Method for producing coenzyme and transformant set for coenzyme production
| 外国特許コード | F180009416 |
|---|---|
| 整理番号 | AF30-03WO |
| 掲載日 | 2018年4月24日 |
| 出願国 | アメリカ合衆国 |
| 出願番号 | 201615552440 |
| 公報番号 | 20180044683 |
| 出願日 | 平成28年2月19日(2016.2.19) |
| 公報発行日 | 平成30年2月15日(2018.2.15) |
| 国際出願番号 | JP2016054872 |
| 国際公開番号 | WO2016136620 |
| 国際出願日 | 平成28年2月19日(2016.2.19) |
| 国際公開日 | 平成28年9月1日(2016.9.1) |
| 優先権データ |
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| 発明の名称 （英語） | Method for producing coenzyme and transformant set for coenzyme production |
| 発明の概要（英語） |
(US20180044683) The present invention provides a method for synthesizing NAD+ or NADH in an enzyme reaction system requiring NAD+ or NADH so as to supplement NAD+ lost due to thermal decomposition in the enzyme reaction system, and a transformant set for coenzyme production used in the method. That is, the present invention relates to a method for producing a coenzyme including performing synthesis of NAD+ or NADH in an enzyme reaction system requiring NAD+ or NADH by adding one or more thermostable enzymes required for a reaction for synthesizing NAD+ from nicotinamide, to the enzyme reaction system, and a transformant set for coenzyme production including one or more transformants into which one or more genes encoding thermostable enzymes required for a reaction for synthesizing NAD+ or NADH from nicotinamide are introduced by using a non-thermostable microorganism as a host. |
| 特許請求の範囲（英語） |
[claim1] 1. A method for producing a coenzyme, the method comprising: performing synthesis of NAD+ in an enzyme reaction system requiring NAD+ by adding one or more thermostable enzymes required for a reaction for synthesizing NAD+ from ADP-ribose and nicotinamide, to the enzyme reaction system. [claim2] 2. The method for producing a coenzyme according to claim 1, wherein the thermostable enzymes required for a reaction for synthesizing NAD+ are nicotinamidase, nicotinate phosphoribosyltransferase, nicotinate-nucleotide adenylyltransferase, NAD+ synthase, ADP-ribose pyrophosphatase, and ribose-phosphate pyrophosphokinase. [claim3] 3. A method for producing a coenzyme, the method comprising: performing synthesis of NADH in an enzyme reaction system requiring NADH by adding one or more thermostable enzymes required for a reaction for synthesizing NADH from ADP-ribose and nicotinamide, to the enzyme reaction system. [claim4] 4. The method for producing a coenzyme according to claim 3, wherein the thermostable enzymes required for a reaction for synthesizing NADH are nicotinamidase, nicotinate phosphoribosyltransferase, nicotinate-nucleotide adenylyltransferase, NAD+ synthase, ADP-ribose pyrophosphatase, ribose-phosphate pyrophosphokinase, and oxidoreductase that catalyzes a reaction for synthesizing NADH from NAD+. [claim5] 5. The method for producing a coenzyme according to claim 4, wherein the oxidoreductase is dehydrogenase with sugar, alcohol, or organic acid as a substrate. [claim6] 6. The method for producing a coenzyme according to claim 1, further comprising: performing synthesis of ATP in the enzyme reaction system by adding one or more thermostable enzymes required for a reaction for synthesizing ATP from AMP or ADP, to the enzyme reaction system. [claim7] 7. The method for producing a coenzyme according to claim 6, wherein the thermostable enzymes required for a reaction for synthesizing ATP are adenylate kinase and polyphosphate kinase. [claim8] 8. The method for producing a coenzyme according to claim 1, wherein the thermostable enzyme is synthesized by an expression system of a non-thermostable microorganism. [claim9] 9. The method for producing a coenzyme according to claim 1, wherein the thermostable enzyme is synthesized by a transformant into which a gene encoding the thermostable enzyme is introduced by using a non-thermostable microorganism as a host. [claim10] 10. The method for producing a coenzyme according to claim 9, wherein a heat treated product of microbial cells obtained by culturing the transformant is added to the enzyme reaction system. [claim11] 11. The method for producing a coenzyme according to claim 8, wherein the non-thermostable microorganism is E. coli. [claim12] 12. A transformant set for coenzyme production, comprising: one or more transformants into which one or more genes encoding thermostable enzymes required for a reaction for synthesizing NAD+ or NADH from ADP-ribose and nicotinamide are introduced by using a non-thermostable microorganism as a host. [claim13] 13. The transformant set for coenzyme production according to claim 12, wherein the thermostable enzymes are nicotinamidase, nicotinate phosphoribosyltransferase, nicotinate-nucleotide adenylyltransferase, NAD+ synthase, ADP-ribose pyrophosphatase, and ribose-phosphate pyrophosphokinase. [claim14] 14. The transformant set for coenzyme production according to claim 12, wherein the thermostable enzymes are nicotinamidase, nicotinate phosphoribosyltransferase, nicotinate-nucleotide adenylyhransferase, NAD+ synthase, ADP-ribose pyrophosphatase, ribose-phosphate pyrophosphokinase, and oxidoreductase that catalyzes a reaction for synthesizing NADH from NAD+. [claim15] 15. The transformant set for coenzyme production according to claim 12, further comprising: one or more transformants into which one or more genes encoding thermostable enzymes required for a reaction for synthesizing ATP from AMP or ADP are introduced by using a non-thermostable microorganism as a host. [claim16] 16. The transformant set for coenzyme production according to claim 12, wherein one type of genes encoding the thermostable enzymes is introduced into one transformant. [claim17] 17. The transformant set for coenzyme production according to claim 12, wherein the non-thermostable microorganism is E. coli. [claim18] 18. The transformant set for coenzyme production according to claim 12, wherein the transformant is heat treated. [claim19] 19. The method for producing a coenzyme according to claim 3, further comprising: performing synthesis of ATP in the enzyme reaction system by adding one or more thermostable enzymes required for a reaction for synthesizing ATP from AMP or ADP, to the enzyme reaction system. [claim20] 20. The method for producing a coenzyme according to claim 19, wherein the thermostable enzymes required for a reaction for synthesizing ATP are adenylate kinase and polyphosphate kinase. |
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| 国際特許分類(IPC) |
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| 参考情報 （研究プロジェクト等） | CREST Creation of Basic Technology for Improved Bioenergy Production through Functional Analysis and Regulation of Algae and Other Aquatic Microorganisms AREA |
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