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METHOD FOR DETECTING PROTEIN PRESENT IN MEMBRANE OF EXOSOME

外国特許コード F180009380
整理番号 (S2016-1032-N0)
掲載日 2018年4月19日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2017JP029064
国際公開番号 WO 2018030511
国際出願日 平成29年8月10日(2017.8.10)
国際公開日 平成30年2月15日(2018.2.15)
優先権データ
  • 特願2016-158985 (2016.8.12) JP
発明の名称 (英語) METHOD FOR DETECTING PROTEIN PRESENT IN MEMBRANE OF EXOSOME
発明の概要(英語) The present invention provides a method for detecting proteins present in membranes of exosomes in a biological sample, wherein the method includes a step for bringing the exosomes isolated from said biological sample into contact with antibodies for said proteins, in a dispersed state in a bovine-serum albumin solution, thus detecting complexes formed of said proteins and said antibodies.
特許請求の範囲(英語) [claim1]
1. In a biological sample exists in the film of exosomes method of detection of the protein, and dispersed in a solution of bovine serum albumin, exosomes and isolated from said biological sample, the antibody against the protein is contacted with and, of the antibody with a protein comprising the step of detecting the complex, method.
[claim2]
2. Wherein the concentration of the solution is 0.5% bovine serum albumin (w/v) -5% (w/v) in the, method according to claim 1.
[claim3]
3. Wherein the solution concentration is bovine serum albumin (w/v) is 1%, method according to claim 2.
[claim4]
4. Wherein the bovine serum albumin and dispersed in the solution, the concentration of exosomes isolated from a biological sample, in the biological samples 1.5 times -10 times the concentration of exosomes, method according to any one of claims 1-3.
[claim5]
5. Wherein the bovine serum albumin and dispersed in the solution, the concentration of exosomes isolated from a biological sample, in the biological samples -5 times a concentration of 2.5 times the exosomes, method according to claim 4.
[claim6]
6. A frozen biological sample when the sample, the sample 4 Kpse-60 Kpse further comprising the step of melting, method according to any one of claims 1-5.
[claim7]
7. The melting step is carried out in 4 Kpse-37 °, method according to claim 6.
[claim8]
8. Exosomes exists in the film of the protein is a glycoprotein, a method of any one of claims 1-7.
[claim9]
9. Is a member of the family CEA glycoprotein, method according to claim 8.
[claim10]
10. CEA family member, CEA, CEACAM 1, and CEACAM 6 is selected from the group consisting of 1 or more from the, method according to claim 9.
[claim11]
11. In which the living sample is body fluid, the method of any one of claims 1-10.
[claim12]
12. The biological fluid is serum or plasma, method according to claim 11.
[claim13]
13. For detecting cancer distant metastases, the method of any one of claims 1-12.
[claim14]
14. The cancer is colon cancer, gastric cancer, esophageal cancer is selected from the group consisting of 1 and greater than or equal, method according to claim 13.
[claim15]
15. The cancer is colon cancer, method according to claim 14.
[claim16]
16. Inspection for the diagnosis of cancer distant metastases method by the method according to claim 1, (1), exosomes in a biological sample derived from a subject exists in the film of the proteins were detected, the measured concentration of the protein, (2) (1) the concentration of protein obtained by includes comparing with the reference value, the concentration of the protein in the subject is higher than the reference value is indicative of distant metastasis of cancer, method.
[claim17]
17. In which the reference value, the remote presence or absence of metastasis of known in the patient population based on ROC curve is set in the cut-off point, method according to claim 16.
[claim18]
18. The cancer is colon cancer, gastric cancer, esophageal cancer is selected from the group consisting of 1 and greater than or equal, method according to claim 16 or 17.
[claim19]
19. In which the cancer is colon cancer, method according to claim 18.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • WAKAYAMA MEDICAL UNIVERSITY
  • 発明者(英語)
  • YOKOYAMA SHOZO
  • YAMAUE HIROKI
国際特許分類(IPC)
指定国 (WO201830511)
National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG

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