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ALGINATE LYASE AND METHOD USING ENZYME FOR PRODUCING UNSATURATED URONIC ACID MONOSACCHARIDE UPDATE 新技術説明会

外国特許コード F170009261
整理番号 (S2016-0651-N0)
掲載日 2017年10月24日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2017JP013871
国際公開番号 WO 2017175694
国際出願日 平成29年4月3日(2017.4.3)
国際公開日 平成29年10月12日(2017.10.12)
優先権データ
  • 特願2016-075364 (2016.4.4) JP
発明の名称 (英語) ALGINATE LYASE AND METHOD USING ENZYME FOR PRODUCING UNSATURATED URONIC ACID MONOSACCHARIDE UPDATE 新技術説明会
発明の概要(英語) [Problem] To provide a novel alginate lyase and a method for producing an unsaturated uronic acid monosaccharide using the enzyme.
[Solution] The present invention is achieved by a polypeptide that has an alginate lyase activity and that has an amino acid sequence represented by SEQ ID NO: 1 or an amino acid sequence sharing at least 90% homology with said sequence. This polypeptide is used in a method for producing 4-deoxy-L-erythro-5-hexoseulose uronic acid (DEH), the method comprising: (a) an enzyme addition step for bringing said polypeptide into contact with an alginic acid containing a uronic portion; and (b) an enzymatic action step for keeping the mixture of the alginic acid and the polypeptide at a temperature at which exo-type alginate lyase activity remains active.
従来技術、競合技術の概要(英語) BACKGROUND ART
The kind of the aquatic plants in the world can be seen from the ratio of the amount of production, 700 million tons in 68.6% occupied by the brown algae, the configuration of the polysaccharide is alginate is most often, in about 30%. Is an edible brown algae such as Laminaria Undaria and is used as a part but, for the most part do not use the resources. Brown algae is, arable land and fresh water, without the need for fertilizer, exists naturally in a gel, terrestrial biomass compared to the enzyme reaction can be conducted easily has attracted attention because of their advantages. In particular country Japan are surrounded by the sea, the marine area leading Japan from deconvolution methods, the use of the brown algae as long as it can also be made of a stable source of feedstock material. In addition, the configuration of the polysaccharides include brown algae and is largest in the alginic acid from, the use of the alginate has become an important issue. C5 Β-D - alginic acid epimer mannuronic acid and guluronic acid 2 α-L - one uronic acid glycosidically bound by a linear polymer (Fig. 1), β-D - mannuronic acid is combined with poly (M) block, poly α-L - guluronic acid bound (g) block, mannuronic acid and α-L - β-D - guluronic acid are alternately bound to poly (M, g) are mixed in the fraction of the block 3.
So far, can be metabolized by alginic acid has been found and several types of microorganisms, these microorganisms having alginate lyase. Alginic acid lyase by β-elimination is polysaccharide lyases(PLs) belonging to the glycoside bond. Current, alginate lyase is PL5, PL6, PL7, PL14, PL15, PL17, PL18 family is divided into. In addition, as a truncated, saturated polymer to decompose the unsaturated oligosaccharides oligosaccharides end type with a, unsaturated monomer or an unsaturated monosaccharide sugar polymer is decomposed into exo type 2 of known type. At present, PL5 and family PL7 endo, exo type PL17 family and PL15 thereof. Exo-type alginate lyase unsaturated monosaccharide is generated in the degradation by non-enzymatic reaction by the unsaturated double bonds are cleaved by ring-opening, becomes 4-deoxy-L-erythro-5-hexoseulose uronic acid(DEH). In some marine microorganisms and metabolism DEH fermenting microorganism is modified by reducing the assimilate lactic acid or succinic acid and the like of the organic acids, biofuels such as bioethanol can be made. For this reason, a method of producing DEH efficiently have been studied (Patent Document 1, 2, non-patent document 1-5).
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • MIE UNIVERSITY
  • 発明者(英語)
  • SHIBATA Toshiyuki
  • MIYAKE Hideo
  • MURASE Yoshihiro
  • TANAKA Reiji
  • MORI Tetsushi
  • TAKEYAMA Haruko
  • TAKAHASHI Mami
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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