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MARKER FOR IDENTIFYING GERM CELLS HAVING ENGRAFTABILITY AT HOST GONAD

外国特許コード F170009269
整理番号 (S2016-0498-N0)
掲載日 2017年10月24日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2017JP012112
国際公開番号 WO 2017164391
国際出願日 平成29年3月24日(2017.3.24)
国際公開日 平成29年9月28日(2017.9.28)
優先権データ
  • 特願2016-060914 (2016.3.24) JP
発明の名称 (英語) MARKER FOR IDENTIFYING GERM CELLS HAVING ENGRAFTABILITY AT HOST GONAD
発明の概要(英語) The present invention relates to a marker for identifying germ cells, comprising (I) a marker for identifying germ cells having engraftability at a host gonad and/or (II) a marker for identifying germ cells not having engraftability at a host gonad. The present invention provides a germ cell marker that can identify the presence or absence of engraftability at a host gonad in fish.
従来技術、競合技術の概要(英語) Background of the invention
The artis the present invention, the invention relates to a surrogate host fish techniques, in particular, surrogate host fish in that technique, the germ cells of the host to the implanted separation ability of the engraftment of gonadal germ cells for the presence or absence of the identification marker. The invention also, the germ cells of the present invention using an identification marker, have the ability to engraftment to host the gonads methods for germ cells.
Background artEurope for health-oriented increase in economic development by such as China, the world continues to increase year after year edible seafood consumption, as a result, decrease of the amount of natural marine resources in question. In particular, high demand higher fish and eel and the like as Thunnus orientalis, significantly decrease of the amount of natural resources by overfishing, endangered may change state is continuous with one another. Therefore, marine resources for the purpose of increase in the amount of artificial seedling production and seedling (fry) is the discharge has been studied. In general, seedling production, by specific annihilation Val in order to avoid the onset of disease, genetic variations in individual parent fish is needed, therefore, necessary for growing multiple parent fish. However, depending on the fish species, seedling production is difficult. For example, 100kg or more parent Thunnus orientalis large size fish body weight, swimming method requires the wider of the rearing environment. In addition, the maturation of sperm and egg in sturgeon requires a long period of years. Fish species or human is technically difficult, mating pair 1 1 it is also difficult to fish species, under the control of the multiple parent grown in an artificial fish, sperm and egg bled, cost and technically quite difficult.
Of the fish as seedling production technology, the inventors of the present invention, host (recipient) and dissimilar to the fish in the fish-derived germ cells (donor fish), fish individual host before and after hatching by transplanting into the peritoneal cavity, germ cells can be induced to differentiate into germline found, the separation of the germ cells of the fish in a heterologous host germline has succeeded in differentiation (for example, see Patent Document 1). This technique is, with the anti-surrogate host fish aquaculture techniques or techniques may also be referred to, the parent fish rearing fish sperm and the egg to a problematic, fish were produced by means of the easily a heterologous host, a low-cost simple by crossing technique enables the production of seedling as expected.
In Patent Document 1, primordial germ cells implanted used as germ cells. Specifically, specifically expressed in primordial germ cells with respect to the regulatory region of the gene vasa GFP(Green Fluorescent Protein to: green fluorescent protein) gene has been introduced recombinant gene obtained from the genital ridge of fin-hatched of tissue suspension as a raw material, as an indicator of the green fluorescence by flow cytometry analysis of fluorescence intensity as strong cell population is isolated from the primordial germ cells. However, primordial germ cells, derived from very young embryos before hatch, while the number thereof is approximately 60 per 1 of the fry hatching too low and therefore, is not suitable for commercial mass production.
For the purpose of commercial mass production, a sufficient number of the germ cells of the implanted separation in order to ensure, for example, non-patent document 1 is, in the gonad of donor fish from the cell suspension of the testis, germ cells are separated, the separation germ cells, in that technique surrogate host fish, the abdominal cavity of a method for implanting a host fish individual is disclosed. However, as to separate the germ cell transplantation, separated from testis germ cell transplantation into the host in the case of using the gonads and the percentage of engraftment of an individual, was lower than in the use of primordial germ cells.
Non-patent document 2 is, spermatogonia rainbow trout fry hatching is to be implanted into the abdominal cavity, a portion of the type A spermatogonia (A-SG) were stained only to host the gonads, donors to produce functional gametes from, high engraftment ability of the type A spermatogonia cells, spermatogonial stem cells (SSC) under the belief that the, spermatogonia stem cells focused on Side Population (SP), to the host the gonads high engraftment ability of the concentrating method of the type A spermatogonia is disclosed. However, in this method spermatogonial stem cells is not sufficient and the concentration ratio, the engraftment of the gonads to host germ cells have the ability to obtain an identification marker have not yet been accomplished. Therefore, surrogate host fish in that technique, the germ cells of the host to the implanted separation ability of the gonads in order to determine the presence or absence of engraftment is, before the actual hatching were transplanted onto the host fish individual other than the check, a method for identifying a does not exist.
CITATION LIST
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • NATIONAL UNIVERSITY CORPORATION TOKYO UNIVERSITY OF MARINE SCIENCE AND TECHNOLOGY
  • UNIVERSITY OF TSUKUBA
  • 発明者(英語)
  • HAYASHI Makoto
  • IWASAKI Yoshiko
  • HAYASHI Tetsutaro
  • EBISAWA Masashi
  • SASAGAWA Yohei
  • YOSHIMURA Mika
  • NIKAIDO Itoshi
  • ICHIDA Kensuke
  • YOSHIZAKI Goro
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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