Planar patch clamp device electrodes for said device and cell ion channel current measurement method
|発明の名称 （英語）||Planar patch clamp device electrodes for said device and cell ion channel current measurement method|
An object of the present invention is directed to reducing a noise current by suppressing variations in an applied membrane potential in the planar patch clamp device, thereby enabling accurate measurement of an ion channel current.
A planar patch clamp device comprising: an electrically insulative substrate having at least one fine through holes; a liquid reservoir and an electrode unit disposed on each of surfaces of the through-hole are configured so that the liquid reservoir retains a conductive liquid and the electrode unit is electrically communicate with the liquid reservoir, wherein the electrode comprises (a) an electrode vessel, wherein at least part of a vessel wall is made of an inorganic porous material, (b) an electrode in which a chloride (NmCl) layer is formed on the surface of a noble metal (Nm), and (c) an electrode solution containing (NmCl) and an alkali metal chloride being dissolved at a saturated concentration, is disclosed.
(From EP2801820 A1)
1. The chimney to make the surface of the both sides to (1) electric dielectric borads, and the cell although at the time of passage height, will be able to pass the liquid the defiant liquid respectively more than 1 forms the minute penetration hole, to 1st ostensible side of (2) minute description penetration holes and 2nd ostensible side maintains about the defiant liquid of the misfortune undercurrent department for and that misfortune undercurrent department electrification possibly, to form the electrode department which is arranged, become misfortune undercurrent additional cell arrangement misfortune undercurrent department of (3) 1st ostensible sides, also (4) minute description 1st ostensible sides and below electrode adding of my 2nd ostensible side (a)-(c) element as for under having the planer patch clamp equipment thing with feature.
When the defiant liquid was introduced to (a) minute description misfortune undercurrent department, to that defiant liquid, the electrode courage where at least part of the courage wall is composed of the weapon porosity material.
Within (b) minute description electrode courage to be accommodated, the precious metal (Nm) to the outer layer department the chloride of that precious metal (NmCl) the electrode which formed a layer.
Within (c) minute description electrode courage to charge, the chloride of the minute description precious metal (NmCl) and the electrode solution where the alkali metal chloride is solved at gunfire consistency.
2. There is in a 1st claim,
Misfortune undercurrent department of minute description 1st ostensible side by the material of storehouse fire permeability to be composed, arranges the cell the 1st misfortune undercurrent department for and electrode addition of 1st ostensible side the 2nd misfortune undercurrent department which is arranged and these week sum undercurrent department and father sum undercurrent department the chimney height as a result of sum is narrow becomes accomplished the planer patch clamp equipment the fact that with feature.
3. The 1st claim or there is in a 2nd claim,
Introduction and discharges the misfortune undercurrent additional defiant liquid of minute description 2nd ostensible side with as a result of sum for and to become the chimney, also with to this sum electrode addition of 2nd ostensible side is arranged the planer patch clamp equipment the fact that with feature.
4. The 1st claim or there is in a 2nd claim,
Multiple forms a misfortune undercurrent department to minute description 1st ostensible side, to these each misfortune undercurrent department the nerve cell as for under arranging the planer patch clamp equipment thing with feature.
5. There is in a 4th claim,
Misfortune undercurrent department of minute description multiple to respectively the valley and the valley and the valley the low of connecting forms the home department, and the valley arranges the nerve cell from here, will be able to obstruct the movement a size and the depth, has the home department formation of the nerve cell network possibly does the planer patch clamp equipment the fact that with feature.
6. The 1st claim or there is in a 2nd claim,
The minute description planer patch clamp equipment (a) below and/or (b) compositions as for under having the planer patch clamp equipment thing with feature.
Is connected to the misfortune undercurrent additional fluidal suction device of (a) 2nd ostensible sides, will be able to load a sound pressure to misfortune undercurrent department of this by the fluidal suction device 2nd ostensible side is becoming.
There is in a penetration hole of (b) borads, the cell external matrix formation material which has a cell fixation power to orifice ambient edge of 1st ostensible side is attaching.
11. The 1st claim or introduces the defiant liquid to misfortune undercurrent department of 2nd ostensible side of the planer patch clamp equipment which is recorded to the 2nd claim with together to misfortune undercurrent department of 1st ostensible side the cell which is a measurement target at the time of dispersion to introduce the Keane defiant liquid from, the chimney to make the misfortune undercurrent department of 1st ostensible side and the misfortune undercurrent department of 2nd ostensible side defiantly, the minute description cell sum to prescribed location of undercurrent issue of my 1st ostensible side from arrangement one situation my 1st ostensible side and to interelectrode of electrode issue of my 2nd ostensible side voltage be about under measurement Ion Channel electric current of the cell which is a target as for under measuring the cell ion Channel electric current measuring method thing with feature.
12. There is in a 11th claim,
To 1st ostensible side of the board of the minute description planer patch clamp equipment forms the misfortune undercurrent department of multiple, and the cell which is a measurement target to be the nerve cell, and the defiant liquid is the cell culture fluid of the minute description nerve cell the cell ion Channel electric current measuring method the fact that with feature.
13. There is in a 12th claim,
To the minute description nerve cell in advance the Ca probe for the Ca imaging is introduced, and the cell ion Channel electric current at the time of occurrence of at least cell action potential or to at the time of the electric wave of the action potential as for under occurring the Ca imaging which is an observation of fluorescence as for under including by a means as for under measuring the cell ion Channel electric current measuring method thing with feature.
14. There is in a 13th claim,
Multiple to majority arranges the nerve cell where the minute description Ca probe is introduced to 1st ostensible side of the minute description planer patch clamp equipment with by the nerve cell network where the synapse of these nerve cell connects together each other to do, in the nerve cell of single unit in these people electric current implantation or voltage authorization be with is in the minute description multiple, to the multiple nerve cell does the measurement by the minute description Ca imaging the cell ion Channel electric current measuring method the fact that with feature.
|参考情報 （研究プロジェクト等）||CREST Creation of Nanosystems with Novel Functions through Process Integration AREA|
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