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CELL PREPARATION AND METHOD FOR PRODUCING CELL PREPARATION

Foreign code F180009388
File No. (S2016-0900-N0)
Posted date Apr 20, 2018
Country WIPO
International application number 2017JP031246
International publication number WO 2018043596
Date of international filing Aug 30, 2017
Date of international publication Mar 8, 2018
Priority data
  • P2016-168543 (Aug 30, 2016) JP
Title CELL PREPARATION AND METHOD FOR PRODUCING CELL PREPARATION
Abstract The present invention provides: a method for producing a cell culture for the promotion of angiogenesis or the promotion of axonal outgrowth, particularly the treatment of a cerebrovascular disorder, an ischemic heart disease or a traumatic encephalomyeloneuropathy, said method comprising culturing cells including microglial cells and/or monocytes under low oxygen concentration and/or low sugar concentration conditions to produce the culture; a cell preparation produced by the method; and a method for treating a cerebrovascular disorder, an ischemic heart disease or a traumatic encephalomyeloneuropathy using the cell preparation.
Outline of related art and contending technology BACKGROUND ART
Brain infarction, ischemic necrosis of the brain regional brain function caused by the failure point, the necessary care and disease, cancer and heart disease and 3 are arranged in one of the major causes. Cerebral infarcts, from the viewpoint of the mechanism of action, thrombotic, embolic, hemodynamic property is classified into, from side surfaces of the clinical findings aiding in the cerebral infarction, cardiogenic cerebral embolism, lacunar infarction such as classifications.
Ischemia, cerebral vascular lesions such as arteriosclerosis, thrombus or cardiogenic local cerebral blood flow is blocked by us, in a central portion of the ischemic neuronal cell death is caused due to energy depletion. After ischemia, poor nerve regeneration, chronic symptoms is difficult for the current recovery, there is a possibility that some sequelae half. Ischemia the center of the side edge part of the nerve regeneration as processes, and triggers that revascularization is assumed. As the target which has been desired the development of therapeutics.
For the treatment of cerebral infarction have been various attempts have been made, is not sufficient, in recent years methods of treatment using a living cell has been attempted (Patent Document 1 and 2, see Non Patent Literature 1).
Patent Document 1 is, taken from the blood or bone marrow mesenchymal stem cells to the cells, without using an anticoagulant such as heparin was taken, using the method for treating a disease such as cerebral infarction has been described. Patent Document 2 is, for the treatment of cerebral infarction using MUSE cells and a method.
In addition, bone marrow-derived monocytes mouse cerebral infarction model (CD115 - positive cells) can be administered intravenously, infarcted and proceeds to present cells, the effect is confirmed, the effect is not sufficient (see Non-Patent Document 1).
Scope of claims (In Japanese)[請求項1]
血管新生の促進または軸索伸展の促進のための細胞培養物の製造方法であって、ミクログリアおよび/または単球を含む細胞群を、低酸素濃度および/または低糖濃度の条件下で培養することにより前記培養物を生成することを含む、方法。
[請求項2]
前記培養物が脳血管障害、虚血性心疾患または外傷性脳脊髄神経障害の治療用である、請求項1に記載の方法。
[請求項3]
前記単球を含む細胞群は、末梢血細胞又はその細胞分画である、請求項1または2に記載の細胞製剤の製造方法。
[請求項4]
前記単球を含む細胞群は、末梢血から採取した単核球を含む画分である、請求項1~3の何れか1項に記載の方法。
[請求項5]
低酸素濃度は、1%未満の酸素濃度である、請求項1~4の何れか1項に記載の方法。
[請求項6]
低糖濃度は、1.0g/L以下の糖濃度である、請求項1~5の何れか1項に記載の方法。
[請求項7]
前記細胞群を低酸素濃度かつ低糖濃度の条件下で24時間未満培養する、請求項1~6の何れか1項に記載の方法。
[請求項8]
前記培養物を洗浄した後、容器に封入することをさらに含む、請求項1~7の何れか1項に記載の方法。
[請求項9]
脳血管障害、虚血性心疾患または外傷性脳脊髄神経障害の治療に十分な血管新生の促進および/または軸索伸展の促進能力を有するミクログリアおよび/または単球を含む、脳血管障害、虚血性心疾患または外傷性脳脊髄神経障害の治療のための細胞製剤。
[請求項10]
請求項1~8の何れか1項に記載の方法により生成された培養物を含む、請求項9に記載の細胞製剤。
[請求項11]
請求項1~8の何れか1項に記載の方法により生成された培養物を含む、血管新生の促進または軸索伸展の促進のための細胞製剤。
[請求項12]
ミクログリアおよび/または単球を含む細胞群を、低酸素濃度および/または低糖濃度の条件下で培養することにより生成された、治療上有効量のミクログリアおよび/または単球を含む培養物を、対象に投与することを含む、脳血管障害、虚血性心疾患または外傷性脳脊髄神経障害の治療方法。
[請求項13]
脳血管障害、虚血性心疾患または外傷性脳脊髄神経障害の治療における使用のための、低酸素濃度および/または低糖濃度の条件下で培養されたミクログリアおよび/または単球。
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • NIIGATA UNIVERSITY
  • Inventor
  • SHIMOHATA, Takayoshi
  • KANAZAWA, Masato
IPC(International Patent Classification)
Specified countries National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
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