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METHOD FOR DETECTING PROTEIN PRESENT IN MEMBRANE OF EXOSOME

外国特許コード F180009380
整理番号 (S2016-1032-N0)
掲載日 2018年4月19日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2017JP029064
国際公開番号 WO 2018030511
国際出願日 平成29年8月10日(2017.8.10)
国際公開日 平成30年2月15日(2018.2.15)
優先権データ
  • 特願2016-158985 (2016.8.12) JP
発明の名称 (英語) METHOD FOR DETECTING PROTEIN PRESENT IN MEMBRANE OF EXOSOME
発明の概要(英語) The present invention provides a method for detecting proteins present in membranes of exosomes in a biological sample, wherein the method includes a step for bringing the exosomes isolated from said biological sample into contact with antibodies for said proteins, in a dispersed state in a bovine-serum albumin solution, thus detecting complexes formed of said proteins and said antibodies.
従来技術、競合技術の概要(英語) BACKGROUND ART
Exosomes is, a diameter of about 40nm - to about 150nm secreted from cells and vesicles, proteins, mRNA, miRNA molecules such as impregnated therein. Tumor cells by tumor exosomes secreted from the cell type for containing the different molecules, the molecules of the derived exosomes has attracted attention as a biomarker. In recent years, exosomes derived from cancer cells in serum tumor marker is used as the diagnosis of cancer have been reported (Non-Patent Document 1 and 2). However, present on exosomes and the measurement of the protein, CD9, CD63, CD81 are not limited to a molecule such as, cell function and is related to a family of membrane proteins such as integrin CEA, even though the number of solutions exist, their protein exosomes on the film is not a report on the measurement.
Current, is in the blood of carcinoembryonic antigen (carcinoembryonic antigen,CEA,CEACAM 5), and general clinical tumor markers, such as colon cancer recurrence are used to diagnose. A molecular weight of about 20 million of the CEA and glycoprotein, fetal tissue and human colon cancer is found in the intestinal tract, also, a plurality of proteins having a structure similar to that of the CEA was also found. These are, CEA family, a new unified classification name is determined, thereby one of the 2 family of CEA, CEACAM(CEA-related cell adhesion molecule) PSG(pregnancy-specific glycoprotein) classified and resulted in. CEA CEACAM CEACAM 1,CEACAM 3,CEACAM 4,CEACAM 6,CEACAM 7 and CEACAM 8 with belonging to the group is. However, the recurrence of cancer CEA is used as a diagnostic marker, markers for early diagnosis of cancer and is not used as a report. Then, other CEA CEA family member is used as a tumor marker is not reported.
Also, in general, is used as a tumor marker proteins such as CEA in the diagnosis of cancer, false positive or false-negative rate is found in, for a false positive does not need to be checked or, for a false negative or delayed the discovery of cancer recurrence, unnecessary to create a burden on a patient. Therefore, a method of detecting the protein with high accuracy is demanded.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • WAKAYAMA MEDICAL UNIVERSITY
  • 発明者(英語)
  • YOKOYAMA, Shozo
  • YAMAUE, Hiroki
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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