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METHOD FOR PRODUCING VACCINIA VIRUS EXPRESSING FOREIGN GENE

外国特許コード F180009355
整理番号 (S2016-0912-N0)
掲載日 2018年4月19日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2017JP025486
国際公開番号 WO 2018012570
国際出願日 平成29年7月13日(2017.7.13)
国際公開日 平成30年1月18日(2018.1.18)
優先権データ
  • 特願2016-138712 (2016.7.13) JP
発明の名称 (英語) METHOD FOR PRODUCING VACCINIA VIRUS EXPRESSING FOREIGN GENE
発明の概要(英語) The purpose of the present invention is to provide a vaccinia virus which expresses a foreign gene and is capable of killing cancer cells, and a medicine for cancer treatment which comprises the vaccinia virus. The present invention relates to a vaccinia virus to which a suicide gene is introduced as a foreign gene, said suicide gene being selected from the group consisting of cytosine deaminase (CD) gene, uracil phosphoribosyltransferase (UPRT) gene and herpes simplex thymidine kinase (HS-tk) gene.
特許請求の範囲(英語) [claim1]
1. Exogenous gene as a gene (CD) cytosine, and uracil phosphoribosyltransferase (UPRT) gene of the herpes simplex thymidine kinase (HS-tk) gene is selected from the group consisting of the suicide gene has been introduced is vaccinia virus.
[claim2]
2. Of the suicide gene as foreign genes in gene and uracil phosphoribosyltransferase (CD) TK (UPRT) according to claim 2 introduced gene vaccinia virus.
[claim3]
3. A vaccinia virus, LC16 lines or LC16mO strain JFHl, according to claim 1 or 2 vaccinia virus.
[claim4]
4. Is of the suicide gene, vaccinia virus growth factor (VGF) and/or inserted into the O1L gene, O1L and/or vaccinia virus growth factor (VGF) function of the gene are missing, in a normal cell does not grow, specific proliferation of cancer cells, cancer cells with a specific injury oncolytic has, further action of the suicide gene prliferation vaccinia virus cancer cells.
[claim5]
5. Of the suicide gene is a gene and uracil phosphoribosyltransferase (CD) TK (UPRT) gene, O1L gene vaccinia virus growth factor (VGF) and inserted into one of, a marker gene is vaccinia virus growth factor (VGF) and inserted into the other of the O1L gene, O1L gene vaccinia growth factor (VGF) and are missing and function, in a normal cell does not grow, specifically in the cancer cells Schizochyhflium, cancer cells with a specific fault has oncolytic to, under the action of addition of the suicide gene prliferation cancer cells, according to claim 4 vaccinia virus.
[claim6]
6. Any one of claims 1-5 vaccinia virus to a pharmaceutical composition for the treatment of cancer.
[claim7]
7. For the treatment of cancer of the pharmaceutical composition according to claim 6 5 - fluorocytosine and neofurtulon (5-FC) or 5 - (5-FU) and a combination of the kit.
[claim8]
8. 5- Fluorocytosine neofurtulon (5-FC) or 5 - (5-FU) in combination with, a pharmaceutical composition according to claim 6.
[claim9]
9. A vaccinia virus Fryburg gene or untranslated region of a marker gene has been inserted in a method of fabricating a vaccinia virus, intended to be inserted into a selectable marker gene to endogenous vaccinia DNA encoding non-translated region or to the marker genes in the DNA structure is inserted into the insertion of the marker gene is introduced into a plasmid for vaccinia virus, by means of homologous recombination vaccinia virus endogenous or untranslated region comprises inserting a marker gene.
[claim10]
10. Intended to be inserted into the selectable marker gene of a gene encoding Fryburg vaccinia virus DNA or non-translated region DNA marker gene inserted into the insertion of the marker gene structure is introduced into a plasmid for vaccinia virus, by means of homologous recombination vaccinia virus endogenous or untranslated region by inserting a marker gene, endogenous vaccinia virus or untranslated regions of the marker gene is inserted into, further, endogenous or untranslated region is inserted into the other of the marker gene comprises replacing the foreign genes, gene markers generated by the loss of signal indication of the marker gene is replaced with foreign genes are introduced into the determined vaccinia virus, a method of introducing exogenous genes vaccinia virus.
[claim11]
11. A non-translation or endogenous Kx_Hi marker gene region of vaccinia virus is inserted in the inserted marker gene comprises replacing other foreign genes, gene markers generated by the loss of signal indication of the marker gene into which the foreign gene replaced is determined to be introduced into a vaccinia virus, a method of introducing exogenous genes vaccinia virus.
[claim12]
12. A vaccinia virus, LC16 lines or LC16mO strain, method according to any one of claims 9-11.
[claim13]
13. That a marker gene, luciferase (LUC) gene, fluorescent protein genes, β GtJS (GUS) gene, chloramphenicol acetyl transferase (CAT) gene and β - galactosidase (LacZ) gene is selected from the group consisting of any one of claims 9-12.
[claim14]
14. Of a marker gene in the gene Fryburg inserted, hemagluttinin (HA) gene, thymidine kinase (TK) gene, O1L gene and vaccinia growth factor (VGF) gene is selected from the group consisting of any one of claims 9-13.
[claim15]
15. Into which the foreign gene introducing immunopotentiators or cytotoxic effects a therapeutic effect, or cancer, viral, bacterial or protazoal of DNA encoding the antigen, or a suicide gene is, in the method of any one of claims 10-14.
[claim16]
16. TK foreign gene (CD) gene, the gene and the herpes uracil phosphoribosyltransferase (UPRT) (HS-tk) gene is selected from the group consisting of a method according to claim 15 of.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • TOTTORI UNIVERSITY
  • 発明者(英語)
  • NAKAMURA TAKAFUMI
  • NAKATAKE MOTOMU
  • KUROSAKI HAJIME
  • HORITA KOUSUKE
国際特許分類(IPC)
指定国 (WO201812570)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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