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Nucleic acid-encapsulating polymer micelle complex and method for producing same UPDATE

外国特許コード F170009170
整理番号 K10503WO
掲載日 2017年9月12日
出願国 大韓民国
出願番号 20167001466
公報番号 20160021291
公報番号 101770705
出願日 平成26年8月5日(2014.8.5)
公報発行日 平成28年2月24日(2016.2.24)
公報発行日 平成29年8月23日(2017.8.23)
国際出願番号 JP2014070567
国際公開番号 WO2015020026
国際出願日 平成26年8月5日(2014.8.5)
国際公開日 平成27年2月12日(2015.2.12)
優先権データ
  • 特願2013-163106 (2013.8.6) JP
  • 2014JP70567 (2014.8.5) WO
発明の名称 (英語) Nucleic acid-encapsulating polymer micelle complex and method for producing same UPDATE
発明の概要(英語) A nucleic acid-encapsulating polymer micelle complex is formed of a block copolymer containing an uncharged hydrophilic polymer chain block and a cationic polymer chain block; and two single-stranded DNA molecules having mutually complementary base sequences of 1000 or more bases in length, double-stranded DNA of 1000 or more base pairs in length in which at least a part of a double helix structure is dissociated and forms a single-stranded structure, or one single-stranded DNA molecule of 1000 or more bases in length.
従来技術、競合技術の概要(英語) BACKGROUND ART
As a next generation method for the treatment of a disease by controlling gene expression in gene therapy a therapeutically effective amount of molybdenum is awaited in great expectation for the present invention. The maximum object of gene therapy to target cells or tissue of a gene is introduced into the introduction efficiency is insufficient due to the fact that when the present invention. In particular, by the systemic administration of gene therapy methods in order to achieve a circulating blood gene is stably integrated in the target tissue which, after entering the target cell may also effectively gene expression is to be performed is required. Thus, in order to solve these problems to a target cell or target cell transduction efficiency and the like in a better efficiency of gene expression (gene carrier) of a gene carrier is actively developed.
For example, 1 to precisely control the primary structure of the polymer to generate spontaneously organized micelle, vesicle etc of the higher order structure can be formed and it is known, such a polymer structure which can be used as a self or a drug delivery system of material Science in various fields including electric field has been studied. For example, patent document 1 includes a conductive segment (conductive polymer chain block than) as compared with the charged segment (charged polymer chain block) composed of a block copolymer having, as opposed to the core portion of the charged peptide segment contains at least one drug having a negatively charged electrostatic polymer micelle can be carrier agents have been disclosed. The charged peptide segment by using a cationic segment as a core portion containing DNA may also be useful.
In addition, to increase the stability of polymeric micelles than designed various also has been reported. For example, patent document 2 electrostatic bond type polymeric micelle drug carrier through the crosslinking agent to crosslink the block copolymers to stabilize the coalesce in the electrostatic bond type polymeric micelle drug carriers is disclosed. In addition, patent document 3 is provided with a hydrophilic polymer chain block and a conductive over, a portion of the side chain of the hydrophobic group is introduced by cationic polyamino shrub block comprising a block copolymer is disclosed in Fig. The hydrophobic group is introduced into the side chain of the block copolymer can be obtained by the interfacial energy increases because the cohesive force within the core of the micelles is enhanced which decreases as a result, the stability of the polymeric micelles has been achieved.
特許請求の範囲(英語) [claim1]
1. An uncharged hydrophilic polymer chain block and a cationic polymer chain comprising a block copolymer and a block, at least 1000 bases in length consisting of a nucleotide sequence complementary to the other of the two DNA 2 chain 1, 2 is dissociated in a portion of the spiral structure 1 wherein the chain structure of at least 1000 base pairs in length of 1000 bases from the 2 1 chain of two or more DNA chain consisting of 1 and formed as a DNA, wherein the specific nucleic acid in the shape of a polymer micelle complexes therein.

[claim2]
2. Method according to claim 1, an uncharged hydrophilic polymer chain block and a cationic polymer chain comprising a block copolymer comprising blocks, at least 1000 bases which are complementary to the base sequence of 2 2 or 1 in the chain portion of the spiral structure DNA dissociated 1 wherein the chain structure of at least 1000 base pairs in length as described in claim 2 formed into a chain DNA the nucleic acid polymer micelle complexes therein.

[claim3]
3. Method according to claim 1 or 2, wherein the chain length of at least 1 2000 bases and is DNA, wherein the chain length of at least 2000 base pairs is 2 DNA wherein the polymer micelle complex containing a nucleic acid.

[claim4]
4. Method according to claim 1 or 2, in an aqueous medium by a dynamic light scattering of 100nm or less and the average particle diameter of the polymer micelle complex containing a nucleic acid.

[claim5]
5. Method according to claim 1 or 2, DNA and, based on the electrostatic interaction of the cationic polymer chain DNA bound to form a core portion, an uncharged hydrophilic polymer chain block is in the form of a shell portion of said polymer micelle complex nucleic acid therein.

[claim6]
6. Method according to claim 5, wherein the average particle diameter of the core portion is not less than 50nm of the nucleic acid polymer micelle complexes therein.

[claim7]
7. AMEND STATUS: Deleted

[claim8]
8. Method according to claim 1 or 2, wherein the chain 2 or chain 1 is a linear DNA DNA wherein said polymeric micelle complex nucleic acid therein.

[claim9]
9. Method according to claim 1 or 2, wherein a portion of the block copolymers are crosslinked polymer micelle complex containing the nucleic acid.

[claim10]
10. Method according to claim 1 or 2, wherein the cationic polymer chain block is covalently bonded to the hydrophobic group in the main chain or the nucleic acid and a polymeric micellar complex therein.

[claim11]
11. Method according to claim 1 or 2, wherein the cationic polymer in the side chain amine of the amine structure or a block polymer having a structure containing polymeric micelle complex of the nucleic acid.

[claim12]
12. Receiving a polymer micelle DNA complex of a nucleic acid as a method of containing, an uncharged hydrophilic polymer chain block and a cationic polymer chain comprising a block copolymer comprising blocks, after dissociation of the part of the structure 2 in a state of a chain of at least 1000 base pairs 2 DNA mixing in an aqueous medium and, wherein the nucleic acid in a polymer micelle complex containing the nucleic acid in the shape of a method for the preparation of a polymer micelle complexes therein.

[claim13]
13. Method according to claim 12, wherein the chain length of 2 base pairs is at least 2000 DNA is characterized in that the method of preparing a polymer micelle complex containing a nucleic acid.

[claim14]
14. Method according to claim 12 or 13, wherein 2 is a linear chain DNA is a method of manufacturing a polymer micelle complex containing a nucleic acid.

[claim15]
15. Method according to claim 12 or 13, wherein at least 60°C modified DNA is 2 in chain and a method of manufacturing a nucleic acid polymer micelle complexes therein.
  • 出願人(英語)
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • KATAOKA KAZUNORI
  • OSADA KENSUKE
  • TOCKARY THEOFILUS AGRIOS
国際特許分類(IPC)
参考情報 (研究プロジェクト等) PRESTO Molecular technology and creation of new functions AREA
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