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METHOD FOR PRODUCING PANCREATIC ENDOCRINE CELLS, AND TRANSDIFFERENTIATION AGENT

外国特許コード F170009294
整理番号 S2015-1882-C0
掲載日 2017年11月21日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP082095
国際公開番号 WO 2017073740
国際出願日 平成28年10月28日(2016.10.28)
国際公開日 平成29年5月4日(2017.5.4)
優先権データ
  • 特願2015-212563 (2015.10.29) JP
発明の名称 (英語) METHOD FOR PRODUCING PANCREATIC ENDOCRINE CELLS, AND TRANSDIFFERENTIATION AGENT
発明の概要(英語) A method for producing pancreatic endocrine cells, said method comprising an introduction step in which any of the following genes or gene products are introduced into somatic cells: (A) a mutant GLIS1 gene or gene product thereof having at least 85% sequence identity with the nucleic acid sequence represented by SEQ. ID No. 1 or 2, a Neurogenin-3 gene or gene product thereof, a Pdx1 gene or gene product thereof, and a MafA gene or gene product thereof; (B) a mutant GLIS1 gene or gene product thereof having at least 85% sequence identity with the nucleic acid sequence represented by SEQ. ID No. 1 or 2, a Neurogenin-3 gene or gene product thereof, and a Pdx1 gene or gene product thereof; (C) a GLIS1 gene or gene product thereof, a Neurogenin-3 gene or gene product thereof, a Pdx1 gene or gene product thereof, and a MafA gene or gene product thereof; and (D) a mutant GLIS1 gene or gene product thereof having at least 85% sequence identity with the nucleic acid sequence represented by SEQ. ID No. 1 or 2, a Neurogenin-3 gene or gene product thereof, and a MafA gene or gene product thereof.
従来技術、競合技術の概要(英語) BACKGROUND ART
Of the endocrine pancreas cells, as the material of the regenerative medical treatment of diabetes, in addition, the treatment of diabetes mellitus such as the material used for the screening of drugs that can be used as expected. The method from the viewpoint of regenerative medicine, for example, insulin is insufficient to type Il diabetes I, 1 and one of the endocrine pancreas cells, β cells produce insulin which is expected to be administered. Therefore, the endocrine pancreas cells, in vitro prepared in large quantities is a strong demand for the development.
So far, embryonic stem cells (embryonic stem cell, or less, 'ES cells' referred to), or an induced pluripotent stem cell (induced pluripotent stem cell, or less, ' iPS cells' referred to) using a method of producing β cells have been proposed. However, in the methods, cell culture medium for the various developmental differentiation inhibitor related to and necessary to adjust a culture environment by providing an additional, complicated and, further, reproducibility can be obtained also a problem that is not. In addition, in the methods, can also be produced by cells other than β from, is also a problem in terms of efficiency. Further, to obtain the β cells, required to be at least 21-30- date, can be manufactured in a short time is also a problem that not.
Thus, reproduction is easy and convenient, excellent production efficiency, can be manufactured in a short time and the manufacturing method of the cells of the endocrine pancreas is a strong demand to provide a rapid change in the current situation.
In addition, is GLIS1 (GLIS family zinc finger 1), to improve efficiency in the establishment of improved iPS is known (for example, see Patent Document 1). However, the GLIS1, not through the stem cells, somatic cells of the endocrine pancreas cells directly convert is involved in, is not known at all.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • SAITAMA MEDICAL UNIVERSITY
  • 発明者(英語)
  • MATSUMOTO, Masahito
  • OKAZAKI, Yasushi
  • SUGAHARA, Izumi
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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