TOP > 外国特許検索 > Substance-containing vesicle, and production method therefor

Substance-containing vesicle, and production method therefor

外国特許コード F180009415
整理番号 AF12-14US3
掲載日 2018年4月20日
出願国 アメリカ合衆国
出願番号 201715684086
公報番号 20180008550
出願日 平成29年8月23日(2017.8.23)
公報発行日 平成30年1月11日(2018.1.11)
国際出願番号 JP2014055186
国際公開番号 WO2014133172
国際出願日 平成26年2月28日(2014.2.28)
国際公開日 平成26年9月4日(2014.9.4)
優先権データ
  • 特願2013-041186 (2013.3.1) JP
  • 特願2013-176068 (2013.8.27) JP
  • 2014WO-JP55186 (2014.2.28) WO
  • 2015US-14839854 (2015.8.28) US
発明の名称 (英語) Substance-containing vesicle, and production method therefor
発明の概要(英語) (US20180008550)
Provided is a monodisperse agglomerate of a substance-containing vesicle filled with a substance at a concentration higher than conventionally possible.
A mixed solution, in which a target substance is included in an aqueous medium, is mixed with a monodisperse agglomerate of a crosslinked vesicle comprising a prescribed polymer which includes a first polymer, i.e. a block copolymer having uncharged hydrophilic segments and first charged segments, and a second polymer having second charged segments carrying a charge opposite to that of the first charged segments, and in which the first polymer and/or the second polymer are/is crosslinked.
As a result, the crosslinked vesicle is made to contain the target substance.
特許請求の範囲(英語) [claim1]
1. A method of producing a target substance-encapsulating vesicle, comprising the steps of: (a) obtaining an enzyme-encapsulating vesicle comprising a membrane containing a first polymer, which is a block copolymer having an uncharged hydrophilic segment and a first charged segment, and a second polymer, which has a second charged segment having a charge opposite to the charge of the first charged segment, and an enzyme encapsulated in the vesicle; and
(b) mixing the enzyme-encapsulating vesicle with a precursor of a target substance in an aqueous medium under conditions which provide a lower water solubility for the target substance than for the precursor such that when the enzyme converts the precursor into the target substance, the target substance precipitates and is encapsulated in the enzyme-encapsulating vesicle, thereby forming the target substance-encapsulating vesicle.
[claim2]
2. The method according to claim 1, wherein step (b) is carried out by mixing the enzyme-encapsulating vesicle with an aqueous solution of the precursor.
[claim3]
3. The method according to claim 2, further comprising crosslinking the first and/or the second polymer(s) of the enzyme-encapsulating vesicle before step (b).
[claim4]
4. A substance-encapsulating vesicle produced by the method according to claim 1.
[claim5]
5. A substance-encapsulating vesicle comprising: a vesicle comprising a membrane containing a first polymer, which is a block copolymer having an uncharged hydrophilic segment and a first charged segment, and a second polymer, which has a second charged segment having a charge opposite to the charge of the first charged segment;
a substance encapsulated in the vesicle at a concentration exceeding the solubility of the substance to the inner aqueous phase.
[claim6]
6. The substance-encapsulating vesicle according to claim 5, wherein the first and/or the second polymer(s) is(are) crosslinked.
[claim7]
7. A drug delivery system comprising the vesicle according to claim 4.
[claim8]
8. A drug delivery system comprising the vesicle according to claim 5.
[claim9]
9. A method of delivering a drug to a subject, comprising the steps of: (a) obtaining an enzyme-encapsulating vesicle comprising a membrane containing a first polymer, which is a block copolymer having an uncharged hydrophilic segment and a first charged segment, and a second polymer, which has a second charged segment having a charge opposite to the charge of the first charged segment, and an enzyme encapsulated in the vesicle;
(b) delivering the enzyme-encapsulating vesicle to a desired site in a subject; and
(c) introducing a prodrug into the enzyme-encapsulating vesicle at the desired site in the subject such that when the enzyme converts the prodrug into a drug, the drug is encapsulated in the enzyme-encapsulating vesicle, thereby forming the drug-encapsulating vesicle.
[claim10]
10. The method according to claim 9, wherein the prodrug has a lower water-solubility than the drug, the prodrug is introduced into the enzyme-encapsulating vesicle in step (c) under conditions providing a lower water solubility for the drug than for the prodrug such that when the enzyme converts the prodrug into the drug, the drug precipitates and is encapsulated in the enzyme-encapsulating vesicle, thereby forming the drug-encapsulating vesicle.
  • 発明者/出願人(英語)
  • KATAOKA KAZUNORI
  • KISHIMURA AKIHIRO
  • ANRAKU YASUTAKA
  • GOTO AKINORI
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
参考情報 (研究プロジェクト等) CREST Establishment of Innovative Manufacturing Technology Based on Nanoscience AREA
ライセンスをご希望の方、特許の内容に興味を持たれた方は、問合せボタンを押してください。

PAGE TOP

close
close
close
close
close
close