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Method and apparatus for quantitative evaluation of wall zeta-potential, and method and apparatus for quantitative visualization of surface modification pattern meetings

Foreign code F180009424
File No. P1U1-1535
Posted date Jun 25, 2018
Country United States of America
Application number 200913120041
Gazette No. 20110174988
Gazette No. 8592781
Date of filing Sep 25, 2009
Gazette Date Jul 21, 2011
Gazette Date Nov 26, 2013
International application number JP2009066652
International publication number WO2010041560
Date of international filing Sep 25, 2009
Date of international publication Apr 15, 2010
Priority data
  • P2008-263639 (Oct 10, 2008) JP
  • 2009WO-JP66652 (Sep 25, 2009) WO
Title Method and apparatus for quantitative evaluation of wall zeta-potential, and method and apparatus for quantitative visualization of surface modification pattern meetings
Abstract (US8592781)
A first and a second fluorescent dye are mixed into a solution, the first dye being positively ionized in the solution and the second dye being negatively ionized in the solution and having different fluorescence wavelengths from the first dye.
The solution is flown onto a measured surface, and the surface is excited with an evanescent wave to produce a fluorescence intensity distribution of two colors.
A fluorescence intensity of the surface is measured using a two-dimensional imaging element, the element providing a fluorescence intensity of each color separated from the other colors, thereby calculating a ratio of the fluorescence intensities of the colors.
Using an equation expressing a relationship between the ratio of fluorescence intensities and a wall zeta potential, the ratio is converted to a two-dimensional distribution of wall zeta potentials.
This achieves visualizing in real time and quantitatively evaluating the two-dimensional distribution of wall zeta potentials, and surface modifications.
Scope of claims [claim1]
1. A method for quantitative evaluation of a wall zeta potential, pH, or temperature distribution, the method comprising: a step of mixing a first fluorescent dye and a second fluorescent dye into a solution, the first dye being positively ionized in the solution, and the second dye being negatively ionized in the solution and having a different fluorescence wavelength from the first dye;
a step of flowing the solution onto a measured surface;
a step of exciting the measured surface with an evanescent wave to thereby produce a fluorescence intensity distribution of two colors according to a concentration distribution of each fluorescent dye;
a step of measuring a fluorescence intensity of the measured surface using a two-dimensional imaging element, the element being capable of providing a fluorescence intensity of each color separated from fluorescence intensities of the two colors;
a step of calculating a ratio of the fluorescence intensities of the two colors; and
a step of using a previously created equation expressing a relationship between the ratio of fluorescence intensities and a wall zeta potential, pH, or temperature to convert the distribution of the ratio of fluorescence intensities into a two-dimensional distribution of wall zeta potentials, pHs, or temperatures.
[claim2]
2. The method for quantitative evaluation of a wall zeta potential, pH, or temperature distribution according to claim 1, wherein the relationship between the fluorescence intensity ratio and the wall zeta potential, pH, or temperature is calibrated.
[claim3]
3. The method for quantitative evaluation of a wall zeta potential, pH, or temperature distribution according to claim 1, wherein the first dye is dichlorotris(1,10-phenanthroline) ruthenium(II) hydrate, which emits red light, and the second dye emits green light.
[claim4]
4. The method for quantitative evaluation of a wall zeta potential, pH, or temperature distribution according to claim 1, wherein, the two-dimensional imaging element is a single 3CCD camera.
[claim5]
5. An apparatus for quantitative evaluation of a distribution of wall zeta potentials, pHs, or temperatures, the apparatus comprising: means for flowing a solution mixed with a first fluorescent dye and a second fluorescent dye, the first dye being positively ionized in the solution, and the second dye being negatively ionized in the solution and having a different fluorescence wavelength from the first dye, onto a measured surface;
means for exciting the measured surface with an evanescent wave to thereby produce a fluorescence intensity distribution of two colors according to a concentration distribution of each fluorescent dye;
a two-dimensional imaging element being capable of providing a fluorescence intensity of each color separated from fluorescence intensities of two colors;
means for calculating a ratio of the fluorescence intensities of the plurality of colors measured using the two-dimensional imaging element; and
means for using a previously created equation expressing a relationship between the ratio of fluorescence intensities and a wall zeta potential, pH, or temperature to convert the distribution of the ratio of fluorescence intensities into a two-dimensional distribution of wall zeta potentials, pHs, or temperatures.
[claim6]
6. A method for quantitative visualization of a surface modification, the method comprising: a step of mixing a first fluorescent dye and a second fluorescent dye into a solution, the first dye being positively ionized in the solution, and the second dye being negatively ionized in the solution and having a different fluorescence wavelength from the first dye;
a step of flowing the solution onto a measured surface, the measured surface having a localized distribution of zeta potentials, pHs, or temperatures due to a surface modification;
a step of exciting the measured surface with an evanescent wave to thereby produce a fluorescence intensity distribution of two colors according to a concentration distribution of each fluorescent dye;
a step of measuring a fluorescence intensity of the measured surface using a two-dimensional imaging element, the element being capable of providing a fluorescence intensity of each color separated from fluorescence intensities of the two colors;
a step of calculating a ratio of the fluorescence intensities of the two colors; and
a step of using a previously created equation expressing a relationship between the ratio of fluorescence intensities and a wall zeta potential, pH, or temperature to convert the distribution of the ratio of fluorescence intensities into a two-dimensional distribution of wall zeta potentials, pHs, or temperatures, thereby visualizing a surface modification pattern.
[claim7]
7. The method for quantitative visualization of a surface modification according to claim 6, wherein the surface modification is made by octadecyltrichlorosilane.
[claim8]
8. An apparatus for quantitative visualization of a surface modification, the apparatus comprising: means for flowing a solution mixed with a first fluorescent dye and a second fluorescent dye, the first dye being positively ionized in the solution, and the second dye being negatively ionized in the solution and having a different fluorescence wavelength from the first dye, onto a measured surface, the measured surface having a localized distribution of zeta potentials, pHs, or temperatures due to a surface modification;
means for exciting the measured surface with an evanescent wave to thereby produce a fluorescence intensity distribution of two colors according to a concentration distribution of each fluorescent dye;
a two-dimensional imaging element being capable of providing a fluorescence intensity of each color separated from fluorescence intensities of the two colors,
means for calculating a ratio of the fluorescence intensities of multiple colors measured using the two-dimensional imaging element; and
means for using a previously created equation expressing a relationship between the ratio of fluorescence intensities and a wall zeta potential, pH, or temperature to convert the distribution of the ratio of fluorescence intensities into a two-dimensional distribution of wall zeta potentials, pHs, or temperatures, thereby visualizing a surface modification pattern.
  • Inventor, and Inventor/Applicant
  • SATO YOHEI
  • KAZOE YUTAKA
  • MIYAKAWA SHU
  • KEIO UNIVERSITY
IPC(International Patent Classification)
Reference ( R and D project ) Hishida-Sato Laboratory
Department of System Design Engineering,Keio University
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