Top > Search of International Patents > METHOD FOR PRODUCING CULTIVATED EPITHELIAL CELL SHEET

METHOD FOR PRODUCING CULTIVATED EPITHELIAL CELL SHEET

Foreign code F170009283
File No. (S2016-0583-N0)
Posted date Nov 7, 2017
Country WIPO
International application number 2017JP015704
International publication number WO 2017183655
Date of international filing Apr 19, 2017
Date of international publication Oct 26, 2017
Priority data
  • P2016-084471 (Apr 20, 2016) JP
Title METHOD FOR PRODUCING CULTIVATED EPITHELIAL CELL SHEET
Abstract A method for producing an epithelial cell sheet, said method comprising a step for cultivating cells, which are derived from oral mucosal epithelial cells, in a serum-free medium on a substrate, wherein the serum-free medium contains: (i) EGF protein or KGF protein; (ii) a B-27 supplement; and (iii) an ROCK inhibitor.
Scope of claims [claim1]
1. Being production method of the epithelium cell seat which includes the process which the cell of buccal cavity mucous membrane epithelium cell origin, bloodless clearly is cultured in the nutrient medium and on the backing material, the aforementioned bloodless is clear the nutrient medium
(i) EGF protein or KGF protein,
(ii) B-27 supplement, and
(iii) ROCK inhibiter
Bloodless it includes clearly it is the nutrient medium, method.
[claim2]
2. The above-mentioned nutrient medium,
(iv) Polysaccharide,
(v) katekin, and
(vi) Corticoid
In the claim 1 which at least furthermore includes 1 where it is selected from the group which consists of kinds method of statement.
[claim3]
3. In claim 1 or 2 the epithelium cell seat which is obtained by the method of statement.
[claim4]
4. (i) EGF protein or KGF protein,
(ii) B-27 supplement, and
(iii) ROCK inhibiter
It includes, bloodless in order the epithelium cell seat to produce the cell of buccal cavity mucous membrane epithelium cell origin as a material it is clear the nutrient medium.
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • KYOTO PREFECTURAL PUBLIC UNIVERSITY
  • Inventor
  • NAKAMURA TAKAHIRO
  • KINOSHITA SHIGERU
  • YOKOO SEIICHI
  • YAMAGAMI SATORU
IPC(International Patent Classification)
Specified countries (WO2017183655)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
Please contact us by E-mail or facsimile if you have any interests on this patent.

PAGE TOP

close
close
close
close
close
close