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Method and apparatus for quantitative evaluation of wall zeta-potential, and method and apparatus for quantitative visualization of surface modification pattern UPDATE 新技術説明会

外国特許コード F180009424
整理番号 P1U1-1535
掲載日 2018年6月25日
出願国 アメリカ合衆国
出願番号 200913120041
公報番号 20110174988
公報番号 8592781
出願日 平成21年9月25日(2009.9.25)
公報発行日 平成23年7月21日(2011.7.21)
公報発行日 平成25年11月26日(2013.11.26)
国際出願番号 JP2009066652
国際公開番号 WO2010041560
国際出願日 平成21年9月25日(2009.9.25)
国際公開日 平成22年4月15日(2010.4.15)
優先権データ
  • 特願2008-263639 (2008.10.10) JP
  • 2009WO-JP66652 (2009.9.25) WO
発明の名称 (英語) Method and apparatus for quantitative evaluation of wall zeta-potential, and method and apparatus for quantitative visualization of surface modification pattern UPDATE 新技術説明会
発明の概要(英語) (US8592781)
A first and a second fluorescent dye are mixed into a solution, the first dye being positively ionized in the solution and the second dye being negatively ionized in the solution and having different fluorescence wavelengths from the first dye.
The solution is flown onto a measured surface, and the surface is excited with an evanescent wave to produce a fluorescence intensity distribution of two colors.
A fluorescence intensity of the surface is measured using a two-dimensional imaging element, the element providing a fluorescence intensity of each color separated from the other colors, thereby calculating a ratio of the fluorescence intensities of the colors.
Using an equation expressing a relationship between the ratio of fluorescence intensities and a wall zeta potential, the ratio is converted to a two-dimensional distribution of wall zeta potentials.
This achieves visualizing in real time and quantitatively evaluating the two-dimensional distribution of wall zeta potentials, and surface modifications.
特許請求の範囲(英語) [claim1]
1. A method for quantitative evaluation of a wall zeta potential, pH, or temperature distribution, the method comprising: a step of mixing a first fluorescent dye and a second fluorescent dye into a solution, the first dye being positively ionized in the solution, and the second dye being negatively ionized in the solution and having a different fluorescence wavelength from the first dye;
a step of flowing the solution onto a measured surface;
a step of exciting the measured surface with an evanescent wave to thereby produce a fluorescence intensity distribution of two colors according to a concentration distribution of each fluorescent dye;
a step of measuring a fluorescence intensity of the measured surface using a two-dimensional imaging element, the element being capable of providing a fluorescence intensity of each color separated from fluorescence intensities of the two colors;
a step of calculating a ratio of the fluorescence intensities of the two colors; and
a step of using a previously created equation expressing a relationship between the ratio of fluorescence intensities and a wall zeta potential, pH, or temperature to convert the distribution of the ratio of fluorescence intensities into a two-dimensional distribution of wall zeta potentials, pHs, or temperatures.
[claim2]
2. The method for quantitative evaluation of a wall zeta potential, pH, or temperature distribution according to claim 1, wherein the relationship between the fluorescence intensity ratio and the wall zeta potential, pH, or temperature is calibrated.
[claim3]
3. The method for quantitative evaluation of a wall zeta potential, pH, or temperature distribution according to claim 1, wherein the first dye is dichlorotris(1,10-phenanthroline) ruthenium(II) hydrate, which emits red light, and the second dye emits green light.
[claim4]
4. The method for quantitative evaluation of a wall zeta potential, pH, or temperature distribution according to claim 1, wherein, the two-dimensional imaging element is a single 3CCD camera.
[claim5]
5. An apparatus for quantitative evaluation of a distribution of wall zeta potentials, pHs, or temperatures, the apparatus comprising: means for flowing a solution mixed with a first fluorescent dye and a second fluorescent dye, the first dye being positively ionized in the solution, and the second dye being negatively ionized in the solution and having a different fluorescence wavelength from the first dye, onto a measured surface;
means for exciting the measured surface with an evanescent wave to thereby produce a fluorescence intensity distribution of two colors according to a concentration distribution of each fluorescent dye;
a two-dimensional imaging element being capable of providing a fluorescence intensity of each color separated from fluorescence intensities of two colors;
means for calculating a ratio of the fluorescence intensities of the plurality of colors measured using the two-dimensional imaging element; and
means for using a previously created equation expressing a relationship between the ratio of fluorescence intensities and a wall zeta potential, pH, or temperature to convert the distribution of the ratio of fluorescence intensities into a two-dimensional distribution of wall zeta potentials, pHs, or temperatures.
[claim6]
6. A method for quantitative visualization of a surface modification, the method comprising: a step of mixing a first fluorescent dye and a second fluorescent dye into a solution, the first dye being positively ionized in the solution, and the second dye being negatively ionized in the solution and having a different fluorescence wavelength from the first dye;
a step of flowing the solution onto a measured surface, the measured surface having a localized distribution of zeta potentials, pHs, or temperatures due to a surface modification;
a step of exciting the measured surface with an evanescent wave to thereby produce a fluorescence intensity distribution of two colors according to a concentration distribution of each fluorescent dye;
a step of measuring a fluorescence intensity of the measured surface using a two-dimensional imaging element, the element being capable of providing a fluorescence intensity of each color separated from fluorescence intensities of the two colors;
a step of calculating a ratio of the fluorescence intensities of the two colors; and
a step of using a previously created equation expressing a relationship between the ratio of fluorescence intensities and a wall zeta potential, pH, or temperature to convert the distribution of the ratio of fluorescence intensities into a two-dimensional distribution of wall zeta potentials, pHs, or temperatures, thereby visualizing a surface modification pattern.
[claim7]
7. The method for quantitative visualization of a surface modification according to claim 6, wherein the surface modification is made by octadecyltrichlorosilane.
[claim8]
8. An apparatus for quantitative visualization of a surface modification, the apparatus comprising: means for flowing a solution mixed with a first fluorescent dye and a second fluorescent dye, the first dye being positively ionized in the solution, and the second dye being negatively ionized in the solution and having a different fluorescence wavelength from the first dye, onto a measured surface, the measured surface having a localized distribution of zeta potentials, pHs, or temperatures due to a surface modification;
means for exciting the measured surface with an evanescent wave to thereby produce a fluorescence intensity distribution of two colors according to a concentration distribution of each fluorescent dye;
a two-dimensional imaging element being capable of providing a fluorescence intensity of each color separated from fluorescence intensities of the two colors,
means for calculating a ratio of the fluorescence intensities of multiple colors measured using the two-dimensional imaging element; and
means for using a previously created equation expressing a relationship between the ratio of fluorescence intensities and a wall zeta potential, pH, or temperature to convert the distribution of the ratio of fluorescence intensities into a two-dimensional distribution of wall zeta potentials, pHs, or temperatures, thereby visualizing a surface modification pattern.
  • 発明者/出願人(英語)
  • SATO YOHEI
  • KAZOE YUTAKA
  • MIYAKAWA SHU
  • KEIO UNIVERSITY
国際特許分類(IPC)
参考情報 (研究プロジェクト等) Hishida-Sato Laboratory
Department of System Design Engineering,Keio University
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