Nucleic acid-encapsulating polymer micelle complex and method for producing same
| 外国特許コード | F170009167 |
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| 整理番号 | K10503WO |
| 掲載日 | 2017年9月12日 |
| 出願国 | オーストラリア |
| 出願番号 | 2014303571 |
| 公報番号 | 2014303571 |
| 公報番号 | 2014303571 |
| 出願日 | 平成26年8月5日(2014.8.5) |
| 公報発行日 | 平成28年2月18日(2016.2.18) |
| 公報発行日 | 平成29年3月2日(2017.3.2) |
| 国際出願番号 | JP2014070567 |
| 国際公開番号 | WO2015020026 |
| 国際出願日 | 平成26年8月5日(2014.8.5) |
| 国際公開日 | 平成27年2月12日(2015.2.12) |
| 優先権データ |
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| 発明の名称 （英語） | Nucleic acid-encapsulating polymer micelle complex and method for producing same |
| 発明の概要（英語） |
(AU2014303571) This nucleic acid-encapsulating polymer micelle complex is characterized in being formed of: a block copolymer containing an uncharged hydrophilic polymer chain block and a cationic polymer chain block; and two single-stranded DNA molecules comprising mutually complementary base sequences of 1000 or more bases in length, double-stranded DNA of 1000 or more base pairs in length in which at least a part of the double helix structure has dissociated and taken on a single-stranded structure, or one single-stranded DNA molecule of 1000 or more bases in length. |
| 特許請求の範囲（英語） |
[claim1] 1. A nucleic acid-encapsulating polymer micelle complex formed of a block copolymer containing an uncharged hydrophilic polymer chain block and a cationic polymer chain block; and two single-stranded DNAs having mutually complementary base sequences of 1000 or more bases in length, double-stranded DNA of 1000 or more base pairs in length in which at least a part of a double helix structure is dissociated and forms a single-stranded structure, or one single-stranded DNA of 1000 or more bases in length. [claim2] 1. [claim3] 2. The nucleic acid-encapsulating polymer micelle complex according to Claim 1, formed of a block copolymer containing an uncharged hydrophilic chain block and a cationic polymer chain block; and two single-stranded DNAs having mutually complementary base sequences of 1000 or more bases in length or double-stranded DNA of 1000 or more base pairs in length in which at least a part of a double helix structure is dissociated and forms a single-stranded structure. [claim4] 3. The nucleic acid-encapsulating polymer micelle complex according to Claim 1 or 2, wherein the single-stranded DNA is 2000 or more bases in length, and the double-stranded DNA is 2000 or more base pairs in length. [claim5] 4. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 3, wherein the average particle diameter thereof in an aqueous medium measured according to a dynamic light scattering method is 100 nm or less. [claim6] 5. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 4, wherein the DNA and the cationic polymer chain block bonded to the DNA due to an electrostatic interaction form a core portion, and the uncharged hydrophilic polymer chain block forms a shell portion. [claim7] 6. The nucleic acid-encapsulating polymer micelle complex according to Claim 5, wherein the average particle diameter of the core portion is 50 nm or less. [claim8] 7. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 6, wherein the complex is spherical. [claim9] 8. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 7, wherein the single-stranded DNA or the double-stranded DNA is linear. [claim10] 9. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 8, wherein at least a part of the block copolymer is mutually cross-linked. [claim11] 10. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 9, wherein a hydrophobic group is covalently bonded to a main chain or a side chain of the cationic polymer chain block. [claim12] 11. The nucleic acid-encapsulating polymer micelle complex according to any one of Claims 1 to 10, wherein the cationic polymer chain block has an ethylamine structure or a propylamine structure in the side chain thereof. [claim13] 12. A method for producing a nucleic acid-encapsulating polymer micelle complex which accommodates DNA, the method comprising: a process of mixing a block copolymer containing an uncharged hydrophilic polymer chain block and a cationic polymer chain block with double-stranded DNA of 1000 or more base pairs in a state in which at least a part of a double helix structure is dissociated, in an aqueous medium. [claim14] 13. The method for producing a nucleic acid-encapsulating polymer micelle complex according to Claim 12, wherein the double-stranded DNA is 2000 or more base pairs in length. [claim15] 14. The method for producing a nucleic acid-encapsulating polymer micelle complex according to Claim 12 or 13, wherein the double-stranded DNA is linear. [claim16] 15. The method for producing a nucleic acid-encapsulating polymer micelle complex according to any one of Claims 12 to 14, wherein the double-stranded DNA has been denatured at 60'C or higher. |
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| 国際特許分類(IPC) |
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| 参考情報 （研究プロジェクト等） | PRESTO Molecular technology and creation of new functions AREA |
日本語項目の表示
| 発明の名称 | 核酸内包高分子ミセル複合体及びその製造方法 |
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