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Planar patch clamp device and planar patch clamp system UPDATE

外国特許コード F170009188
整理番号 AF19-07WO
掲載日 2017年9月12日
出願国 アメリカ合衆国
出願番号 201414915560
公報番号 20160209395
公報番号 9977009
出願日 平成26年8月29日(2014.8.29)
公報発行日 平成28年7月21日(2016.7.21)
公報発行日 平成30年5月22日(2018.5.22)
国際出願番号 JP2014072808
国際公開番号 WO2015030201
国際出願日 平成26年8月29日(2014.8.29)
国際公開日 平成27年3月5日(2015.3.5)
優先権データ
  • 特願2013-180684 (2013.8.30) JP
  • 2014WO-JP72808 (2014.8.29) WO
発明の名称 (英語) Planar patch clamp device and planar patch clamp system UPDATE
発明の概要(英語) (US9977009)
a second reservoir (6′) provided at the second surface side (2S′) of the electrical insulating substrate to be able to communicate with the through hole (3) and hold a second conductive liquid; a second electrode part (7′) arranged to be able to be electrically conductive with the second reservoir (6′) through the second conductive liquid; a supply path (8) connected to the second reservoir (6′) and supplying the second conductive liquid to the second reservoir; a discharge path (9) connected to the second reservoir and discharging the second conductive liquid from the second reservoir (6′); and a valve (10) provided in the supply path and/or discharge path, able to allow or stop the flow of the second conductive liquid, and also able to allow or stop electrical conduction between the second reservoir (6′) and the second electrode part (7′).
特許請求の範囲(英語) [claim1]
1. A planar patch clamp device comprising: a first reservoir able to hold a first conductive liquid;
a first electrode part arranged to be able to be electrically conductive with the first reservoir through the first conductive liquid;
a main supply path;
a main discharge path;
a shared electrode part provided in the main supply path or the main discharge path;
an electrical insulating substrate having a plurality of through holes that do not pass cells but are configured to pass liquid from the first reservoir provided at a first surface of the electrical insulating substrate to a second surface opposite the first surface, wherein each through hole is associated with: a second reservoir provided at the second surface of the electrical insulating substrate to be able to communicate with a respective through hole of the plurality of through holes and hold a second conductive liquid;
a branch supply path connected to the second reservoir and configured to supply the second conductive liquid to the second reservoir from the main supply path;
a branch discharge path connected to the second reservoir and configured to discharge the second conductive liquid from the second reservoir to the main discharge path; and
at least one nonconductive or insulating valve provided in the branch supply path and/or branch discharge path and disposed between the second reservoir and the shared electrode part, the at least one valve able to allow, when open, or stop, when closed, flow of the second conductive liquid and electrical conduction between the second reservoir and the shared electrode part,
wherein the shared electrode part is arranged to be able to share current with said first electrode part associated with the plurality of through holes.
[claim2]
2. The planar patch clamp device according to claim 1, wherein an electric resistance before and after at least one of the valves is 1 megaohm or more when in a closed configuration.
[claim3]
3. The planar patch clamp device according to claim 1, wherein the planar patch clamp device is a culture-type planar patch clamp device.
[claim4]
4. The planar patch clamp device according to claim 3, wherein an extracellular matrix-forming material is deposited at a rim of at least one through hole on the first surface of the substrate.
[claim5]
5. A planar patch clamp system, comprising: the planar patch clamp device according to claim 1;
a control part controlling opening and closing of at least one valve of said planar patch clamp device; and
an electrical detecting part configured for detecting an electrical signal at the first electrode part or the shared electrode part of said planar patch clamp device.
[claim6]
6. The planar patch clamp system according to claim 5, further comprising an optical detecting part for detecting an optical signal derived from a cell arranged on said planar patch clamp device.
[claim7]
7. The planar patch clamp device according to claim 1, wherein the at least one nonconductive or insulating valve comprises a micropath formed by an elastic material, a movable valve adjoining the micropath, and a drive system able to drive the movable valve.
[claim8]
8. The planar patch clamp device according to claim 1, wherein the first electrode part or the shared electrode part is a salt bridge type electrode comprising an electrode container filled with a KCl saturated solution, in which an Ag/AgCl electrode is placed, wherein a tip of the electrode container is made of inorganic porous material that is in contact with the first or second conductive liquid.
[claim9]
9. The planar patch clamp device according to claim 1, wherein the at least one nonconductive or insulating valve comprises at least one nonconductive or insulating valve in the branch supply path and at least one nonconductive or insulating valve in the branch discharge path.
  • 発明者/出願人(英語)
  • URISU TSUNEO
  • WANG ZHI-HONG
  • UNO HIDETAKA
  • NAGAOKA YASUTAKA
  • KOBAYASHI KEI
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
参考情報 (研究プロジェクト等) CREST Creation of Nanosystems with Novel Functions through Process Integration AREA
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