TOP > 外国特許検索 > METHOD FOR PRODUCING NERVE CELLS FROM HUMAN NEURAL STEM CELLS BY DIFFERENTIATION INDUCTION TREATMENT, REAGENT FOR INDUCING DIFFERENTIATION OF NERVE CELLS, AND NERVE CELL DIFFERENTIATION INDUCTION KIT

METHOD FOR PRODUCING NERVE CELLS FROM HUMAN NEURAL STEM CELLS BY DIFFERENTIATION INDUCTION TREATMENT, REAGENT FOR INDUCING DIFFERENTIATION OF NERVE CELLS, AND NERVE CELL DIFFERENTIATION INDUCTION KIT UPDATE コモンズ 新技術説明会

外国特許コード F180009476
整理番号 2018000986
掲載日 2018年9月18日
出願国 世界知的所有権機関(WIPO)
国際出願番号 2018JP024843
国際公開番号 WO 2019009205
国際出願日 平成30年6月29日(2018.6.29)
国際公開日 平成31年1月10日(2019.1.10)
優先権データ
  • 特願2017-131308 (2017.7.4) JP
発明の名称 (英語) METHOD FOR PRODUCING NERVE CELLS FROM HUMAN NEURAL STEM CELLS BY DIFFERENTIATION INDUCTION TREATMENT, REAGENT FOR INDUCING DIFFERENTIATION OF NERVE CELLS, AND NERVE CELL DIFFERENTIATION INDUCTION KIT UPDATE コモンズ 新技術説明会
発明の概要(英語) The present invention addresses the problem of providing a method for promoting the neural differentiation of neural stem cells. A method for producing nerve cells from human neural stem cells by a differentiation induction treatment, the method including a step of carrying out a differentiation induction treatment of human neural stem cells using a FAD-containing culture medium for nerve cell differentiation induction use.
従来技術、競合技術の概要(英語) BACKGROUND ART
Mammalian central nervous system neurons and glial cells are present in the produced from neural stem cells. The use of the property of neural stem cells, for the treatment of injured nervous system and realizes a regenerative medicine, drug screening in drug discovery, efficacy and toxicity evaluation of the contribution to the expected.
In recent years, such as a mouse or a rat neural stem cells of rodent, primate including human neural stem cells can be of different nature have been reported (Non-Patent Document 1 and 2). For example, rodent brain cortex of such as neural stem cells located in the band, only to excitatory neurons have differentiated, primate neural stem cells, excitatory neurons inhibitory nerve cell as well as the ability to differentiate into which have been reported.
For application to medical and drug discovery is to, human neural stem cells cultured in a stable, efficient technique for inducing differentiation to the nerve cells is required. Therefore, human neural stem cells efficient neural differentiation induction, in order to implement a reliable and simple method of development has been desired and, also, be molecular understanding of the mechanism has been desired.
For example, human neural stem cells to differentiate into neuronal cells from the as a method, in the non-patent document 3, for human neural stem cells from the culture solution, is applied in a stepwise manner between 18, B27 (1x) (20ng/ml) (registered trademark) and BDNF (registered trademark) was added to Neurobasal medium is replaced with a, human neural stem cell derived neuronal differentiation from the reported to be executed. In accordance with the method of induction, about 2 weeks, immature neurons (DCX) marker doublecortin, βIII-Tubulin be observed. However, this neuron and the induction efficiency is about 40%, sufficient induction efficiency can be obtained is far.
  • 出願人(英語)
  • ※2012年7月以前掲載分については米国以外のすべての指定国
  • NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
  • 発明者(英語)
  • HIRANO Kazumi
  • NAMIHIRA Masakazu
国際特許分類(IPC)
指定国 National States: AE AG AL AM AO AT AU AZ BA BB BG BH BN BR BW BY BZ CA CH CL CN CO CR CU CZ DE DJ DK DM DO DZ EC EE EG ES FI GB GD GE GH GM GT HN HR HU ID IL IN IR IS JO JP KE KG KH KN KP KR KW KZ LA LC LK LR LS LU LY MA MD ME MG MK MN MW MX MY MZ NA NG NI NO NZ OM PA PE PG PH PL PT QA RO RS RU RW SA SC SD SE SG SK SL SM ST SV SY TH TJ TM TN TR TT TZ UA UG US UZ VC VN ZA ZM ZW
ARIPO: BW GH GM KE LR LS MW MZ NA RW SD SL SZ TZ UG ZM ZW
EAPO: AM AZ BY KG KZ RU TJ TM
EPO: AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
OAPI: BF BJ CF CG CI CM GA GN GQ GW KM ML MR NE SN ST TD TG
参考情報 (研究プロジェクト等) Biomedical Research Institue,Molecular Neurophysiology Group

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