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Method for screening of therapeutic agent for hyperlipemia

外国特許コード	F170009204
整理番号	A261-11WO
掲載日	2017年9月12日
出願国	欧州特許庁（ＥＰＯ）
出願番号	10833235
公報番号	2505660
公報番号	2505660
出願日	平成22年11月25日(2010.11.25)
公報発行日	平成24年10月3日(2012.10.3)
公報発行日	平成26年5月21日(2014.5.21)
国際出願番号	JP2010070959
国際公開番号	WO2011065389
国際出願日	平成22年11月25日(2010.11.25)
国際公開日	平成23年6月3日(2011.6.3)
優先権データ	特願2009-269593 (2009.11.27) JP 2010WO-JP70959 (2010.11.25) WO
発明の名称（英語）	Method for screening of therapeutic agent for hyperlipemia
発明の概要（英語）	(EP2505660) Disclosed are a highly safe treatment method for hyperlipidemia and a therapeutic agent for hyperlipidemia. Specifically, the invention provides a novel method for screening an agent for treating hyperlipidemia, more specifically, a method for screening a substance that can inhibit the production or function of gangliosides, particularly GM3, or inhibit the activity or expression of GM3 synthase to reduce a blood lipid level. A pharmaceutical composition, which can specifically inhibit the production of gangliosides, particularly GM3, thereby effective for hyperlipidemia treatment, and others are also provided.
特許請求の範囲（英語）	[claim1] 1. A method for screening a substance having a blood lipid level lowering activity, which comprises: (i) contacting GM3 synthase with a substrate thereof in the presence or absence of a test substance, (ii) selecting a test substance that decreases the synthesized amount of GM3, (iii) administering the selected test substance to a non-human animal, and (iv) determining the blood lipid level in the non-human animal. [claim2] 2. A method for screening a substance having a blood lipid level lowering activity, which comprises: (i) contacting a test substance with a cell expressing GM3 synthase, (ii) selecting a test substance that decreases the expression level of GM3 synthase, (iii) administering the selected test substance to a non-human animal, and (iv) determining the blood lipid level in the non-human animal. [claim3] 3. The method according to claim 2, wherein the cell expressing GM3 synthase is selected from the group consisting of adipocytes, hepatocytes, vascular endothelial cells, vascular smooth muscle cells, skeletal muscle cells, epithelial cells, neurocytes, fibroblasts, monocytes and macrophages. [claim4] 4. The method according to claim 2, wherein the cell expressing GM3 synthase is a transformant produced by genetic engineering. [claim5] 5. The screening method according to any one of claims 1 to 4, which further comprises selecting a test substance that reduces a blood cholesterol level and/or a blood triglyceride level. [claim6] 6. The method according to any one of claims 1 to 5, wherein the non-human animal has a higher blood lipid level than normal level. [claim7] 7. The method according to any one of claims 1 to 6, wherein the non-human animal is a mouse. [claim8] 8. A pharmaceutical composition effective for treatment of hyperlipidemia, comprising a substance that specifically prevents a production of a ganglioside. [claim9] 9. The composition according to claim 8, wherein the ganglioside is GM3. [claim10] 10. A pharmaceutical composition effective for treatment of hyperlipidemia, comprising an antibody against GM3, a GM3 synthase inhibitor, or a substance that inhibits GM3 synthase expression.
出願人（英語）	JAPAN SCIENCE AND TECHNOLOGY AGENCY
発明者（英語）	INOKUCHI JINICHI NAGAFUKU MASAKAZU HAYAMIZU HIROTAKA
国際特許分類(IPC)	A61K 39/395 抗体；免疫グロブリン；免疫血清，例．抗リンパ球血清 A61K 45/00 Ａ６１Ｋ３１／００～Ａ６１Ｋ４１／００に属さない活性成分を含有する医薬品製剤 A61P 3/06 高脂血症治療剤 A61P 9/10 虚血，アテローム性動脈硬化症の治療のためのもの，例．抗アンギナ，冠状動脈拡張剤，心筋梗塞，網膜症，脳血流障害，腎臓細動脈硬 A61P 43/00 グループＡ６１Ｐ１／００～Ａ６１Ｐ４１／００に展開されていない特殊な目的の医薬 C12N 5/10 外来遺伝物質の導入によって修飾された細胞，例．ウイルス形質転換細胞 C12N 15/09 組換えＤＮＡ技術 C12Q 1/02 生きた微生物を含むもの C12Q 1/48 転移酵素を含むもの C12Q 1/60 コレステロールを含むもの C12Q 1/61 トリグリセリドを含むもの G01N 33/15 医薬 G01N 33/50 生物学的材料，例．血液，尿，の化学分析；生物学的特異性を有する配位子結合方法を含む試験；免疫学的試験 G01N 33/92 脂質，例．コレステロール，を含むもの
欧州特許分類/主・副	A61K045/06 C12Q001/48 C12Q001/60 C12Q001/61 S01N333/910D9 S01N405/10 S01N500/02 S01N800/04B S01N800/32D
指定国	(EP2505660) Contracting States: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
参考情報（研究プロジェクト等）	CREST Clarification of the Biological Functions of Sugar Chains and the Use of this Knowledge in Applied Technologies AREA