.
Scope of claims [claim1]
1. The 1st container which contains the culture gel and the aforementioned culture gel which embed cell or tissue having,
As for the aforementioned culture gel, satisfying space inside 1st container,
As for the 1st container, the cell culture container which features that it possesses the translucent window which consists of haidorogeru.
[claim2]
2. As for the aforementioned window, agarosugeru and the poly- acrylamide gel, in the claim 1 which includes the alginic acid sodium or the collagen gel the cell culture container of statement.
[claim3]
3. As for the aforementioned window, 50g/cm (2) above 10000g/cm (2) in the claim 1 which possesses the gel strength below or 2 the cell culture container of statement.
[claim4]
4. Form of the 1st container is cube or the rectangular parallelepiped,
As for the 1st container, either of the claim 1-3 which possesses the aforementioned window on each surface in one the cell culture container of statement.
[claim5]
5. As for the 1st container, either of the claim 1-4 which possesses the framework section which surrounds the aforementioned window in one the cell culture container of statement.
[claim6]
6. The 2nd container which accumulates the liquid nutrient medium furthermore having,
As for the 1st container, either of the claim 1-5 which is arranged in the aforementioned liquid nutrient medium in one the cell culture container of statement.
[claim7]
7. The 1st container which contains the culture gel and the aforementioned culture gel which embed cell or tissue having,
As for the aforementioned culture gel, satisfying space inside 1st container,
As for the 1st container, the sample cell for observation which features that it possesses the translucent window which consists of haidorogeru.
[claim8]
8. Inside the culture gel which embeds cell or tissue fills up having the 1st container which possesses the space,
As for the 1st container, the cell culture container which features that it possesses the translucent window which consists of haidorogeru.
[claim9]
9. Form of the 1st container is cube or the rectangular parallelepiped,
As for the 1st container, in the claim 8 which possesses the aforementioned window on each side the cell culture container of statement.
[claim10]
10. From opening of the 1st container which possesses the translucent window which consists of haidorogeru in the internal space of the 1st container the culture gel and production method of the cell culture container which has the process which fills up cell or tissue.
[claim11]
11. The aforementioned internal space the culture gel and after filling up with cell or tissue, in the claim 10 which has the process which forms the translucent window which consists of haidorogeru in the aforementioned opening production method of statement.
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • OSAKA PREFECTURE UNIVERSITY
  • KYUSHU INSTITUTE OF TECHNOLOGY
  • Inventor
  • HAGIWARA MASAYA
  • KAWAHARA TOMOHIRO
IPC(International Patent Classification)
Specified countries (WO201794451)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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