TOP > 外国特許検索 > FUSION PROTEIN AND METHOD FOR DETECTING ANTIGEN USING SAME

FUSION PROTEIN AND METHOD FOR DETECTING ANTIGEN USING SAME

外国特許コード F170009301
整理番号 S2016-0199-C0
掲載日 2017年12月20日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2016JP088061
国際公開番号 WO 2017130610
国際出願日 平成28年12月21日(2016.12.21)
国際公開日 平成29年8月3日(2017.8.3)
優先権データ
  • 特願2016-011475 (2016.1.25) JP
発明の名称 (英語) FUSION PROTEIN AND METHOD FOR DETECTING ANTIGEN USING SAME
発明の概要(英語) In order to establish an antigen detection means capable of simply and quickly detecting an antigen and having excellent detection sensitivity, etc., this fusion protein contains an enzyme variant which is activated by multimeric formation, a linker peptide linked to the enzyme variant, and the VH domain or VL domain of an antibody which binds to the linker peptide, and is characterized in that the enzyme variant has an introduced mutation which reduces linking affinity between monomers.
特許請求の範囲(英語) [claim1]
1. V [H] of the antibody which is connected with linker peptide, and this linker peptide which are connected with the mutant of the enzyme which is activated with the formation of the polymer and the mutant of this enzyme the territory or V [L] being the fusion protein which includes the territory, the mutant of the aforementioned enzyme, the fusion protein which features that it is the mutant where the mutation which decreases the connection affinity between the monomers is introduced.
[claim2]
2. The mutant of the enzyme which is activated with the formation of the polymer, in the claim 1 which features that it is the mutant of I gurukuronidaze the fusion protein of statement.
[claim3]
3. The mutant of I gurukuronidaze, in the claim 2 which features that it is the mutant where the 516th methionine and the 517th thyrosin in amino acid arranging of colon bacillus I gurukuronidaze are substituted to other amino acid the fusion protein of statement.
[claim4]
4. The mutant of I gurukuronidaze, the 516th methionine in amino acid arranging of colon bacillus I gurukuronidaze is substituted by the lysine, in the claim 2 which features that it is the mutant where 517th thyrosin is substituted to glutamic acid the fusion protein of statement.
[claim5]
5. In the fusion protein, the claim either 2 which features that the mutant of 1 or 2 I gurukuronidaze is included or 4 in one section the fusion protein of statement.
[claim6]
6. Claim either 1 or 5 in one section the nuclear acid which features that the cord/code it does the fusion protein of statement.
[claim7]
7. Being the method of detecting the antigen in the sample, the sample, V [H] of the antibody the claim either 1 which includes the territory or 5 in one section V [L] of the fusion protein and the antibody of statement the claim either 1 which includes the territory or 5 method of detection of the antigen which features that it possesses the process which detects process, and the formation of the polymer which are made to contact with the fusion protein of statement one section with the change of enzyme activity.
[claim8]
8. V [H] of the antibody the claim either 1 which includes the territory or 5 in one section V [L] of the fusion protein and the antibody of statement the claim either 1 which includes the territory or 5 the antigen detection kit which features that the fusion protein of statement is included in one section.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • TOKYO INSTITUTE OF TECHNOLOGY
  • 発明者(英語)
  • UEDA HIROSHI
  • DONG JINHUA
国際特許分類(IPC)
指定国 (WO2017130610)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
詳しくお知りになりたい方は下記「問合せ先」まで直接お問合わせください

PAGE TOP

close
close
close
close
close
close