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Formation and use of neuronal network, and neuron seeding device UPDATE

外国特許コード F170009187
整理番号 AF19-04WO
掲載日 2017年9月12日
出願国 アメリカ合衆国
出願番号 201314429035
公報番号 20150233890
公報番号 9829477
出願日 平成25年3月21日(2013.3.21)
公報発行日 平成27年8月20日(2015.8.20)
公報発行日 平成29年11月28日(2017.11.28)
国際出願番号 JP2013057976
国際公開番号 WO2014045618
国際出願日 平成25年3月21日(2013.3.21)
国際公開日 平成26年3月27日(2014.3.27)
優先権データ
  • 特願2012-205561 (2012.9.19) JP
  • 2013WO-JP57976 (2013.3.21) WO
発明の名称 (英語) Formation and use of neuronal network, and neuron seeding device UPDATE
発明の概要(英語) (US9829477)
A planar patch clamp device is disclosed, which can be used for culturing a neuron in the device so as to form a neuron network, and detecting an electrical property of the neuron that forms the neuron network.
The planar patch clamp device includes a plurality of protrusions formed on a first surface, an extracellular matrix forming substance which is coated on the peripheries of a through hole, and electrode sections.
特許請求の範囲(英語) [claim1]
1. A planar patch clamp device for analyzing a neuron network, comprising: an electrical insulating plate having a first surface, on which a neuron network is formed, a second surface, which is the opposite side, and a through-hole having a diameter which allows passing through both sides of the plate surface, but does not allow the passage of a cell body;
a first liquid pool section formed on the first surface, which enables holding a conductive solution;
a second liquid pool section formed on the second surface, which enables holding a pipet solution, and is communicated with the first liquid pool through the through-hole;
a first surface side-electrode disposed to be electrically conductive to the conductive solution; and
a second surface side-electrode disposed to be electrically conductive to the pipet solution;
wherein a cell plating section surrounded by a plurality of protrusions is formed on the first surface so that the protrusions restrict the movement of cells, but allow the formation of a neuron network, the through-hole is arranged in the cell plating section, and the peripheries of the through-hole are coated with an extracellular matrix-forming substance, and
wherein each of the electrodes on the first surface side and second surface side comprise:
(a) an electrode receptacle for holding each of the electrodes, wherein at least a portion of the receptacle wall that is in contact with the conductive solution or the pipet solution, when the conductive solution or the pipet solution is introduced into the liquid pool sections, is composed of an inorganic porous material;
(b) each of the electrodes having a precious metal chloride (NmCl) layer formed on a surface layer section of the precious metal (Nm), and housed in the electrode receptacle; and
(c) an electrode solution filled into the electrode receptacle, wherein the precious metal chloride (NmCl) and an alkali metal chloride are dissolved at saturated concentration; and
wherein when cells are plated on the cell plating section, both of the first and second surface-side electrodes are not in contact with a cell body.
[claim2]
2. The planar patch clamp device for analyzing a neuron network according to claim 1, wherein the diameter of the cell plating section defined by the plurality of protrusions is a size that enables accommodating one to several cell bodies.
[claim3]
3. The planar patch clamp device for analyzing a neuron network according to claim 1, wherein the plurality of cell plating sections defined by the plurality of protrusions are arranged on the plate.
[claim4]
4. The planar patch clamp device for analyzing a neuron network according to claim 1, one to several cell bodies are placed in the cell placing section, and another cell body is placed outside of the cell placing section.
[claim5]
5. The planar patch clamp device for analyzing a neuron network according to claim 1, characterized in that the device is used for measurement and analysis of neuronal ion channel current or for imaging analysis.
[claim6]
6. The planar patch clamp device for analyzing a neuron network according to claim 1 further comprising: a photodetector for detecting light emitted by neurons; and
an irradiating device that irradiates light onto the neurons or plate surface.
  • 発明者/出願人(英語)
  • URISU TSUNEO
  • WANG ZHI-HONG
  • UNO HIDETAKA
  • SAITOH MIHO
  • NAGAOKA YASUTAKA
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
参考情報 (研究プロジェクト等) CREST Creation of Nanosystems with Novel Functions through Process Integration AREA
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