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Nucleic acid-encapsulating polymer micelle complex and method for producing same
Nucleic acid-encapsulating polymer micelle complex and method for producing same
外国特許コード
F170009167
整理番号
K10503WO
掲載日
2017年9月12日
出願国
オーストラリア
出願番号
2014303571
公報番号
2014303571
公報番号
2014303571
出願日
平成26年8月5日(2014.8.5)
公報発行日
平成28年2月18日(2016.2.18)
公報発行日
平成29年3月2日(2017.3.2)
国際出願番号
JP2014070567
国際公開番号
WO2015020026
国際出願日
平成26年8月5日(2014.8.5)
国際公開日
平成27年2月12日(2015.2.12)
優先権データ
特願2013-163106 (2013.8.6) JP
2014WO-JP70567 (2014.8.5) WO
発明の名称 (英語)
Nucleic acid-encapsulating polymer micelle complex and method for producing same
発明の概要(英語)
(AU2014303571)
This nucleic acid-encapsulating polymer micelle complex is characterized in being formed of: a block copolymer containing an uncharged hydrophilic polymer chain block and a cationic polymer chain block; and two single-stranded DNA molecules comprising mutually complementary base sequences of 1000 or more bases in length, double-stranded DNA of 1000 or more base pairs in length in which at least a part of the double helix structure has dissociated and taken on a single-stranded structure, or one single-stranded DNA molecule of 1000 or more bases in length.
特許請求の範囲(英語)
[claim1]
1. A nucleic acid-encapsulating polymer micelle complex formed of a block copolymer containing an uncharged hydrophilic polymer chain block and a cationic polymer chain block; and two single-stranded DNAs having mutually complementary base sequences of 1000 or more bases in length, double-stranded DNA of 1000 or more base pairs in length in which at least a part of a double helix structure is dissociated and forms a single-stranded structure, or one single-stranded DNA of 1000 or more bases in length.
[claim2]
1.
[claim3]
2. The nucleic acid-encapsulating polymer micelle complex according to Claim 1, formed of a block copolymer containing an uncharged hydrophilic chain block and a cationic polymer chain block; and two single-stranded DNAs having mutually complementary base sequences of 1000 or more bases in length or double-stranded DNA of 1000 or more base pairs in length in which at least a part of a double helix structure is dissociated and forms a single-stranded structure.
[claim4]
3. The nucleic acid-encapsulating polymer micelle complex according to Claim 1 or 2, wherein the single-stranded DNA is 2000 or more bases in length, and the double-stranded DNA is 2000 or more base pairs in length.
[claim5]
4. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 3, wherein the average particle diameter thereof in an aqueous medium measured according to a dynamic light scattering method is 100 nm or less.
[claim6]
5. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 4, wherein the DNA and the cationic polymer chain block bonded to the DNA due to an electrostatic interaction form a core portion, and the uncharged hydrophilic polymer chain block forms a shell portion.
[claim7]
6. The nucleic acid-encapsulating polymer micelle complex according to Claim 5, wherein the average particle diameter of the core portion is 50 nm or less.
[claim8]
7. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 6, wherein the complex is spherical.
[claim9]
8. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 7, wherein the single-stranded DNA or the double-stranded DNA is linear.
[claim10]
9. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 8, wherein at least a part of the block copolymer is mutually cross-linked.
[claim11]
10. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 9, wherein a hydrophobic group is covalently bonded to a main chain or a side chain of the cationic polymer chain block.
[claim12]
11. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 10, wherein the cationic polymer chain block has an ethylamine structure or a propylamine structure in the side chain thereof.
[claim13]
12. A method for producing a nucleic acid-encapsulating polymer micelle complex which accommodates DNA, the method comprising:
a process of mixing a block copolymer containing an uncharged hydrophilic polymer chain block and a cationic polymer chain block with double-stranded DNA of 1000 or more base pairs in a state in which at least a part of a double helix structure is dissociated, in an aqueous medium.
[claim14]
13. The method for producing a nucleic acid-encapsulating polymer micelle complex according to Claim 12, wherein the double-stranded DNA is 2000 or more base pairs in length.
[claim15]
14. The method for producing a nucleic acid-encapsulating polymer micelle complex according to Claim 12 or 13, wherein the double-stranded DNA is linear.
[claim16]
15. The method for producing a nucleic acid-encapsulating polymer micelle complex according to any one of Claims 12 to 14, wherein the double-stranded DNA has been denatured at 60'C or higher.
出願人(英語)
JAPAN SCIENCE AND TECHNOLOGY AGENCY
発明者(英語)
KATAOKA KAZUNORI
OSADA KENSUKE
TOCKARY THEOFILUS AGRIOS
国際特許分類(IPC)
A61K 9/107
エマルジョン
A61K 31/711
天然のデオキシリボ核酸,すなわちアデニン,グアニン,シトシン又はチミンに結合した2’-デオキシリボースのみを含み,3’-5’ホスホジエステル結合を持つもの
A61K 31/713
2本鎖の核酸またはオリゴヌクレオチド
A61K 47/34
炭素-炭素不飽和結合のみが関与する反応以外の反応によって得られる高分子化合物,例.ポリエステル,ポリアミノ酸,ポリシロキサン,ポリホスファゼン,ポリアルキレングリコールの共重合体またはポロキサマーの共重合体
A61K 48/00
遺伝子疾病を治療するために生体の細胞内に挿入する遺伝子物質を含有する医療用製剤;遺伝子治療
C12N 15/115
アプタマー、すなわち、ハイブリダイズ以外の手段で、特異的にかつ高親和性で標的分子に結合する核酸
参考情報 (研究プロジェクト等)
PRESTO Molecular technology and creation of new functions AREA
日本語項目の表示
発明の名称
核酸内包高分子ミセル複合体及びその製造方法
※
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『 Nucleic acid-encapsulating polymer micelle complex and method for producing same 』に関するお問合せ
国立研究開発法人科学技術振興機構(JST) 知的財産マネジメント推進部
URL:
http://www.jst.go.jp/chizai/
E-mail:
Address: 〒102-8666 東京都千代田区四番町5-3
TEL: 03-5214-8293
FAX: 03-5214-8476
関連情報
公報
2014303571(PDF,3344KB)
公報
2014303571(PDF,3778KB)