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METHOD FOR DISCRIMINATING EPINEPHELUS FUSCOGUTTATUS HAVING GENETIC TRAIT OF HIGH GROWTH

Foreign code F180009372
File No. (S2016-0497-N0)
Posted date Apr 19, 2018
Country WIPO
International application number 2017JP023930
International publication number WO 2018008511
Date of international filing Jun 29, 2017
Date of international publication Jan 11, 2018
Priority data
  • P2016-132224 (Jul 4, 2016) JP
Title METHOD FOR DISCRIMINATING EPINEPHELUS FUSCOGUTTATUS HAVING GENETIC TRAIT OF HIGH GROWTH
Abstract The present invention develops a marker of a genetic trait of high growth in Epinephelus fuscoguttatus to thereby provide a method whereby a fish having a genetic trait of high growth can be discriminated without killing any individuals. By using genetic markers developed with regard to Epinephelus bruneus , a genetic map of E. fuscoguttatus was prepared. Cultured E. fuscoguttatus fish were classified into a heavy-weight group and a light-weight group and then subjected to QTL analysis with use of the aforesaid genetic markers. As a result, six main genetic markers affecting the genetic trait of high growth were found out, said genetic markers being Ebr00010FRA (SEQ ID NO: 1) and Ebr00935FRA (SEQ ID NO: 2) which are DNA marker loci on linkage group 12 and Ebr00846FRA (SEQ ID NO: 3), Ebr00924FRA (SEQ ID NO: 4), CfuSTR210 (SEQ ID NO: 5) and Ebr01255FRA (SEQ ID NO: 6) which are DNA marker loci on linkage group 21.
Scope of claims [claim1]
1. - (6) Any one of the DNA marker locus sequence following (1) the signal sequence or a part thereof, and the micro satellite array that consists of a polynucleotide having nucleotide sequence, the number of growth having a genetic trait akamadarahata genetic markers for identifying. (1) 12 DNA markers on linkage group (sequence number 1) of the seat Ebr00010FRA (the 487-534 position corresponding to a sequence of a microsatellite. 12) (2) DNA markers on linkage group (sequence number 2) of the seat Ebr00935FRA (the 131-162 position corresponding to a sequence of a microsatellite. 21) (3) DNA markers on linkage group (sequence number 3) of the seat Ebr00846FRA (the 173-206 position corresponding to a sequence of a microsatellite. 21) (4) DNA markers on linkage group (sequence number 4) of the seat Ebr00924FRA (the 321-344 position corresponding to a sequence of a microsatellite. 21 DNA markers on the seat CfuSTR 210 of the linkage group) (5) (SEQ ID NO:5) (the 106-127 position corresponding to a sequence of a microsatellite. 21) (6) DNA markers on linkage group (sequence number 6) of the seat Ebr01255FRA (the 113-136 position corresponding to a sequence of a microsatellite.)
[claim2]
2. Akamadarahata, the egg or their DNA extracted from for the workpiece, according to claim 1 consisting of genetic markers detected, a method for identifying genetic traits of the growing akamadarahata.
[claim3]
3. Comprising the step of the number of growth having a genetic trait following akamadarahata identification method. 1) Akamadarahata, the egg or extracted from the work piece thereof for DNA, amplifying the polynucleotide according to claim 1 genetic marker, 2) as a result of subcultured separately maintained, the number of growth having a genetic trait diphyletic akamadarahata for recognized, the 1) step of performing the same steps, and 3) 1) and 2) comparing the amplified result in the step of, if they match in, akamadarahata growth having a genetic trait and the step of identifying
[claim4]
4. Step 3) in which, when compared with a size of a polynucleotide, and having a genetic trait akamadarahata is the number of growth method according to claim 3 identifying.
[claim5]
5. Any one of the following (1) - (6) DNA marker locus from among the sequence of at least 18 oligonucleotide consisting of two bases, and the micro satellite array thereof so as to sandwich the one of the base sequence of the 2, the base sequence of the polynucleotide comprised on the one hand, and the other consisting of the nucleotide sequence of oligonucleotides complementary to the oligonucleotide, or a complementary sequence to the one PCR primer oligonucleotides of 2. (1) 12 DNA markers on linkage group (sequence number 1) of the seat Ebr00010FRA (the 487-534 position corresponding to a sequence of a microsatellite. 12) (2) DNA markers on linkage group (sequence number 2) of the seat Ebr00935FRA (the 131-162 position corresponding to a sequence of a microsatellite. 21) (3) DNA markers on linkage group (sequence number 3) of the seat Ebr00846FRA (the 173-206 position corresponding to a sequence of a microsatellite. 21) (4) DNA markers on linkage group (sequence number 4) of the seat Ebr00924FRA (the 321-344 position corresponding to a sequence of a microsatellite. 21 DNA markers on the seat CfuSTR 210 of the linkage group) (5) (SEQ ID NO:5) (the 106-127 position corresponding to a sequence of a microsatellite. 21) (6) DNA markers on linkage group (sequence number 6) of the seat Ebr01255FRA (the 113-136 position corresponding to a sequence of a microsatellite.)
[claim6]
6. Is the number of growth having a genetic trait akamadarahata for identifying whether or not the diagnostic kit, the kit according to claim 5 PCR primers for.
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • TOKYO UNIVERSITY OF MARINE SCIENCE AND TECHNOLOGY
  • DEPARTMENT OF FISHERIES MINISTRY OF AGRICULTURE AND COOPERATIVES
  • Inventor
  • SAKAMOTO TAKASHI
  • OZAKI AKIYUKI
  • KANONKPORN KESSUWAN
IPC(International Patent Classification)
Specified countries (WO201808511)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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