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FUSION PROTEIN AND METHOD FOR DETECTING ANTIGEN USING SAME UPDATE

外国特許コード F170009301
整理番号 S2016-0199-C0
掲載日 2017年12月20日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP088061
国際公開番号 WO 2017130610
国際出願日 平成28年12月21日(2016.12.21)
国際公開日 平成29年8月3日(2017.8.3)
優先権データ
  • 特願2016-011475 (2016.1.25) JP
発明の名称 (英語) FUSION PROTEIN AND METHOD FOR DETECTING ANTIGEN USING SAME UPDATE
発明の概要(英語) In order to establish an antigen detection means capable of simply and quickly detecting an antigen and having excellent detection sensitivity, etc., this fusion protein contains an enzyme variant which is activated by multimeric formation, a linker peptide linked to the enzyme variant, and the VH domain or VL domain of an antibody which binds to the linker peptide, and is characterized in that the enzyme variant has an introduced mutation which reduces linking affinity between monomers.
従来技術、競合技術の概要(英語) BACKGROUND ART
Current, is becoming increasingly important in clinical diagnosis and immunoassay technique that has been. It is the adoption of the individual immunoassay, sensitivity, as well as improve specificity, rapid measurement, also simplified has been an important factor. In the current mainstream immunoassay, protein biomarkers in the detection of the sandwich method, competition in the detection of low molecular weight is used as means of measurement. However both of which, after washing several times the main reaction of the label using enzyme-linked immunosorbent assay measuring the enzymatic activity in many cases, the measurement is time-consuming and trouble is several hours. In contrast, the sample and assay reagents mixed with the reaction, a homogeneous immunoassay is detected, it is possible to measure in can be easily and quickly. As homogeneous immunoassay, fluorescent ellipsometry, method EMIT, CEDIA method, OS-FIA method, and the like are known method Quenchbody, competition in the detection of a low molecular weight comparable to that of the conventional practical sensitivity can be obtained in many cases may also be used. However, in the method using fluorescence detection sensitivity of the fluorescent label and the sensitivity is limited to a difficult problem for improving sensitivity while the signal amplification, the sensitivity by signal amplification in the method using enzymes capable of the enzyme stability becomes a problem in many cases. For example, a deletion mutant of β-galactosidase activity between complementary CEDIA method used, OS-ECIA method (non-patent document 1, 2) in, easy-to-substrate has the advantage that the stability of the enzyme but on the other hand, specific activity, the amount of change of a small amount of the active when a severe problem in practical use.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • TOKYO INSTITUTE OF TECHNOLOGY
  • 発明者(英語)
  • UEDA HIROSHI
  • Tung Jinhua
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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