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MATERIAL FOR CAPTURING AND COLLECTING BLOOD CIRCULATING CELLS BY USING MICROFIBER AND METHOD OF USING SAID MATERIAL

Foreign code F170009286
File No. (S2016-0716-N0)
Posted date Nov 14, 2017
Country WIPO
International application number 2017JP016623
International publication number WO 2017188346
Date of international filing Apr 26, 2017
Date of international publication Nov 2, 2017
Priority data
  • P2016-089023 (Apr 27, 2016) JP
Title MATERIAL FOR CAPTURING AND COLLECTING BLOOD CIRCULATING CELLS BY USING MICROFIBER AND METHOD OF USING SAID MATERIAL
Abstract [Problem] To provide a material for efficiently capturing and collecting specific cells, and a method for capturing and collecting cells by using said material. [Solution] Provided is a material which is used for capturing and collecting cells, and in which a nonwoven fabric made of microfiber is used as a base material, the material being characterized in that the microfiber is modified by a polypeptide having: a peptide site that can be cleaved by a specific enzyme; and a cell adhesion site linked to the peptide site.
Scope of claims [claim1]
1. Being capture and collection of the cell which designates the nonwoven fabrics consisting of the micro fiber as the backing material the material, the aforementioned micro fiber, features that it is decorated by the polypeptide which possesses the cell adhesion characteristic region which is connected to the peptide region and the particular peptide region which can be cut off by the enzyme of specification, the said material.
[claim2]
2. The aforementioned micro fiber, is the polystyrene micro fiber, in claim 1 the material of statement.
[claim3]
3. The aforementioned nonwoven fabrics, have the pore size of the range of 5-20 .micro.m, in claim 1 or 2 the material of statement.
[claim4]
4. The aforementioned nonwoven fabrics, have the fiber diameter of 1-3 .micro.m, either of the claim 1-3 in 1 sections the material of statement.
[claim5]
5. The aforementioned enzyme is koragenaze or the trypsin, the peptide arrangement to which the aforementioned peptide region is recognized in particular koragenaze or the trypsin is included, either of the claim 1-4 in 1 sections the material of statement.
[claim6]
6. It possesses the hydrophilic spacer with the aforementioned peptide region and the aforementioned cell adhesion characteristic region, either of the claim 1-5 in 1 sections the material of statement.
[claim7]
7. The aforementioned cell adhesion characteristic region, is antibody or aputama which possesses cell adhesion characteristic, either of the claim 1-6 in 1 sections the material of statement.
[claim8]
8. The antibody which possesses the aforementioned cell adhesion characteristic, is the maleimide sign antibody, in claim 7 the material of statement.
[claim9]
9. The antibody which possesses the aforementioned cell adhesion characteristic, is the anti- EpCAM antibody, in claim 7 the material of statement.
[claim10]
10. The aforementioned cell, is the blood middle circulating cancer cell, either of the claim 1-9 in 1 sections the material of statement.
[claim11]
11. Either of the claim 1-10 being the method where it captures the cell making use of the material of statement, and it collects in 1 sections,
a) Making the sample contact with the aforementioned material, the process which captures the cell in the aforementioned sample,
b) The process which exfoliates the aforementioned cell which is captured to aforementioned material by processing the aforementioned material with the enzyme, and
c) The description above the process which collects the cell which is exfoliated
It features that it includes, the said method.
[claim12]
12. The aforementioned sample, is the blood, in claim 11 method of statement.
[claim13]
13. The aforementioned process a), with the aforementioned material filtration under reduced pressure the aforementioned sample includes the fact that, in claim 11 or 12 method of statement.
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • THE UNIVERSITY OF TOKYO
  • Inventor
  • TAKAI MADOKA
  • TERAMURA YUJI
  • UEKI TAKAYUKI
  • YOSHIHARA AKIFUMI
  • KONDO YASUHITO
IPC(International Patent Classification)

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