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CELL-EMBEDDED BEADS FOR HAIR REGENERATION AND METHOD FOR PRODUCING SAME, AND KIT FOR HAIR REGENERATION

Foreign code F180009318
File No. (S2016-0752-N0)
Posted date Jan 24, 2018
Country WIPO
International application number 2017JP021770
International publication number WO 2017217393
Date of international filing Jun 13, 2017
Date of international publication Dec 21, 2017
Priority data
  • P2016-120808 (Jun 17, 2016) JP
Title CELL-EMBEDDED BEADS FOR HAIR REGENERATION AND METHOD FOR PRODUCING SAME, AND KIT FOR HAIR REGENERATION
Abstract Provided are cell-embedded beads for hair regeneration having high hair-regenerating activity, and a method for producing the same. The cell-embedded beads for hair regeneration contain mesenchymal cells and biocompatible hydrogel. The method for producing cell-embedded beads for hair regeneration is a method provided with a cell-embedded cured gel formed body preparation step that prepares liquid droplets containing mesenchymal cells and biocompatible hydrogel, cures the biocompatible hydrogel, and prepares a cell-embedded cured gel formed body, and an aggregation step that suspension cultures the cell-embedded cured gel formed body and induces aggregation by the tractive force of the cells.
Outline of related art and contending technology BACKGROUND ART
To establish enough for clinical application of regenerative medicine is hair follicles, hair follicles reproduction has a structure in which the normal tissue, hair implantation site is suitable for the bristles, is formed, needs to be extended. Ectoderm including skin appendages such as the hair of the appendages is, typically, in the embryonic, epithelial cells and mesenchymal cells and which is generated by the interaction. Ectodermal one of the appendages of the hair follicles, growth and regression of an individual over the lifetime (hair cycle) are repeated, the playback portion in the growth of hair, hair follicle organ development phase can be induced by molecular mechanism as is known. In addition, the hair in the reproduction period of the hair, mesenchymal cells induced by the papillary hair cells are considered. That is, in the growth phase, the hair follicles mesenchymal epithelial stem cells differentiated papillary hair cells by the cells induced by the hair portion is reproduced.
Toward the hair follicles to which the mesenchymal cell reproduction (papillary dermis and hair root sheath cell of the cell) by replacing the hair follicle and hair follicles reproduction of the variable region have the ability to induce angiogenesis hair follicles by mesenchymal cells, epithelial cells and mesenchymal cells and the reconstruction of hair follicles and the like have been attempted. Specifically, a plurality of kinds of cells derived from somatic Wnt signal active agent was added a mixed culture in culture can be used, such as a primitive method of forming a hair follicle organ (for example, see Patent Document 1.), Mesenchymal cells of the cell clump hair follicles (spheroid) epithelial cells outside of the sphere is adhered to a method of producing an artificial hair (for example, see Patent Document 2.), And a hair disc cells and epithelial cells and is implanted with the spheroids, a reproduction method of the hair (for example, see Non-Patent Document 1.) And the like.
Scope of claims (In Japanese)[請求項1]
間葉系細胞と、生体適合性ハイドロゲルとを含有することを特徴とする毛髪再生用細胞包埋ビーズ。
[請求項2]
前記生体適合性ハイドロゲルがゲル化する細胞外マトリックス成分である請求項1に記載の毛髪再生用細胞包埋ビーズ。
[請求項3]
前記細胞外マトリックス成分がI型コラーゲンである請求項2に記載の毛髪再生用細胞包埋ビーズ。
[請求項4]
前記間葉系細胞の細胞密度が2×105cells/cm3以下である請求項1~3のいずれか一項に記載の毛髪再生用細胞包埋ビーズ。
[請求項5]
間葉系細胞と、生体適合性ハイドロゲルとを含有する液滴を作製し、前記生体適合性ハイドロゲルを硬化させ、細胞包埋硬化ゲル形成体を作製する細胞包埋硬化ゲル形成体作製工程と、
前記細胞包埋硬化ゲル形成体を浮遊培養し、細胞の牽引力により凝集させる凝集工程と、
を備えることを特徴とする毛髪再生用細胞包埋ビーズの製造方法。
[請求項6]
前記細胞包埋硬化ゲル形成体作製工程において、撥水性表面を有する支持体に滴下し、前記液滴を作製する請求項5に記載の毛髪再生用細胞包埋ビーズの製造方法。
[請求項7]
前記細胞包埋硬化ゲル形成体作製工程において、前記液滴に含まれる前記間葉系細胞の濃度が、5×105cells/mL以上1×108cells/mLである請求項5又は6に記載の毛髪再生用細胞包埋ビーズの製造方法。
[請求項8]
請求項1~4のいずれか一項に記載の毛髪再生用細胞包埋ビーズと、
上皮系細胞と、
を備えることを特徴とする毛髪再生用キット。
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • YOKOHAMA NATIONAL UNIVERSITY
  • Inventor
  • FUKUDA Junji
  • KAGEYAMA Tatsuto
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
(In Japanese)掲載特許について詳しくお知りになりたい方はHPの「お問い合わせ」ページにてお問い合わせください。

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