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TARGET ANALYSIS METHOD AND TARGET ANALYSIS CHIP

Foreign code F170009141
File No. (S2016-0285-N0)
Posted date Aug 8, 2017
Country WIPO
International application number 2017JP001280
International publication number WO 2017126479
Date of international filing Jan 16, 2017
Date of international publication Jul 27, 2017
Priority data
  • P2016-011018 (Jan 22, 2016) JP
Title TARGET ANALYSIS METHOD AND TARGET ANALYSIS CHIP
Abstract Provided are a novel target analysis method and target analysis chip for directly detecting targets such as micro-RNA without performing PCR. This target analysis method is characterized in comprising: a step for bringing a target in a sample, a labelling probe for binding to the target, and a carrier-binding probe for binding to the target and a carrier into contact to cause a binding reaction between the target, the labelling probe and the carrier-binding probe and form a first complex; a step for bringing the first complex into contact with the carrier to cause a binding reaction between the first complex and the carrier and form a second complex; a step for concentrating the carrier; and a step for analyzing the target in the sample by detecting the labelling of the labelling probe that is bound to the concentrated carrier.
Scope of claims [claim1]
1. The 1st reaction process which is the process where it contacts with the sign probe which is connected to the target and the aforementioned target in the sample and the probe for carrier connection which is connected to the carrier which was formed with the aforementioned target and the material which possesses optical permeability in the liquid solvent, the aforementioned target and the aforementioned sign probe and the probe for the aforementioned carrier connection it connects reacts, forms 1st joining union,
The description above 1st it contacts with joining union and the aforementioned carrier, the description above 1st joining union and the aforementioned carrier it connects reacts, the 2nd reaction process which is the process which forms the 2nd joining union whose relative importance is larger than the aforementioned liquid solvent,
The concentrated process which is the process which centrifugal does the aforementioned liquid solvent which includes the aforementioned 2nd joining union, concentrates the aforementioned 2nd joining union, and
The description above when it is concentrated, during the aforementioned 2nd joining uniting it features that the analysis process which is the process which analyzes the target in the aforementioned sample by detecting the sign of the aforementioned sign probe which is included, is included, analysis method of the target.
[claim2]
2. Probe for the aforementioned carrier connection, implication 1st cementing material,
The aforementioned carrier, implication the 2nd cementing material which is connected with the aforementioned 1st cementing material,
The probe for the aforementioned carrier connection, through the connection with the aforementioned 1st cementing material and the aforementioned 2nd cementing material, it connects with the aforementioned carrier, analysis method of the target of claim 1 statement.
[claim3]
3. The aforementioned target, is target nuclear acid, claim analysis method of the target of 1 or 2 statements.
[claim4]
4. The aforementioned target nuclear acid, is micro RNA, analysis method of the target of claim 3 statement.
[claim5]
5. The aforementioned sign probe, is the fluorescent sign probe, claim analysis method of the target of 3 or 4 statements.
[claim6]
6. The aforementioned fluorescent sign probe, by comparison with the probe for the aforementioned carrier connection, connects 5' on edge side of the aforementioned target nuclear acid, analysis method of the target of claim 5 statement.
[claim7]
7. The aforementioned fluorescent sign probe, connects to the base which is continued 5' from the edge of the aforementioned target nuclear acid,
The probe for the aforementioned carrier connection, connects to the base which is continued 3' from the edge of the aforementioned target nuclear acid, analysis method of the target of claim 6 statement.
[claim8]
8. The fluorescent sign of the aforementioned fluorescent sign probe, the signal is the substance which changes due to the fact that the aforementioned fluorescent sign probe connects to the aforementioned target nuclear acid, from claim 5 either up to 7 in one section analysis method of the target of statement.
[claim9]
9. The aforementioned fluorescent sign, is the substance which includes pyrene, analysis method of the target of claim 8 statement.
[claim10]
10. The aforementioned sign probe, furthermore is decorated with soraren,
The process which makes with aforementioned soraren and the aforementioned target nuclear acid connect by producing the ultraviolet ray, is included, from claim 3 either up to 9 in one section analysis method of the target of statement.
[claim11]
11. The reaction section, the target analysis tip/chip which possesses the baseplate which has the passage which communicates with the sensing station, and the aforementioned reaction section and the aforementioned sensing station is used,
The aforementioned reaction section the aforementioned target, is the region where the aforementioned sign probe, the probe and the aforementioned carrier for the aforementioned carrier connection are bonded react,
The aforementioned sensing station is the region where the aforementioned 2nd joining union is concentrated,
As for the aforementioned passage, possessing the hydrophobic inner wall as the portable control means which control the movement to the aforementioned sensing station from the aforementioned reaction section of the aforementioned 2nd joining union,
Due to the aforementioned portable control means, the description above 2nd when the resistivity which it occurs with the hydrophobia of the aforementioned passage (R) centrifugal force it is larger (C) it is loaded, through the aforementioned passage, be able to move joining union, to the aforementioned sensing station,
Includes the aforementioned target in the aforementioned reaction section after the sample which is introduced, at the aforementioned reaction section the aforementioned 1st reaction process the action,
Furthermore at the aforementioned reaction section the aforementioned 2nd reaction process action,
Through the aforementioned passage from the aforementioned reaction section, while reaching to the aforementioned sensing station, aforementioned concentrated process the action,
In the aforementioned sensing station, the aforementioned analysis process is done, from claim 1 either up to 10 in one section analysis method of the target of statement.
[claim12]
12. Height of the aforementioned sensing station, is 1-500 .micro.m, analysis method of the target of claim 11 statement.
[claim13]
13. The aforementioned carrier, is the beads, from claim 1 either up to 12 in one section analysis method of the target of statement.
[claim14]
14. Diameter of the aforementioned beads, is 100nm-4 .micro.m, analysis method of the target of claim 13 statement.
[claim15]
15. The reaction section, possessing the baseplate which has the passage which communicates with the sensing station, and the aforementioned reaction section and the aforementioned sensing station,
The aforementioned reaction section the sign probe which is connected to the target and the aforementioned target in the sample, is the region where the probe and the aforementioned carrier which for carrier connection are connected to the carrier which was formed with the aforementioned target and the material which possesses optical permeability are bonded react,
The aforementioned sensing station, the aforementioned target, through 1st joining union and the probe for the aforementioned carrier connection which the aforementioned sign probe and the probe for the aforementioned carrier connection are bonded, is the region where the 2nd joining union which was formed due to the fact that connects with the aforementioned carrier, is concentrated,
As for the aforementioned passage, possessing the hydrophobic inner wall as the portable control means which control the movement to the aforementioned sensing station from the aforementioned reaction section of the aforementioned 2nd joining union,
With the aforementioned portable control means, in the liquid solvent, the description above whose relative importance is larger than the aforementioned liquid solvent 2nd joining union when the resistivity which it occurs with the hydrophobia of the aforementioned passage (R) centrifugal force it is larger (C) it is loaded, through the aforementioned passage, features that it can move to the aforementioned sensing station, the analysis tip/chip of the target.
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • ARKRAY, INC.
  • Kyoto Institute of Technology
  • Inventor
  • MURAKAMI AKIRA
  • KOBORI AKIO
  • YAMAYOSHI ASAKO
  • NODA YUICHIRO
IPC(International Patent Classification)
Specified countries (WO2017126479)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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