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GENETIC MODIFICATION NON-HUMAN ORGANISM, EGG CELLS, FERTILIZED EGGS, AND METHOD FOR MODIFYING TARGET GENES

外国特許コード F170009118
整理番号 (AF37P001)
掲載日 2017年7月7日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP085391
国際公開番号 WO 2017104404
国際出願日 平成28年11月29日(2016.11.29)
国際公開日 平成29年6月22日(2017.6.22)
優先権データ
  • 特願2015-247960 (2015.12.18) JP
発明の名称 (英語) GENETIC MODIFICATION NON-HUMAN ORGANISM, EGG CELLS, FERTILIZED EGGS, AND METHOD FOR MODIFYING TARGET GENES
発明の概要(英語) The present invention provides a genetic modification non-human organism where Cas9 expression is higher and a plurality of different genes, or a plurality of sites within the same gene, can be simultaneously edited with high efficiency. This genetic modification non-human organism has at least three copies of the gene encoding Cas9 (CRISPR-associated 9) within the nuclear genome. These egg cells are derived from a genetic modification non-human organism having a nuclear genome into which at least three copies of a gene encoding CAS9 have been introduced. These fertilized eggs are obtained by allowing fertilization to occur between the egg cells and sperm derived from an organism of the same species. This method for modifying a target gene comprises a step for introducing guide RNA to cells derived from the genetic modification non-human organism, the egg cells, or the fertilized eggs.
従来技術、競合技術の概要(英語) BACKGROUND ART
The development of new drugs or the study of the mechanisms of the disease in the studies, reflecting the pathology of the disease the presence of disease model animals, holds a key to success or failure of the study. With respect to genetic factors of human disease, by the recent rapid development of technology for genome analysis, SNP locus spanning millions of locations and the genetic information can be obtained relatively easily as compared to the case. For numerous disorders using this technique, a large number of diseases associated with genetic loci have been identified, reported. On the other hand, such the genetically modified mouse of disease candidate genes may be prepared, in most cases do not exhibit the phenotype. This is, unusual for a single gene, such as life-style related diseases can be explained a disease involving a multi-factor is not certain. Therefore, each of these candidate genes for drug discovery that target considered to be a limit in the effect. To solve such problem, quickly multilocus modified disease model animals, prepared and systematically, but necessary to adjust a regime utilized, conventional ES cell that genetic modification method, operation 1, locus 1, cannot be genetically modified in the two places of 1 only, it is difficult to realize this.
In recent years, genetically modified CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system with the advent of the technology. CRISPR system, through the genetic modification of the conventional ES cells having a potential for a novel transcendent techniques. CRISPR system, 3 types of RNA or DNA, a guide RNA of the target gene that is cut, the cutting device is engaged in a nuclease Cas9(CRISPR-associated 9) mRNA of, a gene region that cleavage of the gene is a modified integrated homologously into a fertilized egg to the microscopic injection type 3, by generating the embryonic to adulthood, the genetically modified mouse is obtained.
The non-patent document 1, the system can be used CRISPR, ES cells of the ROSA26 gene encoding the Cas9 gene region by knock-in, can manufacture knock-in mice Cas9 described.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • SHINDO Takayuki
  • SAKURAI Takayuki
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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