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METHOD FOR PRODUCING VACCINIA VIRUS EXPRESSING FOREIGN GENE

Foreign code F180009355
File No. (S2016-0912-N0)
Posted date Apr 19, 2018
Country WIPO
International application number 2017JP025486
International publication number WO 2018012570
Date of international filing Jul 13, 2017
Date of international publication Jan 18, 2018
Priority data
  • P2016-138712 (Jul 13, 2016) JP
Title METHOD FOR PRODUCING VACCINIA VIRUS EXPRESSING FOREIGN GENE
Abstract The purpose of the present invention is to provide a vaccinia virus which expresses a foreign gene and is capable of killing cancer cells, and a medicine for cancer treatment which comprises the vaccinia virus. The present invention relates to a vaccinia virus to which a suicide gene is introduced as a foreign gene, said suicide gene being selected from the group consisting of cytosine deaminase (CD) gene, uracil phosphoribosyltransferase (UPRT) gene and herpes simplex thymidine kinase (HS-tk) gene.
Scope of claims [claim1]
1. Exogenous gene as a gene (CD) cytosine, and uracil phosphoribosyltransferase (UPRT) gene of the herpes simplex thymidine kinase (HS-tk) gene is selected from the group consisting of the suicide gene has been introduced is vaccinia virus.
[claim2]
2. Of the suicide gene as foreign genes in gene and uracil phosphoribosyltransferase (CD) TK (UPRT) according to claim 2 introduced gene vaccinia virus.
[claim3]
3. A vaccinia virus, LC16 lines or LC16mO strain JFHl, according to claim 1 or 2 vaccinia virus.
[claim4]
4. Is of the suicide gene, vaccinia virus growth factor (VGF) and/or inserted into the O1L gene, O1L and/or vaccinia virus growth factor (VGF) function of the gene are missing, in a normal cell does not grow, specific proliferation of cancer cells, cancer cells with a specific injury oncolytic has, further action of the suicide gene prliferation vaccinia virus cancer cells.
[claim5]
5. Of the suicide gene is a gene and uracil phosphoribosyltransferase (CD) TK (UPRT) gene, O1L gene vaccinia virus growth factor (VGF) and inserted into one of, a marker gene is vaccinia virus growth factor (VGF) and inserted into the other of the O1L gene, O1L gene vaccinia growth factor (VGF) and are missing and function, in a normal cell does not grow, specifically in the cancer cells Schizochyhflium, cancer cells with a specific fault has oncolytic to, under the action of addition of the suicide gene prliferation cancer cells, according to claim 4 vaccinia virus.
[claim6]
6. Any one of claims 1-5 vaccinia virus to a pharmaceutical composition for the treatment of cancer.
[claim7]
7. For the treatment of cancer of the pharmaceutical composition according to claim 6 5 - fluorocytosine and neofurtulon (5-FC) or 5 - (5-FU) and a combination of the kit.
[claim8]
8. 5- Fluorocytosine neofurtulon (5-FC) or 5 - (5-FU) in combination with, a pharmaceutical composition according to claim 6.
[claim9]
9. A vaccinia virus Fryburg gene or untranslated region of a marker gene has been inserted in a method of fabricating a vaccinia virus, intended to be inserted into a selectable marker gene to endogenous vaccinia DNA encoding non-translated region or to the marker genes in the DNA structure is inserted into the insertion of the marker gene is introduced into a plasmid for vaccinia virus, by means of homologous recombination vaccinia virus endogenous or untranslated region comprises inserting a marker gene.
[claim10]
10. Intended to be inserted into the selectable marker gene of a gene encoding Fryburg vaccinia virus DNA or non-translated region DNA marker gene inserted into the insertion of the marker gene structure is introduced into a plasmid for vaccinia virus, by means of homologous recombination vaccinia virus endogenous or untranslated region by inserting a marker gene, endogenous vaccinia virus or untranslated regions of the marker gene is inserted into, further, endogenous or untranslated region is inserted into the other of the marker gene comprises replacing the foreign genes, gene markers generated by the loss of signal indication of the marker gene is replaced with foreign genes are introduced into the determined vaccinia virus, a method of introducing exogenous genes vaccinia virus.
[claim11]
11. A non-translation or endogenous Kx_Hi marker gene region of vaccinia virus is inserted in the inserted marker gene comprises replacing other foreign genes, gene markers generated by the loss of signal indication of the marker gene into which the foreign gene replaced is determined to be introduced into a vaccinia virus, a method of introducing exogenous genes vaccinia virus.
[claim12]
12. A vaccinia virus, LC16 lines or LC16mO strain, method according to any one of claims 9-11.
[claim13]
13. That a marker gene, luciferase (LUC) gene, fluorescent protein genes, β GtJS (GUS) gene, chloramphenicol acetyl transferase (CAT) gene and β - galactosidase (LacZ) gene is selected from the group consisting of any one of claims 9-12.
[claim14]
14. Of a marker gene in the gene Fryburg inserted, hemagluttinin (HA) gene, thymidine kinase (TK) gene, O1L gene and vaccinia growth factor (VGF) gene is selected from the group consisting of any one of claims 9-13.
[claim15]
15. Into which the foreign gene introducing immunopotentiators or cytotoxic effects a therapeutic effect, or cancer, viral, bacterial or protazoal of DNA encoding the antigen, or a suicide gene is, in the method of any one of claims 10-14.
[claim16]
16. TK foreign gene (CD) gene, the gene and the herpes uracil phosphoribosyltransferase (UPRT) (HS-tk) gene is selected from the group consisting of a method according to claim 15 of.
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • TOTTORI UNIVERSITY
  • Inventor
  • NAKAMURA TAKAFUMI
  • NAKATAKE MOTOMU
  • KUROSAKI HAJIME
  • HORITA KOUSUKE
IPC(International Patent Classification)
Specified countries (WO201812570)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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