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METHOD AND TEST PIECE FOR TESTING FOR KAWASAKI DISEASE

Foreign code F180009396
File No. (S2016-1017-N0)
Posted date Apr 20, 2018
Country WIPO
International application number 2017JP028236
International publication number WO 2018030270
Date of international filing Aug 3, 2017
Date of international publication Feb 15, 2018
Priority data
  • P2016-156241 (Aug 9, 2016) JP
Title METHOD AND TEST PIECE FOR TESTING FOR KAWASAKI DISEASE
Abstract To provide a method and test piece for quickly and easily testing for Kawasaki disease. A method for testing for Kawasaki disease characterized by measuring the levels of lipopolysaccharide-binding protien (LBP) and leucine-rich α2-glycoprotien (LRG1) in a sample originating from a subject to be tested. A test piece for detecting Kawasaki disease through an immunochromatography method, the test piece containing carriers on which anti-LBP antibodies are fixed, and carriers on which anti-LRG1 antibodies are fixed.
Outline of related art and contending technology BACKGROUND ART
4 Years old or younger infants kawasaki disease is mainly found in acute febrile rash and disease, systemic vasculitis in the main body of the condition. Kawasaki disease diagnosis of symptoms of the plurality of primary (1. 5 or more subsequent heating, 2. on both sides of the bulbar conjunctiva of the congestion, 3. lip redness, concentrate the tongue, 4. of the irregular shaped rash, 5. acute phase of the hand fingers of the rigid, hand palms and soles erythema, antipyretic of the film-like desquamation, purulent lymphadenopathy non-of the neck 6.) performed by the appearance of (kawasaki disease diagnosis guidance). In the blood, white blood cell count, C-reactive protein, without departing from the increase of the enzyme in the liver cells, enhancement of erythrocyte sedimentation, such as white blood cells (neutrophils ratio) the fractions is checked, two-dimensional echocardiography or cardiovascular imaging method such as a check of coronary lesions has also been performed.
Kawasaki disease is a disease in a natural a sophisticated, non-treatment of after the elapse of the 25-30% coronary lesions in the patient's cardiac complications typically occur. Therefore, in developing a treatment was initiated early kawasaki disease, inflammation and sedation is important, and have even 1 heat period can be shortened, it is possible to prevent the occurrence of complications of the heart is needed. However, the mechanism of the development and pathogenesis kawasaki disease is not known yet and, instead of the specific diagnostic tests, and for the major symptoms of the individual difference, there are a multitude of example does not meet the diagnostic criteria. Therefore, rapid definitive diagnosis is difficult kawasaki disease.
In addition, the diagnosis of patents kawasaki disease, blood of VEGF (Vascular Endothelial Growth Factor: vascular endothelial growth factor) a method of measuring concentration (Patent Document 1: JP-11-6832), 1 or IgM antigen a method of measuring a plurality of super (Patent Document 2: JP-3-139294), other, the investigation of genetic polymorphism (Patent Document 3: JP-2009-72193) and the like, a clinical site is actually not yet been used.
Scope of claims (In Japanese)[請求項1]
被験者由来の検体中のリポ多糖結合タンパク質(LBP)及びロイシンリッチα2-グリコプロテイン(LRG1)のレベルを測定することを特徴とする川崎病の検査方法。
[請求項2]
LBPのレベル及びLRG1のレベルが、それぞれ、所定の値より高い場合に、川崎病に罹患している可能性が高いと判定し、また、所定の値より低い場合に、川崎病に罹患している可能性が低いと判定する請求項1記載の方法。
[請求項3]
被験者由来の検体が、血清、全血又は血漿である請求項1又は2に記載の方法。
[請求項4]
前記LBPのレベル及びLRG1のレベルの測定が、イムノクロマトグラフ法であることを特徴とする請求項1~3のいずれかに記載の川崎病の検査方法。
[請求項5]
抗LBP抗体が固定化されている担体及び抗LRG1抗体が固定化されている担体を含む、イムノクロマト法により川崎病を検出するための試験片。
[請求項6]
抗LBP抗体が固定化されている担体と抗LRG1抗体が固定化されている担体とが、同じ試験片に含まれる請求項5記載の試験片。
[請求項7]
抗LBP抗体が固定化されている担体と抗LRG1抗体が固定化されている担体とが、別々の試験片に含まれる請求項5記載の試験片。
  • Applicant
  • ※All designated countries except for US in the data before July 2012
  • YOKOHAMA CITY UNIVERSITY
  • Inventor
  • HORIUCHI, Yayoi
  • MORI, Masaaki
  • HIRANO, Hisashi
  • SAITO, Yoko
IPC(International Patent Classification)
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