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Method for producing coenzyme and transformant set for coenzyme production

外国特許コード F180009416
整理番号 AF30-03WO
掲載日 2018年4月24日
出願国 アメリカ合衆国
出願番号 201615552440
公報番号 20180044683
出願日 平成28年2月19日(2016.2.19)
公報発行日 平成30年2月15日(2018.2.15)
国際出願番号 JP2016054872
国際公開番号 WO2016136620
国際出願日 平成28年2月19日(2016.2.19)
国際公開日 平成28年9月1日(2016.9.1)
優先権データ
  • 特願2015-033843 (2015.2.24) JP
  • 2016WO-JP54872 (2016.2.19) WO
発明の名称 (英語) Method for producing coenzyme and transformant set for coenzyme production
発明の概要(英語) (US20180044683)
The present invention provides a method for synthesizing NAD+ or NADH in an enzyme reaction system requiring NAD+ or NADH so as to supplement NAD+ lost due to thermal decomposition in the enzyme reaction system, and a transformant set for coenzyme production used in the method.
That is, the present invention relates to a method for producing a coenzyme including performing synthesis of NAD+ or NADH in an enzyme reaction system requiring NAD+ or NADH by adding one or more thermostable enzymes required for a reaction for synthesizing NAD+ from nicotinamide, to the enzyme reaction system, and a transformant set for coenzyme production including one or more transformants into which one or more genes encoding thermostable enzymes required for a reaction for synthesizing NAD+ or NADH from nicotinamide are introduced by using a non-thermostable microorganism as a host.
特許請求の範囲(英語) [claim1]
1. A method for producing a coenzyme, the method comprising: performing synthesis of NAD+ in an enzyme reaction system requiring NAD+ by adding one or more thermostable enzymes required for a reaction for synthesizing NAD+ from ADP-ribose and nicotinamide, to the enzyme reaction system.
[claim2]
2. The method for producing a coenzyme according to claim 1, wherein the thermostable enzymes required for a reaction for synthesizing NAD+ are nicotinamidase, nicotinate phosphoribosyltransferase, nicotinate-nucleotide adenylyltransferase, NAD+ synthase, ADP-ribose pyrophosphatase, and ribose-phosphate pyrophosphokinase.
[claim3]
3. A method for producing a coenzyme, the method comprising: performing synthesis of NADH in an enzyme reaction system requiring NADH by adding one or more thermostable enzymes required for a reaction for synthesizing NADH from ADP-ribose and nicotinamide, to the enzyme reaction system.
[claim4]
4. The method for producing a coenzyme according to claim 3, wherein the thermostable enzymes required for a reaction for synthesizing NADH are nicotinamidase, nicotinate phosphoribosyltransferase, nicotinate-nucleotide adenylyltransferase, NAD+ synthase, ADP-ribose pyrophosphatase, ribose-phosphate pyrophosphokinase, and oxidoreductase that catalyzes a reaction for synthesizing NADH from NAD+.
[claim5]
5. The method for producing a coenzyme according to claim 4, wherein the oxidoreductase is dehydrogenase with sugar, alcohol, or organic acid as a substrate.
[claim6]
6. The method for producing a coenzyme according to claim 1, further comprising: performing synthesis of ATP in the enzyme reaction system by adding one or more thermostable enzymes required for a reaction for synthesizing ATP from AMP or ADP, to the enzyme reaction system.
[claim7]
7. The method for producing a coenzyme according to claim 6, wherein the thermostable enzymes required for a reaction for synthesizing ATP are adenylate kinase and polyphosphate kinase.
[claim8]
8. The method for producing a coenzyme according to claim 1, wherein the thermostable enzyme is synthesized by an expression system of a non-thermostable microorganism.
[claim9]
9. The method for producing a coenzyme according to claim 1, wherein the thermostable enzyme is synthesized by a transformant into which a gene encoding the thermostable enzyme is introduced by using a non-thermostable microorganism as a host.
[claim10]
10. The method for producing a coenzyme according to claim 9, wherein a heat treated product of microbial cells obtained by culturing the transformant is added to the enzyme reaction system.
[claim11]
11. The method for producing a coenzyme according to claim 8, wherein the non-thermostable microorganism is E. coli.
[claim12]
12. A transformant set for coenzyme production, comprising: one or more transformants into which one or more genes encoding thermostable enzymes required for a reaction for synthesizing NAD+ or NADH from ADP-ribose and nicotinamide are introduced by using a non-thermostable microorganism as a host.
[claim13]
13. The transformant set for coenzyme production according to claim 12, wherein the thermostable enzymes are nicotinamidase, nicotinate phosphoribosyltransferase, nicotinate-nucleotide adenylyltransferase, NAD+ synthase, ADP-ribose pyrophosphatase, and ribose-phosphate pyrophosphokinase.
[claim14]
14. The transformant set for coenzyme production according to claim 12, wherein the thermostable enzymes are nicotinamidase, nicotinate phosphoribosyltransferase, nicotinate-nucleotide adenylyhransferase, NAD+ synthase, ADP-ribose pyrophosphatase, ribose-phosphate pyrophosphokinase, and oxidoreductase that catalyzes a reaction for synthesizing NADH from NAD+.
[claim15]
15. The transformant set for coenzyme production according to claim 12, further comprising: one or more transformants into which one or more genes encoding thermostable enzymes required for a reaction for synthesizing ATP from AMP or ADP are introduced by using a non-thermostable microorganism as a host.
[claim16]
16. The transformant set for coenzyme production according to claim 12, wherein one type of genes encoding the thermostable enzymes is introduced into one transformant.
[claim17]
17. The transformant set for coenzyme production according to claim 12, wherein the non-thermostable microorganism is E. coli.
[claim18]
18. The transformant set for coenzyme production according to claim 12, wherein the transformant is heat treated.
[claim19]
19. The method for producing a coenzyme according to claim 3, further comprising: performing synthesis of ATP in the enzyme reaction system by adding one or more thermostable enzymes required for a reaction for synthesizing ATP from AMP or ADP, to the enzyme reaction system.
[claim20]
20. The method for producing a coenzyme according to claim 19, wherein the thermostable enzymes required for a reaction for synthesizing ATP are adenylate kinase and polyphosphate kinase.
  • 発明者/出願人(英語)
  • HONDA KOHSUKE
  • ATOMI HARUYUKI
  • JAPAN SCIENCE AND TECHNOLOGY AGENCY
国際特許分類(IPC)
参考情報 (研究プロジェクト等) CREST Creation of Basic Technology for Improved Bioenergy Production through Functional Analysis and Regulation of Algae and Other Aquatic Microorganisms AREA
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