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VITRIFICATION STABILIZER FOR ANIMAL CELL CRYOPRESERVATION FLUID

外国特許コード F170009159
整理番号 (S2016-0438-N0)
掲載日 2017年8月29日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2017JP005592
国際公開番号 WO 2017141991
国際出願日 平成29年2月15日(2017.2.15)
国際公開日 平成29年8月24日(2017.8.24)
優先権データ
  • 特願2016-027612 (2016.2.17) JP
発明の名称 (英語) VITRIFICATION STABILIZER FOR ANIMAL CELL CRYOPRESERVATION FLUID
発明の概要(英語) Provided are a vitrification stabilizer for an animal cell cryopreservation fluid, and an animal cell cryopreservation fluid which exhibits superior vitrification capabilities due to the animal cell cryopreservation fluid containing the vitrification stabilizer for an animal cell cryopreservation fluid. The vitrification stabilizer for an animal cell cryopreservation fluid contains: an amphoteric polymer compound selected from the group consisting of (a) a carboxylated amphoteric polymer compound obtained by reacting ε-poly-L-lysine with butyl succinic anhydride, (b) a carboxylated amphoteric polymer compound obtained by reacting ε-poly-L-lysine with butyl succinic anhydride and succinic anhydride, or (c) a carboxylated amphoteric polymer compound obtained by reacting ε-poly-L-lysine with a compound represented by formula I; and (d) a sucrose polymer macromolecule to which epichlorohydrin has been crosslinked.
従来技術、競合技術の概要(英語) BACKGROUND ART
In recent years, clinical application of regenerative medicine is highly expected to. As the basic technique for this purpose, cells, cell sheets, three dimensional shape of the cell constructs, tissue, and stored frozen and there is a need. Such a technique, freezing protection substance is added, while preventing damage to the object, a technique of cryopreservation. Such as a cryo-protectant, dimethyl sulfoxide (DMSO) (O=S (CH3)2) 、known glycerin and the like.
DMSO or glycerol and is, in a cell dispersed for use in cryopreservation, sufficient is the same. However, is DMSO or glycerol, a cell sheet, cell constructs three-dimensional shape, such as in use with the tissue for cryopreservation, often the damage state after thawing occurs, the damage being left. This damage, ice crystals formed during freezing and dehydration considered to arise from contraction to, control of ice crystallization, amorphous in the state of a hardened, vitrification method, have been tried.
Vitrification is, frozen fertilized eggs and has been developed as a method, the conventional vitrification is, in general, by rapid freezing rate and a high solute concentration, an aqueous solution intended to be vitrified. For example, as a solution into the glass of the fertilized egg of the mouse, DAP213 is known. Is DAP213, DMSO 2M, 1M acetamide, a solution of propylene glycol and 3M, with a high concentration, high toxicity. A typical use method, a mouse fertilized egg contained in the water which, in this with solution, and then immersed in a liquid directly to the nitrogen, to obtain an glass state. However, such a vitrification solution is introduced into the glass, cells and high toxicity, damage caused by further recrystalization upon thawing is also high.
Therefore, the present inventors, considering the cryo-protectant material, to improve the method of the have attempted (patent document 1).
Is Patent Document 2, a large amount of sucrose is the polymer preparation.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY
  • SHIBUYA CORPORATION
  • 発明者(英語)
  • MATSUMURA,Kazuaki
  • NAKA,Toshiaki
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
※ 上記の特許・技術に関心のある方は、下記問合せ先にご相談下さい。

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