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IRRADIATION DEVICE, LASER MICROSCOPE SYSTEM, IRRADIATION METHOD, AND LASER MICROSCOPE DETECTION METHOD

外国特許コード F170009228
整理番号 IP02P002
掲載日 2017年9月14日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2017JP004801
国際公開番号 WO 2017138619
国際出願日 平成29年2月9日(2017.2.9)
国際公開日 平成29年8月17日(2017.8.17)
優先権データ
  • 特願2016-022607 (2016.2.9) JP
発明の名称 (英語) IRRADIATION DEVICE, LASER MICROSCOPE SYSTEM, IRRADIATION METHOD, AND LASER MICROSCOPE DETECTION METHOD
発明の概要(英語) Provided are an irradiation device, a laser microscope system, an irradiation method, and a laser microscope detection method with which the bandwidth of detection light in the form of multiplexed signals can be increased. According to the present invention, laser light beams are separated and caused to enter a first AOD (24) and a second AOD (34) to generate a plurality of beams of first diffracted light and second diffracted light which have different angles of deflection and different levels of frequency shift. A beam splitter (19) superimposes the first diffracted light beams and the second diffracted light beams on each other to generate multiple interfering light beams which have different beat frequencies. An object lens (52) linearly aligns irradiation spots of the multiple interfering light beams in a main scanning direction and irradiates a sample (T) with the interfering light beams. Oscillation of a scan mirror (47a) causes the irradiation spots to move in a sub-scanning direction orthogonal to the main scanning direction. A light detection unit (13) detects fluorescent light emitted from the sample (T) irradiated with the respective interfering light beams.
従来技術、競合技術の概要(英語) BACKGROUND ART
The microscope system using a laser confocal optical system has been known. This laser microscope system, the laser light collected by the objective lens and is irradiated on the sample, the fluorescent or reflected light emitted from the sample by a condensing lens and the focal length through a pinhole disposed at a position to be received by the optical detector. Then, the irradiation position of the laser light 2 by the movement of the orthogonal directions, and scanning the sample surface.
On the other hand, a multi-excitation frequency (FIRE: Fluorescence Imaging using Radiofrequency-tagged Emission) method using a laser technique referred to as the microscope system has been proposed by Patent Document 1. Of the method of the multi-excitation frequency of the laser microscope system, the first laser beam 1, beam splitter 2 is divided into a first laser beam, the deflection angle of the laser light 1 from the first frequency and the magnitude of the output of the diffracted light differs from the acousto-optic deflector (hereinafter, referred to as AOD(Acousto-Optic Deflector) ) and, and the fluorescence response of the photo-detector response speed into consideration in order to lower the beat frequency to shift the frequency of the laser light 2 and the second acousto-optic frequency shifter (hereinafter, referred to as AOFS(Acousto-Optic Frequency Shifter) ) and, to a plurality of diffraction light from the AOD AOFS 2 are superposed from the first laser light, laser light and the diffracted light 2 and the second (beat frequency) a difference in frequency between the excitation light is intensity-modulated in a beam splitter, the sample is irradiated with the excitation light irradiation section and a second color.
The frequency of the laser microscope system in a multi-excitation method, a plurality of irradiated with the excitation light irradiating section is formed by arranging a line-shaped spot, a plurality of illumination spots are moved in one direction. In this way, a plurality of illumination spots in one direction one movement of the 1, the surface of the sample can be scanned. Corresponding to each portion of the illumination spot from the sample, the fluorescence from the fluorescent material in the sample is released. Then, the fluorescence emitted is, the beat frequency of the interference light is irradiated with the light intensity in accordance with the change, by the movement of the illumination spot in the sub scanning direction, the sub-scanning line in accordance with the distribution of the fluorescent substance becomes a modulation. That is, the fluorescence, irradiated with a carrier frequency of the beat frequency of the interference light signal is intensity modulated in accordance with the distribution of the fluorescent substance to an optical signal. Then, the signal from the plurality of light spots irradiated are superimposed in a multiplexed signal such as a photomultiplier tube and the detection light detected by the light detector, from the frequency spectrum, a fluorescent material in the region of movement of the spots irradiated calculate the distribution of the observed image is obtained.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
  • 発明者(英語)
  • MIKAMI Hideharu
  • GODA Keisuke
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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